The regenerative therapy for central diabetes insipidus

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390188
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Nagoya University
• Principal Investigator: OISO Yutaka 名古屋大学, 医学系研究科, 教授 (40203707)
• Co-Investigator(Kenkyū-buntansha): NAGASAKI Hiroshi 藤田保健衛生大学, 医学部, 准教授 (30420384) ; SUGA Hidetaka 名古屋大学, 医学部附属病院, 病院助教 (20569818)
• Project Period (FY): 2010 – 2012
• Keywords: 再生医学 / 中枢性尿崩症 / 生理活性

Research Abstract

It is known mouse embryonic stem cell can be developed into hypothalamic lineage (ES-hypo) invitro. In this study, we have improved the method to have vasopressin neurons more effectively. Central diabetes insipidus manifests polyuria and polydipsia which results from the deficit in the hypothalamic area that produce vasopressin. We have developed a regenerativetherapy using ES-hypo in the model animal, Brattleboro rat. The heterograft of ES-hypo in the brain survived more than six weeks, and recipients reduced urine volume by 40% compared to the negative control group.

Research Products

• [Journal Article] Lesion of area postrema attenuated hyperphagic responses to glucoprivation, but not transcriptional activation of the neuropeptide Y gene in rats. (2012)
  • Author(s): Ozawa Y, Arima H, Banno R, Ito Y, Goto M, Morishita Y, Sugimura Y, Ozaki N, Nagasaki H, Oiso Y
  • Journal Title: Neuroreport Volume: 23(11) Pages: 673-5
  • Peer Reviewed
• [Journal Article] BiP mRNA expression is upregulated by dehydration in vasopressin neurons in the hypothalamus in mice (2012)
  • Author(s): Hagiwara D, Arima H, Morishita Y, Goto M, Banno R, Sugimura Y, Oiso Y
  • Journal Title: Peptides Volume: 33(2) Pages: 346-50
  • Peer Reviewed
• [Journal Article] TNFαincreases hypothalamic PTP1B activity via the NFκB pathway in rat hypothalamic organotypic cultures (2012)
  • Author(s): Ito Y, Banno R, Hagimoto S, Ozawa Y, Arima H, Oiso Y.
  • Journal Title: Regul Pept Volume: 174(1-3) Pages: 58-64
  • Peer Reviewed
• [Journal Article] Self-formation of functional adenohypophysis in three-dimensional culture (2011)
  • Author(s): Suga H, Kadoshima T, Minaguchi M, Ohgushi M, Soen M, Nakano T, Takata N, Wataya T, Muguruma K, Miyoshi H, Yonemura S, Oiso Y, Sasai Y
  • Journal Title: Nature Volume: 480(7375) Pages: 57-62
  • Peer Reviewed
• [Journal Article] Poly(A)tail length of neurohypophysial hormones is shortened under endoplasmic reticulum stress. (2011)
  • Author(s): Morishita Y, Arima H, Hiroi M, Hayashi M, Hagiwara D, Asai N, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Takahashi M, Oiso Y
  • Journal Title: Endocrinology Volume: 152(12) Pages: 4846-55
  • Peer Reviewed
• [Journal Article] Repeated glucoprivation delayed hyperphagic responses while activating neuropeptide Y neurons in rats. (2011)
  • Author(s): Ozawa Y, Arima H, Watanabe M, Shimizu H, Ito Y, Banno R, Sugimura Y, Ozaki N, Nagasaki H, Oiso Y
  • Journal Title: Peptides Volume: 32(4) Pages: 763-9
  • Peer Reviewed
• [Journal Article] Time-dependent changes in proinflammatory and neurotrophic responses of microglia and astrocytes in a rat model of osmotic demyelination syndrome (2011)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Murase T, Ozaki N, Nagasaki H, Arima H, Murata Y, Sawada M, Oiso Y.
  • Journal Title: Glia Volume: 59(3) Pages: 452-62
  • Peer Reviewed
• [Journal Article] Minocycline Prevents Osmotic Demyelination Syndrome by Inhibiting the Activation of Microglia. (2010)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Nobuaki O, Nagasaki H, Arima H, Sawada M, Oiso Y
  • Journal Title: J Am Soc Nephrol Volume: 21(12) Pages: 2090-8
  • Peer Reviewed
• [Journal Article] Mechanisms Underlying Progressive Polyuria in Familial Neurohypophysial Diabetes Insipidus (2010)
  • Author(s): Arima H, Oiso Y
  • Journal Title: J Neuroendocrinol Volume: 22(7) Pages: 754-7
  • Peer Reviewed
• [Journal Article] Activation of vasopressin neurons leads to phenotype progression in a mouse model for familial neurohypophysial diabetes insipidus. (2010)
  • Author(s): Hiroi M, Morishita Y, Hayashi M, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Oiso Y,Arima H.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol Volume: 298(2) Pages: R486-93
  • Peer Reviewed
• [Presentation] マウスES細胞由来視床下部誘導系におけるバゾプレシン細胞の解析 (2012)
  • Author(s): 長崎 弘,福岡一貴,小谷 侑、須賀英隆、笹井芳樹、金子葉子、中島 顕, 大磯ユタカ、太田 明
  • Organizer: 第59回中部日本生理学会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター
  • Year and Date: 2012-11-17
• [Presentation] マウスES細胞由来視床下部細胞系による中枢性尿崩症治療 (2012)
  • Author(s): 福岡一貴、長崎 弘、須賀英隆、清田篤志、椙村益久、有馬寛、笹井芳樹、大磯ユタカ
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-18
• [Presentation] マウスES 細胞由来-視床下部培養系における視床下部ニューロンの発生 (2012)
  • Author(s): 長崎 弘大磯ユタカ
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-04-20
• [Presentation] 再生医療による中枢性尿崩症治療法の開発 (2012)
  • Author(s): 福岡一貴,長崎 弘、大磯ユタカ
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-04-20