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2012 Fiscal Year Final Research Report

Live observation of three dimensional hepatic tissue with microvascular networks.

Research Project

  • PDF
Project/Area Number 22390260
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYokohama City University

Principal Investigator

KOIKE Naoto  横浜市立大学, 医学研究科, 客員研究員 (50301081)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Hideki  横浜市立大学, 医学研究科, 教授 (70292555)
Project Period (FY) 2010 – 2012
Keywords血管 / 肝臓 / 組織再構築 / 再生医療
Research Abstract

We provide a proof-of-concept study to engineer human liver tissue with fully functional vascular networks. We established a stable 3D culture platform to vascularize h uman fetal liver cells combined with human umbilical vein endothelial and mesenchymal cell populations. In vitro vascularized fetal liver cells were intravitally monitored the dynamic cellular organizing process after implantation under transparency window (cranial window). Engineered human tissue shows multiple liver-specific features with several hepatic functions. By analysis of the resected transplanted tissues and further animal experiments, it was confirmed that the tissues possessed the nature of the mature human hepatic tissue. Our new techniques discovered here should facilitate not only for future clinical use, but also for industrial use such as drug testing.

  • Research Products

    (16 results)

All 2013 2012 2011 2010

All Journal Article (13 results) (of which Peer Reviewed: 10 results) Presentation (3 results)

  • [Journal Article] Vascularised and functional human liver tissue from an induced pluripotent stem cell-derived organ bud transplant2013

    • Author(s)
      Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zang RR, Ueno Y, Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguchi H
    • Journal Title

      Nature

    • Peer Reviewed
  • [Journal Article] The generation of pancreatic beta-cell spheroids in a simulated microgravity culture system2013

    • Author(s)
      Tanaka H, Tanaka S, Sekine K, Kita S, Okamura A, Takebe T, Zheng YW, Ueno Y, Tanaka J, Taniguchi H
    • Journal Title

      Biomaterials

      Volume: 34 Pages: 5785-5791

    • DOI

      DOI:10.1016/j.biomaterials.2013.04.003

    • Peer Reviewed
  • [Journal Article] Generation of functional human vascular network2012

    • Author(s)
      Takebe T, Koike N, Sekine K, Enomura M, Chiba Y, Ueno Y, Zheng YW, Taniguchi H:
    • Journal Title

      Transplant Proc

      Volume: 44 Pages: 1130-1133

    • DOI

      DOI:10.1016/j.transproceed.2012.03.039

    • Peer Reviewed
  • [Journal Article] Imaging Studies of Intraductal Tubulopapillary Neoplasms of the Pancreas: 2-Tone Duct Sign and Cork-of-Wine-Bottle Sign as Indicators of Intraductal Tumor Growth2012

    • Author(s)
      Motosugi U, Yamaguchi H, Furukawa T, Ichikawa T, Hatori T, Fujita I, Yamamoto M, Motoi F, Kanno A, Watanabe T, Koike N, Koyama I, Kobayashi J, Shimizu M
    • Journal Title

      J Comput Assist Tomogr

      Volume: 36 Pages: 710-717

    • DOI

      DOI:10.1097/RCT.0b013e31826d1fc8

    • Peer Reviewed
  • [Journal Article] Gd-EOB-DTPA-Enhanced MRI versus Extracellular Contrast Medium-Enhanced MRI in Differentiation of Metastatic from Benign Liver Lesions2012

    • Author(s)
      Koike N, Onaya H
    • Journal Title

      Ann Gastroenterol Hepatol

      Volume: 3 Pages: 57-62

    • URL

      http://www.agh.sanlucasmedical.com/Issue/1-2012

    • Peer Reviewed
  • [Journal Article] A novel concept of identifying precancerous cells to enhance anti-cancer therapies2012

    • Author(s)
      Zheng YW, Tsuchida T, Taniguchi H
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 19 Pages: 621-625

    • DOI

      DOI:10.1007/s00534-012-0546-2

  • [Journal Article] Self-organization of human hepatic organoid by recapitulating organogenesis in vitro2012

    • Author(s)
      Takebe T, Sekine K, Suzuki Y, Enomura M, Tanaka S, Ueno Y, Zheng YW, Taniguchi H
    • Journal Title

      Transplant Proc

      Volume: 44 Pages: 1018-1020

    • DOI

      DOI:10.1016/j.transproceed.2012.02.007

    • Peer Reviewed
  • [Journal Article] Basics and applications of stem cells in the pancreas2012

    • Author(s)
      Sekine K, Taniguchi H
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 19 Pages: 594-599

    • DOI

      DOI:10.1007/s00534-012-0545-3

  • [Journal Article] Characteristics of hepatic stem/progenitor cells in the fetal and adult liver2012

    • Author(s)
      Koike H, Taniguchi H
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 19 Pages: 587-593

    • DOI

      DOI:10.1007/s00534-012-0544-4

  • [Journal Article] Establishment of automated culture system for murine induced pluripotent stem cells2012

    • Author(s)
      Koike H, Kubota K, Sekine K, Takebe T, Ouchi R, Zheng YW, Ueno Y, Tanigawa N, Taniguchi H
    • Journal Title

      BMC Biotechnol

      Volume: 12 Pages: 81

    • DOI

      DOI:10.1186/1472-6750-12-81

    • Peer Reviewed
  • [Journal Article] Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor2011

    • Author(s)
      Ishikawa M, Sekine K, Okamura A, Zheng YW, Ueno Y, Koike N, Tanaka J, Taniguchi H
    • Journal Title

      J Biosci Bioeng

      Volume: 111 Pages: 711-718

    • DOI

      DOI:10.1016/j.jbiosc.2011.01.019

    • Peer Reviewed
  • [Journal Article] Evidence for mesenchymal-epithelial transition associated with mouse hepatic stem cell differentiation2011

    • Author(s)
      Li B, Zheng YW, Sano Y, Taniguchi H
    • Journal Title

      PLoS One

      Volume: 6 Pages: e17092

    • DOI

      DOI:10.1371/journal.pone.0017092

    • Peer Reviewed
  • [Journal Article] Reconstruction of human elastic cartilage by a CD44+ CD90+ stem cell in the ear perichondrium2011

    • Author(s)
      Kobayashi S, Takebe T, Inui M, Iwai S, Kan H, Zheng YW, Maegawa J, Taniguchi H
    • Journal Title

      ProcNatl Acad Sci U S A

      Volume: 108 Pages: 14479-14484

    • Peer Reviewed
  • [Presentation] 如何にして器官発生を模倣するのか?ー血管系を有するヒト肝組織創出法の開発ー2013

    • Author(s)
      谷口英樹
    • Organizer
      第12回日本再生医療学会総会(招待講演)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-23
  • [Presentation] Evaluation of drainage fluid amylase level after pancreatojejunostomy using our method in pancreatoduodenectomy2011

    • Author(s)
      Koike N, Ohshima Y, Arita S, Takeuchi T, Ohkohchi N
    • Organizer
      International Surgical Week
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-08-31
  • [Presentation] Creation of long lasting human hepatic tissue in vivo2010

    • Author(s)
      Koike N, Fujiwara R, Nakano A, Chiba T, Amiya T, Sekine K, Taniguchi H
    • Organizer
      25thAnniversary tumor course. Critical issues in tumor microenvironment, angiogenesis and metastasis
    • Place of Presentation
      Harvard University, Cambridge, USA
    • Year and Date
      2010-09-26

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Published: 2014-08-29   Modified: 2015-01-14  

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