2012 Fiscal Year Final Research Report
Elucidation of pathogenesis and therapy development of sporadic ALS by disease model
| Project/Area Number |
22390175
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Yokohama City University (2012)
Nagoya University (2010-2011) |
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Principal Investigator |
TANAKA Fumiaki 横浜市立大学, 医学研究科, 教授 (30378012)
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| Co-Investigator(Kenkyū-buntansha) |
SOBUE Gen 名古屋大学, 医学系研究科, 教授 (20148315)
KATSUNO Masahisa 名古屋大学, 医学系研究科, 准教授 (50402566)
IIJIMA Masahiro 名古屋大学, 医学系研究科, 特任助教 (40437041)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ALS / 運動ニューロン / dynactin-1 / 線虫モデル / オートファジー |
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| Research Abstract |
In the present study, we generated a Caenorhabditis elegans model in which the expression of DNC-1, the homolog of dynactin 1, is specifically knocked down in motor neurons. This model exhibited severe motor defects together with axonal and neuronal degeneration. We also observed impaired movement and increased number of autophagosomes in the degenerated neurons. Furthermore, the combination of rapamycin, an activator of autophagy, and trichostatin which facilitates axonal transport dramatically ameliorated the motor phenotype and axonal degeneration of this model.
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[Journal Article] dnc-1/dynactin 1 knockdown disrupts transport of autophagosomes and induces motor neuron degeneration2013
Author(s)
Ikenaka K, Kawai K, Katsuno M, Huang Z, Jiang YM, Iguchi Y, Kobayashi K, Kimata T, Waza M, Tanaka F, Mori I, Sobue G
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Journal Title
PLoS One
Volume: 8
Pages: e54511
Peer Reviewed
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[Journal Article] Loss of TDP-43 causes age-dependent progressive motor neuron degeneration2012
Author(s)
Iguchi Y, Katsuno M, Niwa JI, Takagi S, Ishigaki S, Ikenaka K, Kawai K, Watanabe H, Yamanaka K, Takahashi R, Misawa H, Sasaki S, Tanaka F, Sobue G
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Journal Title
Brain
Volume: 136
Pages: 1371-1382
Peer Reviewed
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[Journal Article] Segmental copy-number gain within the region of isopentenyl diphosphate isomerase genes in sporadic amyotrophic lateral sclerosis2010
Author(s)
Kato T, Emi M, Sato H, Arawaka S, Wada M, Kawanami T, Katagiri T, Tsuburaya K, Toyoshima I, Tanaka F, Sobue G, Matsubara K
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Journal Title
Biophys Res Commun
Volume: 402
Pages: 438-442
Peer Reviewed
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