Gene cascades for starting development and generating totipotency

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390312
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: HAMATANI Toshio 慶應義塾大学, 医学部, 講師 (60265882)
• Co-Investigator(Kenkyū-buntansha): AKUTSU Hidenori 国立成育医療研究センター, 生殖・細胞医療研究部, 室長 (50347225) ; TSUMURA Hideki 国立成育医療研究センター, 実験動物管理室, 室長 (20180052) ; YAMADA Mitsutoshi 慶應義塾大学, 医学部, 助教 (40383864) ; OGAWA Seiji 国立成育医療研究センター, 生殖・細胞医療研究部, 研究員 (10573371)
• Co-Investigator(Renkei-kenkyūsha): UMEZAWA Akihiro 国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486)
• Project Period (FY): 2010 – 2012
• Keywords: 生殖医学 / 再生医学

Research Abstract

Zygotic genome activation (ZGA) leads to the totipotent state in preimplantation embryos. Genes that are specifically expressed at the ZGA stages would be expected to have important roles in establishing pluripotency or preimplantation development. This s

Research Products

• [Journal Article] Stability of genomic imprinting in human induced pluripotent stem cells. (2013)
  • Author(s): Hiura H, Toyoda M, Okae H, Sakurai M, Miyauchi N, Sato A, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Nishino K, Umezawa A, Arima T.
  • Journal Title: BMC Genet. Volume: 14(1) Pages: 32
  • Peer Reviewed
• [Journal Article] β-Catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells. (2013)
  • Author(s): Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, Yoshida K, Ichida JK, Yamada M, Hamatani T, Kuji N, Miyado K, Yoshimura Y, Umezawa A.
  • Journal Title: Plos One Volume: (in press)
  • Peer Reviewed
• [Journal Article] Podocalyxin is a glycoprotein ligand of the human pluripotent stem cell-specific probe rBC2LCN. (2013)
  • Author(s): Tateno H, Matsushima A, Hiemori K, Onuma Y, Ito Y, Hasehira K, Nishimura K, Ohtaka M, Takayasu S, Nakanishi M, Ikehara Y, Nakanishi M, Ohnuma K, Chan T, Toyoda M, Akutsu H, Umezawa A, Asashima M, Hirabayashi J.
  • Journal Title: Stem Cells Transl Med Volume: 2(4) Pages: 265-73
  • Peer Reviewed
• [Journal Article] A medium-chain fatty acid as an alternative energy source in mouse preimplantation development. (2012)
  • Author(s): Yamada M, Takanashi K, Hamatani T, Hirayama A, Akutsu H, Fukunaga T, Ogawa S, Sugawara K, Soga T, Umezawa T, Kuji N, Yoshimura Y, Tomita M.
  • Journal Title: Scientific Reports Volume: 2 Pages: 930
  • Peer Reviewed
• [Journal Article] Production of functional classical brown adipocytes from human pluripotent stem cells using specific hemopoietin cocktail without gene transfer. (2012)
  • Author(s): Nishio M, Yoneshiro T, Nakahara M, Suzuki S, Saeki K, Hasegawa M, Kawai Y, Akutsu H, Umezawa A, Yasuda K, Tobe K, Yuo A, Kubota K, Saito M, Saeki K.
  • Journal Title: Cell Metab Volume: 16(3) Pages: 394-406
  • Peer Reviewed
• [Journal Article] Investigating cellular identity and manipulating cell fate using induced pluripotent stem cells. (2012)
  • Author(s): Sugawara T, Nishino K, Umezawa A, Akutsu H.
  • Journal Title: Stem Cell Res Ther. Volume: 3(2) Pages: 8
  • Peer Reviewed
• [Journal Article] 【不妊と周産期医療】ART妊娠児の長期予後研究. (2012)
  • Author(s): 久慈直昭, 山田満稔, 井上治, 福永朝子, 小川誠司, 菅原かな, 奥村典子, 浜谷敏生, 吉村泰典.
  • Journal Title: 周産期医学 Volume: 42(8) Pages: 991-1000
• [Journal Article] Human spermatozoal RNAs. (2012)
  • Author(s): Hamatani T
  • Journal Title: Fertil Steril Volume: 97(2) Pages: 275-81
  • Peer Reviewed
• [Journal Article] Early senescence is not an inevitable fate of human-induced pluripotent stem-derived cells. (2011)
  • Author(s): Gokoh M, Nishio M, Nakamura N, Matsuyama S, Nakahara M, Suzuki S, Mitsumoto M, Akutsu H, Umezawa A, Yasuda K, Yuo A, Saeki K.
  • Journal Title: Cell Reprogram. Volume: 13(4) Pages: 361-70
  • Peer Reviewed
