Understanding of non-canonical Wnt signaling network in bone and cartilage formation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390348
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Osaka University
• Principal Investigator: NISHIMURA Riko 大阪大学, 大学院・歯学研究科, 教授 (60294112)
• Co-Investigator(Renkei-kenkyūsha): HATA Kenji 大阪大学, 大学院・歯学研究科, 准教授 (80444496)
• Project Period (FY): 2010 – 2012
• Keywords: Wnt / 骨芽細胞 / 軟骨細胞 / 転写因子

Research Abstract

To understand the role of non-canonical Wnt including Wnt5a, weinvestigated the phenotype of Wnt5a null mice. The Wnt5a null mice showed severe impairment of skeletal development, especially in cartilage formation. We have attempted to identify the target of Wnt5a, and found that homeobox gene would be involved in Wnt5a-regulated endochondral ossification.

Research Products

• [Journal Article] Arid5a controls IL-6 mRNA stability, which contributes to elevation of IL-6 level in vivo. (2013)
  • Author(s): Masuda K, Ripley B, Nishimura R, Mino T, Takeuchi O, Kiyonari H, Shioi G, Kishimoto T
  • Journal Title: Proc Natl Acad Sci USA Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Regulation of endochondral ossification by transcription factors. (2013)
  • Author(s): Nishimura R, Hata K, Ono K, Takashima R, Yoshida M, YonedaT
  • Journal Title: J Oral Bioscience Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Retinoic acid regulates commitment of undifferentiated mesenchymal stem cells into osteoblasts and adipocytes. (2013)
  • Author(s): Hisada K, Hata K, Ichida F, Matsubara T, Orimo H, Nakano T, Yatani H, Nishimura R, Yoneda T
  • Journal Title: J Bone Miner Metab Volume: 31巻 Pages: 53-63
  • Peer Reviewed
• [Journal Article] Osterix Regulates Calcification and Degradation of Chondrogenic Matrices Through Matrix Metalloproteinase (MMP13) Expression in Association with Transcription Factor Runx2 During Endochondral Ossification. (2012)
  • Author(s): Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, ShioiG, YamaguchiA, Tsumaki N, Akiyama H, Yoneda T
  • Journal Title: J Biol Chem Volume: 287巻 Pages: 33179-33190
  • Peer Reviewed
• [Journal Article] Regulation of bone and cartilage development by network between BMP signaling and transcription factors. (2012)
  • Author(s): Nishimura R, Hata K, Mastubara T, Wakabayashi M, Yoneda T
  • Journal Title: J Biochem Volume: 151巻 Pages: 247-254
  • Peer Reviewed
• [Journal Article] Regulation of endochondral ossification by transcription factors. (2012)
  • Author(s): Nishimura R, Hata K, Ono K, Amano K, Takigawa Y, Wakabayashi M, Takashima R, Yoneda T
  • Journal Title: Frontiers in Biosci-Landmar Volume: 17巻 Pages: 2657-2666
  • Peer Reviewed
• [Journal Article] 内軟骨性骨形成過程における転写因子の役割と機能制御 (2012)
  • Author(s): 西村 理行、波多 賢二、高島利加子、吉田倫子
  • Journal Title: Clinical Calcium Volume: 22巻 Pages: 643-652
• [Journal Article] Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription (2011)
  • Author(s): Amano K, Hata K, MuramatsuS, Wakabayashi M, Takigawa Y, Ono K, Nakanishi M, Takashima R, Kogo M, Matsuda A, NishimuraR, Yoneda T
  • Journal Title: Mol Biol Cell Volume: 22巻 Pages: 1300-1311
  • Peer Reviewed
• [Journal Article] Distinct mechanisms are responsible for osteopenia and growth retardation in OASIS-deficient mice (2011)
  • Author(s): Murakami T, Hino S, Nishimura R, Yoneda T, Wanaka A, Imaizumi K
  • Journal Title: Bone Volume: 48巻 Pages: 514-523
  • Peer Reviewed
• [Journal Article] 骨芽細胞の分化制御機構 (2011)
  • Author(s): 西村理行
  • Journal Title: Clinical Calcium Volume: 21巻 Pages: 103-112
• [Journal Article] A transcription factor Znf219 regulates chondrocyte differentiation by assembling transcription factory with Sox9 (2010)
  • Author(s): Takigawa Y, Hata K, Muramatsu S, Amano K, Ono K, Wakabayashi M, Matsuda A, Takada K, Nishimura R, Yoneda T
  • Journal Title: J Cell Sci Volume: 123巻 Pages: 3780-3788
  • Peer Reviewed
• [Presentation] 転写因子 Arid5bは Sox9標的遺伝子プロモーター領域のヒストン脱メチル化を介して軟骨細胞分化を促進する (2013)
