Establishment of the novel mouse model of metastatic colorectalcancer and identification of the biomarker for tissue-specific metastasis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390257
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hiroshima University
• Principal Investigator: HINOI Takao 広島大学, 病院, 講師 (10444689)
• Co-Investigator(Renkei-kenkyūsha): OKAJIMA Masazumi 広島大学, 医歯薬保健学研究院, 教授 (90274068) ; SOTOMARU Yusuke 広島大学, 自然科学研究支援開発センター, 教授 (90309352) ; IKENOUE Tsuneo 東京大学, 医学部附属病院, 助教 (80396712) ; OUE Naohide 広島大学, 医歯薬保健学研究院, 講師 (60346484) ; MIYAMOTO Kazuaki 呉医療センター, 臨床研究部, 室長 (30332373)
• Project Period (FY): 2010 – 2012
• Keywords: 小腸大腸肛門外科学

Research Abstract

We generated novel mouse model with complex mutation of colorectal cancer-related gene. (1) The appropriate condition for tumor development was optimized. (2) Observation of the tumor by micro-endoscope was performed.(3)Effect of the specific medication on the tumor growth was analyzed. (4) Afterestablishment of the mouse model with K-ras mutation, comprehensive gene profiling analysis of K-ras signaling was performed and its target gene was identified. In conclusion, useful mouse model for the individualized medication of colorectal cancer was established.

