2012 Fiscal Year Final Research Report
Jaw bone regeneration using gene activated matrix incorporated withdirectly prepared adipose derived stem cells
| Project/Area Number |
22390387
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
ASAHINA Izumi 長崎大学, 医歯薬学総合研究科, 教授 (30221039)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUMITA Yoshinori 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (50456654)
KOMORI Tosihisa 長崎大学, 大学院・医歯薬学総合研究科, 教授 (00252677)
NISHIMURA Masahiro 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00294570)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 口腔顎顔面再健外科学 |
|---|
| Research Abstract |
Adipose derived stem cells (ADSCs) have been isolated from adipose tissue by the simple method with collagenase digestion and centrifugation. Direct implantation of freshly isolated ADSCs with minimal amount of BMP could induce ectopic bone formation. ADSCs transfected with the plasmid carrying BMP and/or Wnt genes differentiated into osteoblasts in vitro. Implantation of the gene activated matrix (GAM) consisted with atelocollagen, which constructed with BMP gene by calcium precipitation method, induced bone formation and the addition of ADSCs to the GAM accelerated the bone induction.
|
-
-
-
-
[Journal Article] Chronological histological changes during bone regeneration on a non-crosslinked atelocollagen matrix2012
Author(s)
Kagawa R, Kishino M, Sato S, Ishida K, Ogawa Y, Ikebe K, Oya K, Ishimoto T, Nakano T, Maeda Y, Komori T, Toyosawa S
-
Journal Title
J Bone Miner Metab.
Volume: 30(6)
Pages: 638-50
-
-
-
-
-
