Study on the endoplasmic stress induced by oxsterol ester and its implication to diseases

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390187
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Jichi Medical University
• Principal Investigator: ISHIBASHI Shun 自治医科大学, 医学部, 教授 (90212919)
• Co-Investigator(Kenkyū-buntansha): OOSUGA Junniti 自治医科大学, 医学部, 准教授 (10334400) ; YAGYU Hiroaki 自治医科大学, 医学部, 准教授 (60348018)
• Project Period (FY): 2010 – 2012
• Keywords: 小胞体ストレス / 細胞死 / マクロファージ / コレステロール / オキシステロール

Research Abstract

NCEH1、an microsomal protein which hydrolizes cholesterol ester (CE), mediates the hydrolysis of esterified form of 25-hydroxycholesterol (25-HC), one of oxysterols. In human monocyte-derived macrophages, NCEH1 is responsible for the CE hydrolysis. Absence

Research Products

• [Journal Article] Macrophage lipoprotein lipase modulates the development of atherosclerosis but not adiposity (2013)
  • Author(s): Takahashi M, Yagyu H, Tazoe F, Nagashima S, Ohshiro T, Okada K, Osuga J, Goldberg IJ, Ishibashi S.
  • Journal Title: J Lipid Res Volume: 54(4) Pages: 1124-34
  • DOI: doi:10.1194/jlr.M035568.
• [Journal Article] Liver-specific deletion of 3-hydroxy-3-methylglutaryl coenzyme A reductase causes hepatic steatosis and death. (2012)
  • Author(s): Nagashima S, Yagyu H, Ohashi K, Tazoe F, Takahashi M, Ohshiro T, Bayasgalan T, Okada K, Sekiya M, Osuga J, Ishibashi S.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 32(8) Pages: 1824-31
  • DOI: doi:10.1161/ATVBAHA.111.240754.
• [Journal Article] Depot-specific expression of lipolytic genes in human adipose tissues-association among CES1 expression, triglyceride lipase activity and adiposity. (2011)
  • Author(s): Nagashima S, Yagyu H, Takahashi N, Kurashina T, Takahashi M, Tsuchita T, Tazoe F, Wang XL, Bayasgalan T, Sato N, Okada K, Nagasaka S, Gotoh T, Kojima M, Hyodo M, Horie H, Hosoya Y, Okada M, Yasuda Y, Fujiwara H, Ohwada M, Iwamoto S, Suzuki M, Nagai H, Ish
  • Journal Title: J Atheroscler Thromb. Volume: 18(3) Pages: 190-9
• [Journal Article] Abrogation of neutral cholesterol ester hydrolytic activity causes adrenal enlargement. (2011)
  • Author(s): Ohta K, Sekiya M, Uozaki H, Igarashi M, Takase S, Kumagai M, Takanashi M, Takeuchi Y, Izumida Y, Kubota M, Nishi M, Okazaki H, Iizuka Y, Yahagi N, Yagyu H, Fukayama M, Kadowaki T, Ohashi K, Ishibashi S, Osuga J
  • Journal Title: Biochem Biophys Res Commun. Volume: 404(1) Pages: 254-60
  • DOI: doi:10.1016/j.bbrc.2010.11.103.
• [Journal Article] an acyl-coenzyme A:cholesterol acyltransferase 2-selective inhibitor, attenuates hypercholesterolemia and atherosclerosis in murine models of hyperlipidemia. (2011)
  • Author(s): Ohshiro T, Matsuda D, Sakai K, Degirolamo C, Yagyu H, Rudel LL, Omura S, Ishibashi S, Tomoda H. Pyripyropene A
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 31(5) Pages: 1108-15
  • DOI: doi:10.1161/ATVBAHA.111.223552.
• [Journal Article] The critical role of neutral cholesterol ester hydrolase 1 in cholesterol removal from human macrophages. (2010)
  • Author(s): Igarashi M, Osuga J, Uozaki H, Sekiya M, Nagashima S, Takahashi M, Takase S, Takanashi M, Li Y, Ohta K, Kumagai M, Nishi M, Hosokawa M, Fledelius C, Jacobsen P, Yagyu H, Fukayama M, Nagai R, Kadowaki T, Ohashi K, Ishibashi S.
  • Journal Title: Circ Res. Volume: 107(11) Pages: 1387-95
  • DOI: doi:10.1161/CIRCRESAHA.110.226613.