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2012 Fiscal Year Final Research Report

The development of conditionally replicating adenovirus targeting cancer stem cell for innovative treatment for pediatric cancer

Research Project

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Project/Area Number 22390210
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKagoshima University

Principal Investigator

KOSAI Kenichiro  鹿児島大学, 大学院・医歯学総合研究科, 教授 (90258418)

Co-Investigator(Kenkyū-buntansha) MITSUI Kaoru  鹿児島大学, 大学院・医歯学総合研究科, 講師 (40324975)
IRIE Rie  鹿児島大学, 大学院・医歯学総合研究科, 助教 (90381178)
Co-Investigator(Renkei-kenkyūsha) KONDO Toru  北海道大学, 遺伝子病制御研究所, 教授 (30270573)
Project Period (FY) 2010 – 2012
Keywords小児腫瘍 / 遺伝子治療 / がん幹細胞 / アデノウイルスベクター / 増殖型ウイルス
Research Abstract

Although pediatric carcinomas have differentclinical and biological features from those of adult carcinomas, gene therapy for pediatric carcinoma has not been well developed. The present study aims to develop m-CRAs (conditionally replicating adenovirus targeting with multiple tumor-specific factors) that specifically treat cancer stem cells, leading to innovative therapy against pediatric carcinoma.
First, we have established the methods to enrich and purify cancer stem cells in soft tissue sarcoma, giloblastoma, hepatoblastoma et al.
Second, we revealed that our original m-CRAs efficiently treat cancer stem cells in such pediatric carcinoma.

  • Research Products

    (28 results)

All 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (16 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Alterations of Gene Expression and Glutamate Clearance in Astrocytes Derived from an MeCP2-null Mouse Model of Rett Syndrome2012

    • Author(s)
      Okabe Y, Takahashi T, Mitsumasu C, Kosai K, MD, Tanaka E, Matsuishi T
    • Journal Title

      PLoS ONE

      Volume: 7(4) Pages: e35354

    • DOI

      DOI:10.1371/journal.pone.0035354.

    • Peer Reviewed
  • [Journal Article] FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure.2012

    • Author(s)
      Hino S, Sakamoto A, Nagaoka K, Anan K, Wang Y, Mimasu S, Umehara T, Yokoyama S, Kosai K, and Nakao M
    • Journal Title

      Nat. Commun

      Volume: 27;3 Pages: 758

    • DOI

      DOI:10.1038/ncomms1755 (Open journal)

    • Peer Reviewed
  • [Journal Article] Targeting CD9 produces stimulus-independent antiangiogenic effects predominantly in activated endothelial cells during angiogenesis: A novel antiangiogenic therapy.2011

    • Author(s)
      Kamisasanuki T, Wang Y, Tokushige S, Terasaki H, Khai NC, Wang Y, Sakamoto T, Kosai K
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 16;413(1) Pages: 128-35

    • DOI

      DOI:10.1016/j.bbrc.2011.08.068.

    • Peer Reviewed
  • [Journal Article] Assessment of an altered E1B promoter on the specificity and potency of triple-regulated conditionally replicating adenoviruses (CRA) Implications for the generation of ideal m-CRAs.2011

    • Author(s)
      Horikawa Y, Wang Y, Nagano S, Kamizono J, Ikeda M, Komiya S, Kosai K
    • Journal Title

      Cancer Gene Ther

      Volume: 18(10) Pages: 724-33

    • DOI

      DOI:10.1038/cgt.2011.44.

    • Peer Reviewed
  • [Journal Article] Recombinant soluble form of HB-EGF protein therapy drastically inhibits Fas-mediated fulminant hepatic failure: Implications in clinical application2011

    • Author(s)
      Khai NC, Sakamoto K, Takamatsu H, Matsufuji H, Kosai K
    • Journal Title

      Hepatol Res

      Volume: 41 Pages: 594-596

    • DOI

      DOI:10.1111/j.1872-034X.2011.00805.x.

