Prevention of early loss of transplanted islets by targeting Na+/Ca2+exchanger of donor islets prior to transplantation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390251
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Fukuoka University
• Principal Investigator: YASUNAMI Yohichi 福岡大学, 医学部, 教授 (00166521)
• Co-Investigator(Kenkyū-buntansha): IWAMOTO Takahiro 福岡大学, 医学部, 教授 (20300973)
• Co-Investigator(Renkei-kenkyūsha): TANIGUCHI Masaru 独立行政法人理化学研究所, 免疫制御研究グループ, グループディレクター (80110310)
• Project Period (FY): 2010 – 2012
• Keywords: 移植外科学 / トランスポーター

Research Abstract

A major obstacle facing clinical islet transplantation is the low efficiency of this procedure due to innate immune rejection of transplanted islets. In the present study, we demonstrate that the innate immune rejection of transplanted islets is triggered by injury of islet cells caused by Ca2+ influx into 〓 cells via Na+/Ca2+ exchanger (NCX) and that innate immune rejection can be prevented by the treatment of donor islets with NCX inhibitor prior to transplantation.

Research Products

• [Journal Article] Prevention of High-Mobility Group Box 1-Mediated Early Loss of Transplanted Mouse Islets in the Liver by Antithrombin III. (2012)
  • Author(s): Kojima D, Mera T, Nishinakamura H, Itoh T, Ogata T, Matsuoka N, Kodama S, Yasunami Y.
  • Journal Title: Transplantation. Volume: 93(10) Pages: 983-8
  • Peer Reviewed
• [Journal Article] Pretreatment of donor islets with the Na+/Ca2+ exchanger inhibitor improves the efficiency of islet transplantation.
  • Author(s): Mera T, Itoh T, Kita S, Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y.
  • Journal Title: Am J Transplant. Volume: (In press)
  • Peer Reviewed
• [Presentation] 単離ドナー膵島のNa+/Ca2+交換体を標的にした移植前治療による肝内移植早期膵島障害の新規制御法 (2013)
  • Author(s): 米良利之,伊東威,喜多紗斗美,小島大望,西中村瞳,永石美晴,小方貴子,金城亜哉,岩本隆宏,小玉正太,安波洋一
  • Organizer: 第40回日本膵・膵島移植研究会
  • Place of Presentation: 高松
  • Year and Date: 2013-03-02
• [Presentation] Inhibitory effect of thrombomodulin on HMGB1-stimulated IFN-γ production of hepatic NKT and Gr-1+ Cells, facilitating to prevent early loss of transplanted islets in the liver of mice. (2012)
  • Author(s): Kojima D, Nishinakamura H, Ogata T, Nagaishi M, Mera T, Itoh T, Matsuoka N, Kodama S, Yasunami Y.
  • Organizer: 24th International Congress of the Transplantation society
  • Place of Presentation: ドイツ、ベルリン
  • Year and Date: 2012-07-19
• [Presentation] トロンボモデュリンによる移植膵島早期膵島障害の制御 (2012)
  • Author(s): 小島大望、米良利之、西中村瞳、伊東威、松岡信秀、小玉正太、安波洋一
  • Organizer: 第39回日本膵・膵島移植研究会
  • Place of Presentation: 旭川
  • Year and Date: 2012-03-10
• [Presentation] 肝内移植早期膵島障害の機序と制御法 (2012)
  • Author(s): 安波洋一
  • Organizer: 第39回日本膵・膵島移植研究会
  • Place of Presentation: 旭川
  • Year and Date: 2012-03-09
• [Presentation] 移植後24時間以内に発現する肝内移植膵島障害、喪失の機序解明と新規制御法開発 (2011)
  • Author(s): 米良利之、小島大望、西中村瞳、伊東威、松岡信秀、小玉正太、安波洋一
  • Organizer: 第38回日本膵・膵島移植研究会
  • Place of Presentation: 奈良
  • Year and Date: 2011-03-05