2013 Fiscal Year Final Research Report
Study on the stemness of human dental pulp cells and the inductive efficiency for the safty iPS cells
| Project/Area Number |
22390383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Gifu University |
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Principal Investigator |
SHIBATA Toshiyuki 岐阜大学, 医学(系)研究科(研究院), 教授 (50226172)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEDA Tomoko (KAWAGUCHI Tomoko) 岐阜大学, 医学部附属病院, 医員 (30509815)
KATO Keizou 岐阜大学, 医学部附属病院, 講師 (40397336)
MAKITA Hiroki 岐阜大学, 大学院・医学系研究科, 講師 (50345790)
HATAKEYAMA Daijirou 岐阜大学, 医学部附属病院, 講師 (60377653)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 再生医学 / 歯髄 / iPS細胞 |
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| Research Abstract |
In this study, we examined the effects of hypoxia and cell stemness on the inductive efficiency for the safty iPS cell using human dental pulp cells. Our results were as follows; 1. Hypoxia condition(3%O2) improved the cell growth and establishment efficiency of primary culture. Furthermore, hypoxia condition was effective and useful for the maintenance of stro-1positive cells and high differential potency cells and we found out that the hypoxia condition could improve the efficiency of iPS cell induction and its quality. 2. We established the iPS cells efficiently using episomal plasmid as a gene-transferring vector without the foreign gene insertion. 3. We found out that the DLX4 might play an important role in the cell initiation and that the enhancement of the DLX4 expression could induce the highly efficient effect for the iPS cell establishment.
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[Journal Article] Periostin directly and indirectly promotes tumor lymphangiogenesis of head and neck cancer2012
Author(s)
Kudo Y, Iizuka S, Yoshida M, Nguyen PT, Siriwardena SB, Tsunematsu T, Ohbayashi M, Ando T, Hatakeyama D, Shibata T, Koizumi K, Maeda M, Ishimaru N, Ogawa I, Takata T
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Journal Title
PLoS One
Volume: 7(8)
Pages: e44488
DOI
Peer Reviewed
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[Journal Article] A more efficient method to generate integration-free human iPS cells2011
Author(s)
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S
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Journal Title
Nat Methods
Volume: 8(5)
Pages: 409-12
Peer Reviewed
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[Journal Article] Dental pulp cells for induced pluripotent stem cell banking2010
Author(s)
Tamaoki N, Takahashi K, Tanaka T, Ichisaka T, Aoki H, Takeda-Kawaguchi T, Iida K, Kunisada T, Shibata T, Yamanaka S, Tezuka K
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Journal Title
J Dent Res
Volume: 89(8)
Pages: 773-8
Peer Reviewed
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