2012 Fiscal Year Final Research Report
Development of an anti-aging protocol and maintaining undifferentiated state of oral mucosa keratinocyte progenitor/stem cells by pharmacological manipulation of autophagy
| Project/Area Number |
22390371
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
IZUMI Kenji 新潟大学, 医歯学系, 准教授 (80242436)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
TERADA Michiko 新潟大学, 医歯学系, 特任助教 (60374550)
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| Project Period (FY) |
2010 – 2012
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| Keywords | オートファジー / mTOR / ラパマイシン / 口腔粘膜上皮 / 前駆/幹細胞 / ウェスタンブロッティング |
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| Research Abstract |
The TOR-autophagy spatial coupling compartment (TASCC) represents for the intracytoplasmic region where protein degradation and synthesis occur in unison to handle rapid protein turnover by integration of rough endoplasmic reticulum, Golgi apparatus, (auto)lysosomes and mTOR, which is beneficial to cellsto efficiently produce secretory proteins. In this study, we found the TASCC in primary cultured human oral keratinocytes. In addition, our results suggested Maspin and IL-6 produced by oral keratinocyteswere synthesized and secreted through TASCC.
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