• [Journal Article] DNA methylation dynamics in human induced pluripotent stem cells over time. (2011)
  • Author(s): Nishino K, Toyoda M, Yamazaki-Inoue M, Fukawatase Y, Chikazawa E, Sakaguchi H, Akutsu H, Umezawa A.
  • Journal Title: PLoS Genet. Volume: 7(5) Pages: e1002085
  • Peer Reviewed
• [Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage. (2011)
  • Author(s): Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N.
  • Journal Title: BMC Dev Biol. Volume: 11 Pages: 22
  • Peer Reviewed
• [Journal Article] A distinct role for Pin1 in the induction and maintenance of pluripotency. (2011)
  • Author(s): Nishi M, Akutsu H, Masui S, Kondo A, Nagashima Y, Kimura H, Perrem K, Shigeri Y, Toyoda M, Okayama A, Hirano H, Umezawa A, Yamamoto N, Lee SW, Ryo A.
  • Journal Title: J Biol Chem. Volume: 286(13) Pages: 11593-603
  • Peer Reviewed
• [Journal Article] Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX. (2011)
  • Author(s): Inamura M, Kawabata K, Takayama K, Tashiro K, Sakurai F, Katayama K, Toyoda M, Akutsu H, Miyagawa Y, Okita H, Kiyokawa N, Umezawa A, Hayakawa T,Furue MK, Mizuguchi H.
  • Journal Title: Mol Ther. Volume: 19(2) Pages: 400-7
  • Peer Reviewed
• [Journal Article] Lectin microarray analysis of pluripotent and multipotent stem cells. (2011)
  • Author(s): Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.
  • Journal Title: Genes Cells. Volume: 16(1) Pages: 1-11
  • Peer Reviewed
• [Journal Article] β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion. (2011)
  • Author(s): Takezawa Y, Yoshida K, Miyado K, Sato M, Nakamura A, Kawano N, Sakakibara K, Kondo T, Harada Y, Ohnami N, Kanai S, Miyado M, Saito H, Takahashi Y, Akutsu H, Umezawa A.
  • Journal Title: Sci Rep Volume: 1 Pages: 68
  • Peer Reviewed
• [Journal Article] Spermatozoal RNA profiling towards a clinical evaluation of sperm quality. (2011)
  • Author(s): Hamatani T.
  • Journal Title: Reprod BioMed Online Volume: 22(2) Pages: 103-105
  • Peer Reviewed
• [Journal Article] Chemical inhibition of sulfation accelerates neural differentiation of mouse embryonic stem cells and human induced pluripotent stem cells. (2010)
  • Author(s): Sasaki N, Hirano T, Kobayashi K, Toyoda M, Miyakawa Y, Okita H, Kiyokawa N, Akutsu H, Umezawa A, Nishihara S
  • Journal Title: Biochem Biophys Res Commun Volume: 401(3) Pages: 480-6
  • Peer Reviewed
• [Journal Article] Defining hypo-methylated regions of stem cell-specific promoters in human iPS cells derived from extra-embryonic amnions and lung fibroblasts. (2010)
  • Author(s): Nishino K, Toyoda M, Yamazaki-Inoue M, Makino H, Fukawatase Y, Chikazawa E, Takahashi Y, Miyagawa Y, Okita H, Kiyokawa N, Akutsu H, Umezawa A.
  • Journal Title: PLoS One Volume: 5(9) Pages: e13017
  • Peer Reviewed
• [Journal Article] Acute stress may induce ovulation in women. (2010)
  • Author(s): Tarin JJ, Hamatani T, Cano A.
  • Journal Title: Reprod Biol Endocrinol. Volume: 26;8 Pages: 53
  • Peer Reviewed
• [Journal Article] Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. (2010)
  • Author(s): Stadtfeld M, Apostolou E, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K.
  • Journal Title: Nature Volume: 465(7295) Pages: 175-81
  • Peer Reviewed
• [Presentation] Metabolomic analysis of culture media by CE-TOF/MS suggests a medium-chain fatty acid as an alternative energy source of mouse preimplantation embryos (2012)
  • Author(s): Yamada M, Takanashi K, Hamatani T, Akutsu H, Fukunaga T, Ogawa S, Sugawara K, Okumura N, Chikazawa N, Kuji N, Umezawa A, Tomita M, Yoshimura Y.
  • Organizer: 28th annual meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Istanbul
  • Year and Date: 20120701-04
• [Presentation] シンポジウム:生殖内分泌における酸化ストレスとエイジング:マウス卵における酸化ストレスとエイジング (2012)
  • Author(s): 浜谷敏生