  • Author(s): 波多 賢二、高島利加子、Whitson RH、西村理行、米田俊之
  • Organizer: 軟骨代謝学会シンポジウム、第26回日本軟骨代謝学会
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2013-03-01
• [Presentation] 骨格制御過程における転写因子の機能的役割 (2012)
  • Author(s): 西村理行
  • Organizer: 第24回骨代謝セミナー
  • Place of Presentation: フォーシーズンズホテル椿山荘
  • Year and Date: 2012-12-21
• [Presentation] Arid5a is an IL-6 mRNA stability protein. Chlorpromazine mediates its inhibitory effect on IL-6 production in macrophages through inhibition of Arid5a expression (2012)
  • Author(s): Masuda K, Barry R, Nishimura R, Takeuchi O, Takashi M, Daron S, Kishimoto T
  • Organizer: 第41回免疫学会学術集会シンポジウム
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-12-07
• [Presentation] 骨形成過程における転写因子の制御メカニズム (2012)
  • Author(s): 西村理行
  • Organizer: 第28回長崎骨粗鬆症研究会
  • Place of Presentation: 長崎県医師会館
  • Year and Date: 2012-10-31
• [Presentation] The Transcription Factor FoxC1 Regulates Chondrogenesis Together with Gli2 through Induction of PTHrP (2012)
  • Author(s): Yoshida M, Hata K, Takashima R, Iseki S, Takano-Yamamoto T, Nishimura R, Yoneda T
  • Organizer: ASBMR Annual Meeting
  • Place of Presentation: Minneapolis Convention Center
  • Year and Date: 2012-10-12
• [Presentation] 転写因子 Arid5bは Sox9標的遺伝子プロモーター領域のヒストン脱メチル化を介して軟骨細胞分化を制御する (2012)
  • Author(s): 波多 賢二、西村 理行
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 奥羽大学
  • Year and Date: 2012-09-16
• [Presentation] The transcription factor FoxC1 regulates chondrogenesis together with Gli2 through induction ofPTHrP (2012)
  • Author(s): Yoshida M, Hata K, Takashima R, Iseki S, Takano-Yamamoto T, Nishimura R, Yoneda T
  • Organizer: Australian & New Zealand Bone & Mineral Society 22nd Annual Scientific Meeting in conjunction with 1st Asia-Pacific Bone andMineral Research Meeting
  • Place of Presentation: Pan Pacific Hotel Perth Western Australia
  • Year and Date: 2012-09-04
• [Presentation] 軟骨細胞分化のエピゲノム (2012)
  • Author(s): 波多 賢二、高島 利加子、西村 理行、米田 俊之
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2012-07-19
• [Presentation] 転写因子 FoxC1は PTHrPの発現を介して内軟骨性骨形成を制御する (2012)
  • Author(s): 吉田 倫子、波多 賢二、高島利加子、井関 祥子、山本照子、西村 理行、米田 俊之
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2012-07-19
• [Presentation] 骨格形成過程における転写因子ネットワークの制御機構 (2012)
  • Author(s): 西村理行
  • Organizer: 第66回口腔科学会教育研修会
  • Place of Presentation: リーガロイヤルホテル広島
  • Year and Date: 2012-05-16
• [Presentation] 骨格形成過程における転写因子の制御機構とその役割 (2012)
  • Author(s): 西村理行
  • Organizer: 第20回 Club for Cartilage and Bone カンファレンス
  • Place of Presentation: 天王寺都ホテル
  • Year and Date: 2012-01-26
• [Presentation] 骨格形成過程における転写制御ネットワークシステムの分子機構 (2011)
  • Author(s): 西村理行
  • Organizer: 第53回歯科基礎医学会学術大会サテライトシンポジウム
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2011-09-30
• [Presentation] The transcription factor Arid5B is a novel partner of Sox9 in endochondral bone formation (2011)
  • Author(s): Takashima R, Hata K, Wakabayashi M, Nakanishi M, Ono K, Amano K, Whitson R, Maeda Y, Nishimura R, Yoneda T
  • Organizer: ASBMR 2011 Annual Meeting
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2011-09-18
• [Presentation] The transcription factor Arid5b modulates endochondral bone formation in cooperation withSox9 (2011)
  • Author(s): Takashima R, Hata K, Wakabayashi M, Nakanishi M, Ono K, Amano K, Whitson R, Maeda Y, Nishimura R, Yoneda T
  • Organizer: Joint 2nd Asia-Pacific Osteoporosis and Bone Meeting & ANZBMS Annual Meeting
  • Place of Presentation: Australia, Gold Coast Convention center
  • Year and Date: 2011-09-05
• [Presentation] Arid5b はSox9の転写機能を促進することにより軟骨細胞分化を制御する (2011)
  • Author(s): 高島理加子、波多賢二、若林真、小野孝一郎、天野克比古、中西雅子、前田芳信、西村理行、米田俊之
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-07-29
• [Presentation] Regulation of bone development by Sox9 and Osterix (2010)
  • Author(s): Nishimura R
  • Organizer: 7th BoneBiology Forum
  • Place of Presentation: 富士研修センター
  • Year and Date: 2010-08-21
• [Book] 血液・尿化学検査日本臨牀増刊号オステオポンチン、オステオネクチン (2010)
  • Author(s): 西村理行
  • Total Pages: 233-235
  • Publisher: 日本臨牀社