Study on the survival strategies for periodontal bacteria in host cells and their role in the pathogenesis of periodontal diseases

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390342
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Okayama University
• Principal Investigator: OHARA Naoya 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70223930)
• Co-Investigator(Kenkyū-buntansha): NAKAYAMA Masaaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10579105) ; OHARA Naoko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (80301365)
• Project Period (FY): 2010 – 2012
• Keywords: 歯周病原細菌 / 細胞内寄生 / Porphyromonas / 嫌気性菌 / 感染

Research Abstract

Porphyromonas gingivalisis the major periodontal pathogens. 1) We identified P. gingivalis genes required for survival in the host cells using newly constructed transposon mutagenesis library of P. gingivalis. 2) We found the novel cellular events in host epithelial cells by challenging of P. gingivalis. Akt and its substrates were dephosphorylated by P. gingivalis infection. These results indicated that P. gingivalis-infection may affect cellular functions including apoptosis, glucose metabolism and cell development in host cells by inactivating the PI3K/AKt pathway.

Research Products

• [Presentation] P. gingivalisジンジパインによるPI3K/Akt経路の抑制機序 (2013)
  • Author(s): 中山真彰、井上哲圭、大原直也
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] P. gingivalisのECFシグマ因子PG1318 変異株はミューテーター形質を示す (2013)
  • Author(s): 菊池有一郎、柴山和子、国分栄二、大原直也、中山浩次、石原和幸
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
  • Year and Date: 20130318-20
• [Presentation] Porphyromonas gingivali によるAkt/GSK3 beta pathwayの制御 (2012)
  • Author(s): 中山真彰,井上哲圭,大原直也
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
  • Year and Date: 2012-09-16
• [Presentation] New aspect of signaling events in Porphyromonas gingivalispathogenesis (2012)
  • Author(s): 大原直也
  • Organizer: First International Conference on Porphyromonas gingivalisand related Bacterial Species
  • Place of Presentation: 長崎市
  • Year and Date: 2012-08-27
• [Presentation] 歯周病原細菌およびその病原性因子の全身疾患への影響 (2012)
  • Author(s): 大原直也
  • Organizer: 第60回日本化学療法学会学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-04-26
• [Presentation] Inhibition of RANK ligand- induced osteoclast differentiation by Porphyromonas gingivalis. (2011)
  • Author(s): Nakayama M, Nakayama K, Kobayashi K, Ohara N
  • Organizer: XIII International Congress of Bacteriology and Applied MicrobiologyXIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] Roles of the extracytoplasmic function sigma factors in Porphyromonas gingivalis. (2011)
  • Author(s): Kikuchi Y, Onozawa S, Kiso A, Miyashita M, Ueda O, Hirai K, Shibata Y, Ohara N, Nakayama K, Fujimura S
  • Organizer: XIII International Congress of Bacteriology and Applied MicrobiologyXIII International Congress of Bacteriology and Applied Microbiology, International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 20110906-10
• [Presentation] 歯周病原細菌Porphyromonas gingivalisの新たな病原性に関する研究.特別講演 (2010)
  • Author(s): 大原直也
  • Organizer: 第31回岡山歯学会総会・学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-09-26