Development of glycan-targetted assay system for diagnosis of prostate cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 22390301
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Hirosaki University
• Principal Investigator: OHYAMA Chikara 弘前大学, 医学(系)研究科(研究院), 教授 (80282135)
• Co-Investigator(Kenkyū-buntansha): KAMIMURA Noritaka 弘前大学, 医学研究科, 准教授 (00281931) ; HABUCHI Tomonori 秋田大学, 医学系研究科, 教授 (00293861) ; TSUBOI Shigeru 弘前大学, 医学研究科, 研究員 (20526727) ; MORI Kazuyuki 弘前大学, 医学研究科, 助教 (40266903) ; KOIE Takuya 弘前大学, 医学部附属病院, 講師 (60321965)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 前立腺癌 / 前立腺特異抗原 / バイオマーカー / 糖鎖

Outline of Final Research Achievements

To develop an assay system to measure prostate specific antigen (PSA) with cancer-associated aberrant glycosylation and to compare the diagnostic accuracy with conventional PSA test and %free PSA.We previously identified prostate cancer (PCa)-associated aberrant glycosylation on PSA, that is, α2,3-linked sialylation as an additional terminal N-glycan on free PSA (S2,3PSA). In the present study, we developed a new assay system for measurement of S2,3PSA with magnetic microbead-based immunoassay. We used the magplex beads to measure serum S2,3PSA, employing anti-human free PSA monoclonal antibody (8A6) for coating beads and anti-α2,3-linked sialic acid monoclonal antibody (HYB4) for detection. Area under the curve (AUC) for detection of PCa with S2,3 PSA was 0.84 which was significantly higher than those with PSA (0.57) or %free PSA (0.61). (P<0.001) The novel assay system had significantly higher specificity than conventional PSA test.

Free Research Field

泌尿器科学