    • Peer Reviewed
  • [Journal Article] Therapeutic effects of vitamin A on experimental cholestatic rats with hepatic fibrosis.2011

    • Author(s)
      Murakami KI, Kaji T, Shimono R, Hyashida Y, Matsufuji H, Tsuyama S, Maezono R, Kosai K, Takamatsu H.
    • Journal Title

      Pediatr Surg Int

      Volume: 27(8) Pages: 863-70

    • DOI

      DOI:10.1007/s00383-011-2853-0

    • Peer Reviewed
  • [Journal Article] Neural Development of Methyl-CpG-Binding Protein 2 Null Embryonic Stem Cells: A System for Studying Rett Syndrome.2011

    • Author(s)
      Okabe Y, Kusanaga A, Takahashi T, Mitsumasu C, Murai Y, Tanaka E, Higashi H, Matsuishi T, Kosai K
    • Journal Title

      Brain Res

      Volume: 1360(11) Pages: 17-27

    • DOI

      DOI:10.1016/j.brainres.2010.08.090.

    • Peer Reviewed
  • [Journal Article] Anti-Fas Gene Therapy Prevents Doxorubicin-induced Acute Cardiotoxicity through Mechanisms Independent of Apoptosis2010

    • Author(s)
      Miyata S, Takemura G, Kosai K, Takahashi T, Esaki M, Li L, Kanamori H, Maruyama R, Goto K, Tsujimoto A, Takeyama T, Kawaguchi T, Ohno T, Nishigaki K, Fujiwara T, Fujiwara H, Minatoguchi S
    • Journal Title

      Am J Pathol

      Volume: 176(2) Pages: 687-98

    • DOI

      DOI:10.2353/ajpath.2010.090222

    • Peer Reviewed
  • [Journal Article] Leptin gene therapy in the fight against diabetes2010

    • Author(s)
      Wang Y, Asakawa A, Inui A, Kosai K
    • Journal Title

      Leptin gene therapy in the fight against diabetes

      Volume: 10(10) Pages: 1405-14

    • DOI

      DOI:10.1517/14712598.2010.512286

    • Peer Reviewed
  • [Presentation] Development of conditionally replicating adenovirus specifically targeting and/or efficiently treating cancer stem cells.2012

    • Author(s)
      Ken-ichiro Kosai
    • Organizer
      (国内、口演[English])第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      20120919-21
  • [Presentation] Survivin-Responsive Conditionally Replicating Adenovirus Efficiently Treats Rhabdomyosarcoma-Initiating Cells2012

    • Author(s)
      Kiyonori Tanoue, Yuqing Wang, Takao Setoguchi, Setsuro Komiya, Shoji Natusgoe, Ken-ichiro Kosai.
    • Organizer
      国内、口演[Japanese])第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      20120919-21
  • [Presentation] ヒトES/iPS細胞での再生医療の課題を克服する独自のアデノウイルスベクターと発現技術の開発2012

    • Author(s)
      小戝健一郎、三井薫、王宇清、高橋知之
    • Organizer
      (国内・パネルディスカッション)第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20120612-14
  • [Presentation] 独自開発の増殖型アデノウイルスベクターで癌幹細胞は効果的に治療できる2012

    • Author(s)
      田上聖徳、王宇清、池田美奈子、三井薫、瀬戸口啓夫、小宮節郎、夏越祥次、小戝健一郎
    • Organizer
      (国内・ポスター)第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20120612-14
  • [Presentation] 増殖型アデノウイルスベクターを用いた安全なヒトES/iPS細胞治療の開発2012

    • Author(s)
      三井薫、井手佳菜子、高山明子、小戝健一郎
    • Organizer
      (国内・口演)第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20120612-14
  • [Presentation] 癌の遺伝子異常を標的とした診断と治療の可能性2012

    • Author(s)
      小戝健一郎
    • Organizer
      (国内・特別講演)第175回日本医学放射線学会九州地方会
    • Place of Presentation
      鹿児島
    • Year and Date
      20120609-10
  • [Presentation] Efficient treatment of Rhabdomyosarcoma-initiating cells by Survivin-responsive conditionally replicating adenovirus : Promising m-CRA strategy for treating cancer stem cells2012

    • Author(s)
      Tanoue K, Wang Y, Ikeda M, Mitsui K, Setoguchi T, Komiya S, Natsugoe S, Kosai K.
    • Organizer
      (国際・ポスター)The American Society of Gene Therapy's 15h Annual Meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      20120516-19
  • [Presentation] HB-EGF/HGF HEPATIC GENE THERAPY FOR BILE DUCT LIGATED CHOLESTATIC LIVER INJURY IN MICE: THEIR DIFFERENT AND/OR SYNERGIC THERAPEUTIC EFFECTS2011