Research Products

• [Journal Article] Preservation of peritoneal fibrinolysis owing to decreased transcription of plasminogen activator inhibitor-1 in peritoneal mesothelial cells suppresses postoperative adhesion formation in laparoscopic surgery. (2013)
  • Author(s): Shimomura M, Hinoi T, Ikeda S, Adachi T, Kawaguchi Y, Tokunaga M, Sasada T, Egi H, Tanabe K, Okajima M, Ohdan H.
  • Journal Title: Surgery. 2013 Mar Volume: 153(3) Pages: 344-56
  • DOI: DOI:10.1016/j.surg.2012.07.037.
  • Peer Reviewed
• [Journal Article] Primary lung cancer presenting with metastasis to the colon: a case report. (2012)
  • Author(s): Sakai H, Egi H, Hinoi T, Tokunaga M, Kawaguchi Y, Shinomura M, Adachi T, Arihiro K, Ohdan H.
  • Journal Title: World J Surg Oncol Volume: 10 Pages: 127
  • DOI: DOI:10.1186/1477-7819-10-127.
  • Peer Reviewed
• [Journal Article] Reg IV is a direct target of intestinal transcriptional factor CDX2 in gastric cancer. (2012)
  • Author(s): Naito Y, Oue N, Hinoi T, Sakamoto N, Sentani K, Ohdan H, Yanagihara K, Sasaki, H, Yasui W.
  • Journal Title: PLoS One Volume: 7(11) Pages: e47545
  • DOI: DOI:10.1371/journal.pone.0047545.
  • Peer Reviewed
• [Journal Article] Submucosal invasive micropapillary carcinoma of the colon with massive lymph node metastases: a case report (2012)
  • Author(s): Mukai S, Takakura Y, Egi H, Hinoi T, Saito Y, Tanimine N, Miguchi M, Adachi T, Shimomura M, Ohdan H.
  • Journal Title: Case Rep Oncol. Volume: 5(3) Pages: 608-15
  • DOI: DOI:10.1159/000345566.
  • Peer Reviewed
• [Journal Article] Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer (2012)
  • Author(s): Sakamoto N, Oue N, Sentani K, Anami K, Uraoka N, Naito Y, Oo HZ, Hinoi T, Ohdan H, Yanagihara K, Aoyagi K, Sasaki H, Yasui W.
  • Journal Title: Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer Volume: 103(9) Pages: 1744-50
  • DOI: DOI:10.1111/j.1349-7006.2012.02353.x.
  • Peer Reviewed
• [Journal Article] Identification of patients likely to benefit from metastasectomy in stage IV colorectal cancer. (2012)
  • Author(s): Shimomura M, Okajima M, Hinoi T, Egi H, Takakura Y, Kawaguchi Y, Tokunaga M, Adachi T, Tashiro H, Ohdan H.
  • Journal Title: Int J Colorectal Dis. Volume: 27(10) Pages: 1339-46
  • Peer Reviewed
• [Journal Article] Adequate lymph node examination is essential to ensure the prognostic value of the lymph node ratio in patients with stage III colorectal cancer. (2011)
  • Author(s): Shimomura M, Ikeda S, Takakura Y, Kawaguchi Y, Tokunaga M, Egi H, Hinoi T, Okajima M, Ohdan H
  • Journal Title: Surg Today Volume: 41(10) Pages: 1370-9
  • DOI: DOI:10.1007/s00595-010-4446-2
  • Peer Reviewed
• [Journal Article] External validation of two nomograms for predicting patient survival after hepatic resection for metastatic colorectal cancer. (2011)
  • Author(s): Takakura Y, Okajima M, Kanemitsu Y, Kuroda S, Egi H, Hinoi T, Tashiro H, Ohdan H.
  • Journal Title: World J Surg. Volume: 35(10) Pages: 2275-82
  • DOI: DOI:10.1007/s00268-011-1194-4.
  • Peer Reviewed
• [Journal Article] Malignant peripheral nerve sheath tumor arising from the greater omentum: case report. (2011)
  • Author(s): Miguchi M, Takakura Y, Egi H, Hinoi T, Adachi T, Kawaguchi Y, Shinomura M,Tokunaga M, Okajima M, Ohdan H.
  • Journal Title: World J Surg Oncol. Volume: 9 Pages: 33
  • DOI: DOI:10.1186/1477-7819-9-33
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of colorectal cancer with gastric phenotype: claudin-18 is associated with poor prognosis. (2010)
  • Author(s): Matsuda M, Sentani K, Noguchi T, Hinoi T, Okajima M, Matsusaki K, Sakamoto N, Anami K, Naito Y, Oue N, Yasui W.
  • Journal Title: Pathol Int. Volume: 60(10) Pages: 673-80
  • DOI: DOI:10.1111/j.1440-1827.2010.02587.x.
  • Peer Reviewed
• [Journal Article] CDX2 regulates multidrug resistance 1 gene expression in malignant intestinal epithelium. (2010)
  • Author(s): Takakura Y, Hinoi T, Oue N, Sasada T, Kawaguchi Y, Okajima M, Akyol A, Fearon ER, Yasui W, Ohdan H.
  • Journal Title: Cancer Res. Volume: 70(17) Pages: 6767-78
  • DOI: DOI:10.1158/0008-5472.CAN-09-4701.
  • Peer Reviewed
• [Journal Article] Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: expression of DSC2 in gastric cancer with intestinal phenotype (2010)
  • Author(s): Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Hinoi T, Okajima M, Graff JM, Yasui W.
  • Journal Title: J Pathol. Volume: 221(3) Pages: 275-84
  • DOI: DOI:10.1002/path.2717.
  • Peer Reviewed
• [Journal Article] Gastrointestinal stromal tumors of the small intestine in pediatric populations: a case report and literature review. (2010)
  • Author(s): Shimomura M, Ikeda S, Takakura Y, Kawaguchi Y, Tokunaga M, Takeda H, Sumitani D, Yoshimitsu M, Hinoi T, Okajima M, Ohdan H.
  • Journal Title: Pediatr Surg Int. Volume: 26(6) Pages: 649-54
  • DOI: DOI:10.1007/s00383-010-2596-3.
  • Peer Reviewed
• [Presentation] Oncogenic K-rasmutation associates with GLUT1 expression in mice and human colorectal cancer (2013)
  • Author(s): KawaguchiY, Hinoi T, Sasada T, Adachi T, Saito Y, Miguchi S, Niitsu H, Ohdan H
  • Organizer: AACR Annual Meeting 2013
  • Place of Presentation: Washington DC USA
  • Year and Date: 20130406-10
• [Presentation] ChlorinatedDrinking Water Alters Carcinogenesis in a Mouse Model of Colon Cancer (2012)
  • Author(s): Sasada T, Hinoi T, et al
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: Chicago IL USA
  • Year and Date: 20120331-0404
• [Presentation] ChlorinatedDrinking Water Alters Carcinogenesis in a Mouse Model of Colon Cancer (2011)
  • Author(s): Sasada T, Hinoi T, Oue H, Yasui W, et al
  • Organizer: 6thInternational Society of Gastroenterological Carcinogenesis (ISGC)
  • Place of Presentation: Houston TX USA
  • Year and Date: 20110106-08