2012 Fiscal Year Final Research Report
Analysis of osteoclast niche regulated by Wnt signals.
| Project/Area Number |
22390351
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBOYASHI Yasuhiro 松本歯科大学, 総合歯科医学研究所, 准教授 (20264252)
MIZOGUCHI Toshihide 松本歯科大学, 総合歯科医学研究所, 講師 (90329475)
NINOMIYA Tadashi 松本歯科大学, 総合歯科医学研究所, 講師 (00360222)
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| Co-Investigator(Renkei-kenkyūsha) |
UDAGAWA Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801)
NAKAMURA Hiroaki 松本歯科大学, 歯学部, 教授 (50227930)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 口腔生化学 |
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| Research Abstract |
We examined the role of Wnt signaling for the formation of osteoclast niche, and the results as follows. (1) The osteoclast niche was analyzed and characteristics of osteoclast precursors (quiescent osteoclast precursors, QOP) were clarified. (2) The role of noncanonical Wnt signaling in osteoclastogenesis was examined. Noncanonical Wnt signals (Wnt5a-Ror2 signals) were shown to play important roles in osteoclastogenesis. (3) Wnt5a-Ror2 signals enhanced RANKexpression in QOPs. Canonical Wnt signals did not appear to play a role in osteoclastogenesis. (4) GeneChip analysis was performed on QOPs to identify specific markers of QOPs. But we failed to isolate specific markers of QOPs. However, we found that osteoclasts specifically express Wnt5a.(5) IL-34 was identified as an important cytokine for generation of QOP in hematopoietic tissues. (6) Alveolar bone loss was examined in OPG-deficient (OPG?/?) mice and RANKL-overexpressing transgenic mice. OPG?/? mice were shown to be a use l model of periodontitis. (7) Administration of soluble form of Ror2 into collagen-induced arthritis mice effectively suppressed bone destruction.
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Research Products
(33 results)
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[Journal Article] Spleen serves as a reservoir of osteoclast precursors through vitamin D-induced IL-34 expression in CSF-1op/op mice.2012
Author(s)
Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato S, DeLuca HF, Suda T, Udagawa N, Takahashi N
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Journal Title
Proc Natl Acad Sci UAS
Volume: 109
Pages: 10006-10011
DOI
Peer Reviewed
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[Journal Article] Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis.2012
Author(s)
Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N
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Journal Title
Nature Med
Volume: 18
Pages: 405-412
DOI
Peer Reviewed
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[Journal Article] Daily administration of Eldecalcitol (ED-71), an active vitamin D analog, increases bone mineral density by suppressing RANKL expression in mouse trabecular bone.2012
Author(s)
Harada S, Mizoguchi T, Kobayashi Y, Nakamichi Y, Takeda S, Sakai S, Takahashi F, Saito H, Yasuda H, Udagawa N, Suda T, Takahashi N
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Journal Title
J Bone Mineral Res
Volume: 27
Pages: 461-473
DOI
Peer Reviewed
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[Journal Article] Polarized osteoclasts put marks of tartrate-resistant acid phosphataseon dentin slices -A simple method for identifying polarized osteoclasts-2011
Author(s)
Nakayama T, Mizoguchi T, Uehara S, Yamashita T, Kawahara I, Kobayashi Y, Moriyama Y, Kurihara S, Sahara N, Ozawa H, Udagawa N, Takahashi N
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Journal Title
Bone
Volume: 49(6)
Pages: 1331-1339
DOI
Peer Reviewed
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[Journal Article] Lineage-committed osteoclast precursors circulate in blood and settle down into bone.2011
Author(s)
Muto A, Mizoguchi T, Udagawa N, Ito S, Kawahara I, Abiko Y, Arai A, Harada S, Kobayashi Y, Nakamichi Y, Penninger JM, Noguchi T, Takahashi N
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Journal Title
J Bone Mineral Res
Volume: 26
Pages: 2978-2990
DOI
Peer Reviewed
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