    • Author(s)
      Sakamoto K, Khai NC, Wang Y, Maezono R, Tanoue K, Matsufuji H, Hideo T, Kosai K
    • Organizer
      (国内・口演)第17回日本遺伝子治療学会
    • Place of Presentation
      福岡
    • Year and Date
      20110715-17
  • [Presentation] ASSESSMENT OF AN ALTERED E1B PROMOTER ON THE SPECIFICITY AND POTENCY OF TRIPLE-REGULATED CONDITIONALLY REPLICATING ADENOVIRUSES (CRA): A NEW INSIGHT TO GENERATE IDEAL m-CRAS.2011

    • Author(s)
      Kosai K, Horikawa Y, Wang Y, Nagano S, Kamizono J, Ikeda M, Komiya S
    • Organizer
      (国内・口演)第17回日本遺伝子治療学会
    • Place of Presentation
      福岡
    • Year and Date
      20110715-17
  • [Presentation] HB-EGF/HGF Hepatic Gene Therapy Effectively Inhibits Bile Duct Ligated Cholestatic Liver Injury In Mice InTheir Different Therapeutic Modes2011

    • Author(s)
      Kosai K, Sakamoto K, Khai NC, Wang Y, Maezono R, Tanoue K, Hideo T, Matsufuji H
    • Organizer
      The American Society of Gene Therapy's 14h Annual Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      20110515-22
  • [Presentation] RTT モデルES細胞由来神経細胞の電気生理学的解析2011

    • Author(s)
      岡部恭典、久佐賀晃、高橋知之、光益千秋、村井恵良、東英穂、松下豊次郎、小戝健一郎、田中永一郎
    • Organizer
      (国内・シンポジウム)第88回日本生理学会大会
    • Place of Presentation
      横浜
    • Year and Date
      20110328-30
  • [Presentation] MeCP2遺伝子欠損ES細胞のin vitro 文化誘導法によるRett症候群/神経発生の研究システム2011

    • Author(s)
      小戝健一郎、岡部共典、久佐賀晃、高橋知之、光益千秋、村井恵良、田中永一郎、松石豊次郎
    • Organizer
      (国内・口演)第10回日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      20110301-02
  • [Presentation] 胆道閉塞性肝疾患へのHB-EGF生体内再生医療2011

    • Author(s)
      坂本浩一、Ngin Cin Khai、王宇清、前薗理恵、高松英夫、松藤凡、小戝健一郎
    • Organizer
      (国内・口演)第10回日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      20110301-02
  • [Presentation] 膜蛋白CD9の標的治療はGeneralに作用する新規血管新生阻害剤となる2011

    • Author(s)
      寺崎寛人、上笹貫太郎、徳重沙織、Ngin Cin Khai、Wang Yuqing、坂本泰二、小戝健一郎
    • Organizer
      (国内・口演)第10回日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      20110301-02
  • [Presentation] Improved survivin-responsive m-CRA for efficient and safer cancer therapy: A new rule of E1B promoter replacement.2010

    • Author(s)
      Yoshiharu Horikawa, Ken-ichiro Kosai
    • Organizer
      国内・ポスター発表)第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      20100922-24
  • [Presentation] 膜蛋白CD9標的RNAiはGeneralに作用する新たな血管新生阻害剤となる2010

    • Author(s)
      上笹貫太郎、徳重沙織、坂本泰二、小戝健一郎
    • Organizer
      (国内・ポスター発表)第114回日本眼科学会総会
    • Year and Date
      20100415-18
  • [Remarks]

    • URL

      http://www.kufm.kagoshima-u.ac.jp/anatomy2/

  • [Patent(Industrial Property Rights)] 癌幹細胞を標的とするウイルスベクター2012

    • Inventor(s)
      小戝健一郎、王宇清
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Number
      特許PCT/JP2012/002031
    • Filing Date
      2012-03-23
    • Overseas
  • [Patent(Industrial Property Rights)] サービビン(Survivin)プロモーターを含む増殖型ベクターを有効成分とする医薬2012

    • Inventor(s)
      小戝健一郎、神囿純一
    • Industrial Property Rights Holder
      小戝健一郎
    • Industrial Property Number
      特許US 8,142,770 B2
    • Acquisition Date
      2012-03-27
    • Overseas

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Published: 2014-08-29   Modified: 2015-11-10  

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