Neuranagenesis factor and comprehensive gene analysis for spinal cord injury

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390287
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Fukui
• Principal Investigator: BABA Hisatoshi 福井大学, 医学部, 教授 (00165060)
• Co-Investigator(Kenkyū-buntansha): UCHIDA Kenzo 福井大学, 医学部, 准教授 (60273009)
• Co-Investigator(Renkei-kenkyūsha): FURUKAWA Shoei 岐阜薬科大学, 薬学部, 教授 (90159129)
• Project Period (FY): 2010 – 2012
• Keywords: 脊椎脊髄病学 / 脊髄神経再生

Research Abstract

The present study was designed to investigate the effect of cyclic tensile stresses on cultured spinal cord cells using the Flexercell Strain Unit and DNA microarray technology.Spinal cord cells were isolated for culture from 15-day Sprague-Dawley rat embryos. The application of cyclic tensile stress reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. Increasing either the strain or the strain rate independently was associated with significant decreases in spinal cord cell survival. There was no clear evidence of additive effects of strain level with strain rate. GO analysis identified 44 candidate genes which were significantly related to “apoptosis” and 17 genes related to “response to stimulus”. KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated by RT-PCR analysis. These data may prove useful, as the accurate knowledge of neuronal gene expression in response to cyclic tensile stress will help in the development of molecular-based therapies for spinal cord injury.

Research Products

• [Journal Article] Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice (2012)
  • Author(s): Guerrero AR, Uchida K, Nakajima H, Watanabe S, Nakamura M, Johnson WE, Baba H
  • Journal Title: J Neuroinflammation Volume: 27:9:40
  • Peer Reviewed
• [Journal Article] Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury (2012)
  • Author(s): Nakajima H, Uchida K, Guerrero AR, Watanabe S, Sugita D, Takeura N, Yoshida A, Long G, Wright KT, Johnson WE, Baba H
  • Journal Title: J Neurotrauma Volume: 29 Pages: 1614-1625
  • Peer Reviewed
• [Journal Article] High-mobility Group Box-1 and Its Receptors Contribute to Proinflammatory Response in the Acute Phase of Spinal Cord Injury in Rats (2011)
  • Author(s): Chen KB, Uchida K, Nakajima H, Yayama T, Hirai T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Zhu P, Baba H
  • Journal Title: Spine Volume: 36 Pages: 2122-2129
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor-alpha antagonist reduces apoptosis of neurons and oligodendroglia in rat spinal cord injury (2011)
  • Author(s): Chen KB, Uchida K, Nakajima H, Yayama T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Baba H
  • Journal Title: Spine Volume: 36 Pages: 1350-1358
  • Peer Reviewed
• [Journal Article] Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro (2010)
  • Author(s): Uchida K, Nakajima H, Hirai T, Yayama T, Chen KB, Kobayashi S, Roberts S, Johnson WE, Baba H.
  • Journal Title: BMC Neurosci Volume: 11 Pages: 84
  • Peer Reviewed
• [Journal Article] Targeted retrograde gene delivery of brain-derived neurotrophic factor suppresses apoptosis of neurons and oligodendroglia after spinal cord injury in rats (2010)
  • Author(s): Nakajima H, Uchida K, Yayama T, Kobayashi S, Guerrero AR, Furukawa S, Baba H
  • Journal Title: Spine Volume: 35 Pages: 497-504
  • Peer Reviewed
• [Presentation] Blockade of interleukin-6 signaling generates an alternative activating environment in the spinal cord after injury:Promoting a neuroprotective macrophage population with concurrent locomotive recovery in mice (2011)
  • Author(s): Guerrero AR, Uchida K, Nakajima H, Hirai T, Watanabe S, Baba H
  • Organizer: 39th Annual Meeting of The Cervical Spine Research Society(CSRS 2011)
  • Place of Presentation: Arizona(USA)
  • Year and Date: 2011-12-08
• [Presentation] Microarray analysis of cell death-associated genes in rat embryo cultured spinal cord cells exposed to cyclic tensile stresses in vitro (2011)
  • Author(s): Uchida K, Nakajima H, Hirai T, Watanabe S, Guerrero AR, Baba H.
  • Organizer: The 27th Annual Meeting of the CSRS-ES
  • Place of Presentation: Istanbul(Turkey)
  • Year and Date: 2011-06-08
• [Presentation] メカニカルストレスに対する培養脊髄ニューロンの MAPK キナーゼに関する遺伝子発現プロファイル (2011)
  • Author(s): 内田研造, 中嶋秀明, 彌山峰史, 杉田大輔, 渡邉修司, 馬場久敏
  • Organizer: 第84回日本整形外科学会学術総会
  • Place of Presentation: Web 開催
  • Year and Date: 2011-05-12
• [Presentation] Some neuroprotective mathods for spinal cord injury (2011)
  • Author(s): Baba H, Uchida K, Nakajima H, WatanabeS, Takeura N, Yayama T
  • Organizer: The 13rd Intedrnational Southern China Cardiology Congress
  • Place of Presentation: Guangzhou(China)
  • Year and Date: 2011-04-21
• [Presentation] Cell death-associated genes expression patterns using microarray analysis in rat cultured spinal cord cells exposed to cyclic tensile stresses in vitro (2010)
  • Author(s): Uchida K, Nakajima H, Hirai T, Yayama T, Sugita D, Kobayashi S, Baba H
  • Organizer: 7^<th>Combined Meeting of ORS
  • Place of Presentation: Kyoto (Japan)
  • Year and Date: 2010-10-17
• [Presentation] マイクロアレイを用いたメカニカルストレスに対する脊髄ニューロンの遺伝子発現解析 (2010)
  • Author(s): 内田研造, 中嶋秀明, 彌山峰史, 杉田大輔, 渡邉修司, 馬場久敏
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知
  • Year and Date: 2010-04-23