  • Organizer: 第17回日本生殖内分泌学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2012-12-08
• [Presentation] 着床前期胚発生に関わる新規遺伝子Zfpiの発現解析 (2012)
  • Author(s): 小川誠司, 浜谷敏生, 阿久津英憲, 山田満稔, 奥村典子, 菅原かな, 井上治, 福永朝子, 梅澤明弘, 久慈直昭, 青木大輔, 吉村泰典
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2012-04-13
• [Presentation] 着床前期初期から発現し多分化能細胞に特異的に発現する新規転写因子Kzpiの機能解析. (2012)
  • Author(s): 浜谷敏生, 山田満稔, 阿久津英憲, 小川誠司, 菅原かな, 奥村典子, 福永朝子, 井上治, 梅澤明弘, 久慈直昭, 青木大輔, 吉村泰典
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2012-04-13
• [Presentation] Identification of a novel gene encoding a high mobility group box protein and its specific expression and essential role during preimplantation development. (2011)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Kuji N, Aoki D, Yoshimura Y.
  • Organizer: The 67th Annual Meeting of the American Society for Reproductive Medicine (ASRM)
  • Place of Presentation: Orland, USA
  • Year and Date: 20111021-24
• [Presentation] シンポジウム:卵巣機能異常に起因する不妊治療の最前線:マウス卵の加齢メカニズム (2011)
  • Author(s): 浜谷敏生.
  • Organizer: 第56回日本生殖医学会学術講演会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-09
• [Presentation] Metabolomic analysis of culture media by CE-TOF/MS suggests a medium-chain fatty acid as an alternative energy source of mouse preimplantation embryos. (2011)
  • Author(s): Hamatani T
  • Organizer: The 2nd international symposium of CHA fertility Center on reproductive science
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-10-28
• [Presentation] マウス卵子の排卵後加齢における遺伝子発現と酸素消費量との関連. (2011)
  • Author(s): 福永朝子, 浜谷敏生, 山田満稔, 阿部宏之, 阿久津英憲, 井上治, 小川誠司, 菅原かな, 奥村典子, 久慈直昭, 青木大輔, 吉村泰典
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2011-08-30
• [Presentation] 着床前期および未分化維持に関わる新規C2H2-zinc finger遺伝子の解析 (2011)
  • Author(s): 近澤奈々, 浜谷敏生, 山田満稔, 阿久津英憲, 奥村典子, 小川誠司, 菅原かな, 井上治, 福永朝子, 久慈直昭, 吉村泰典
  • Organizer: 第52回日本哺乳動物卵子学会
  • Place of Presentation: 大田原
  • Year and Date: 2011-05-21
• [Presentation] Identification of a novel gene encoding a high mobility group box protein and its specific expression and essential role during preimplantation development. (2010)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Chikazawa N, Kuji N, Yoshimura Y, Umezawa A.
  • Organizer: The 26th Annual meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Rome, Italy
  • Year and Date: 20100628-30
• [Presentation] Identification of a novel gene encoding a high mobility group box protein and its specific expression and essential role during preimplantation development. (2010)
  • Author(s): Yamada M, Hamatani T, Akutsu H, Chikazawa N, Kuji N, Yoshimura Y, Umezawa A.
  • Organizer: The First SKLRB Symposia on Frontiers in Periimplantation Biology
  • Place of Presentation: the Beijing Friendship Hotel, Beijing
  • Year and Date: 20100508-12
• [Presentation] マウス卵子における加齢によるテロメア長短縮とテロメラーゼ活性の変化 (2010)
  • Author(s): 山田満稔,浜谷敏生,阿久津英憲,井上治,福永朝子,小川誠司,菅原かな,奥村典子,近澤奈々,浅田弘法,久慈直昭,吉村泰典.
  • Organizer: 第55回日本生殖医学会・学術講演会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-11
• [Presentation] 着床前期胚に特異的な発現が予想された新規zinc finger遺伝子の発現・機能解析 (2010)
  • Author(s): 近澤奈々,浜谷敏生,山田満稔,阿久津英憲,奥村典子,久慈直昭,吉村泰典
  • Organizer: 第55回日本生殖医学会・学術講演会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-11
• [Presentation] シンポジウム:発生・生殖生物学の最前線:着床前期に特異的に発現する新規遺伝子Hmgpiの発現および機能解析 (2010)
  • Author(s): 浜谷敏生
  • Organizer: 第51回日本組織細胞化学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-05
• [Presentation] 着床前期胚特異的新規遺伝子Hmgpiによる着床周辺期発生の制御機構 (2010)
  • Author(s): 山田満稔,浜谷敏生,阿久津英憲,小川誠司,奥村典子,持丸佳之,高野光子,浅田弘法,久慈直昭,青木大輔,梅澤明弘,吉村泰典
  • Organizer: 第51回日本哺乳動物卵子学会
  • Place of Presentation: 新潟
  • Year and Date: 2010-05-30
• [Book] 生殖卵巣学-基礎知識と臨床の進展(編集:石塚文平、鈴木秋悦).第2 章(9)卵巣の老化 (2011)
  • Author(s): 菅原かな、浜谷敏生
  • Publisher: 医歯薬出版,東京.
• [Remarks]
  • URL: http://www.obgy.med.keio.ac.jp/