2013 Fiscal Year Final Research Report
Regulation system of differentiation from mesenchymal stem cells to bone, vascular, and fatty tissues by mechanical loading and unloading
| Project/Area Number |
22390295
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
SAKAI Akinori 産業医科大学, 医学部, 教授 (90248576)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 荷重 / 非荷重 / 骨芽細胞 / 血管内皮細胞 / 脂肪細胞 / 未分化間葉系幹細胞 / 骨髄細胞 / 副甲状腺ホルモン受容体 |
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| Research Abstract |
We performed studies using exercise loading mice and unloading mice due to tail suspension. We demonstrated that the signal through PTH/PTHrP receptors promoted osteogenic differentiation and suppressed adipogenic differentiation from mesenchyman stem cells under loading condition. This signal is necessary for promotion of periosteal bone formation and enlargement of cross-sectional cortical shell. The expressions of CD31 and its receptor CD38 decreased after unloading and recovered to the normal loading levels after reloading. Co-culture experiments of osteoblasts and CD38 positive cells revealed that vascular endothelial factors are necessary for osteogenic differentiation.
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