Analysis of the β catenin gene abnormality and therapeutic application for oral cancer.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390388
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Sapporo Medical University
• Principal Investigator: HIRATSUKA Hiroyoshi 札幌医科大学, 医学部, 教授 (50165180)
• Co-Investigator(Kenkyū-buntansha): DEHARI Hironari 札幌医科大学, 医学部, 助教 (00381260) ; MIYAZAKI Akihiro 札幌医科大学, 医学部, 准教授 (10305237) ; OGI Kazuhiro 札幌医科大学, 医学部, 訪問研究員 (40433114) ; SASAKI Takanori 札幌医科大学, 医学部, 研究員 (50548732) ; NAKAMORI Kenji 札幌医科大学, 医学部, 講師 (60295334) ; KOBAYASHI Jyunichi 札幌医科大学, 医学部, 助教 (80404739) ; SOGABE Youhei 札幌医科大学, 医学部, 研究員 (50468104) ; YAMAMOTO Takashi 札幌医科大学, 医学部, 研究員 (50448594) ; SHIMANISHI Makoto 札幌医科大学, 医学部, 研究員 (10516561)
• Project Period (FY): 2010 – 2012
• Keywords: 口腔がん / βカテニン / 低酸素 / 上皮-間葉転換 / GLUT-1 / NF-κB/p65

Research Abstract

Hypoxic regions in solid tumors mediate malignant transformation, cancer cell proliferation, resistance to anti-cancer agents, and enhanced cellular invasion and metastasis. In this study, we found that the wnt/β-catenin pathway activation mediates the acquisition of invasion and metastatic potential in oral squamous cell carcinoma cells. Hypoxia enhances the expression of glucose transporter 1 (GLUT1) and anticancer drug resistance, and activates NF-κB/p65 to trigger cell proliferation.

Research Products

• [Journal Article] Suppression of NF-κB/p65 inhibits the proliferation in oral squamous cancer cells. (2013)
  • Author(s): Naoki Anbo, Kazuhiro Ogi, Yohei Sogabe, Makoto Shimanishi, Takeshi Kaneko, Hironari Dehari, Akihiro Miyazaki, Hiroyoshi Hiratsuka.
  • Journal Title: Journal of Cancer Therapy Pages: 891-897
  • DOI: DOI:10.4236/jct.2013.44100
  • Peer Reviewed
• [Journal Article] Silencing of GLUT-1 inhibits sensitization of oral cancer cells to cisplatin during hypoxia. (2013)
  • Author(s): Shimanishi M, Ogi K, Sogabe Y, Kaneko T, Dehari H, Miyazaki A, Hiratsuka H.
  • Journal Title: J Oral Pathol Med. Volume: 42(5) Pages: 382-8
  • DOI: DOI:10.1111/jop.12028.
  • Peer Reviewed
• [Journal Article] Highexpression of ALDH1 and SOX2 diffuse staining pattern of oral squamous cellcarcinomas correlates to lymph node metastasis. (2013)
  • Author(s): Michifuri Y, Hirohashi Y, Torigoe T,Miyazaki A, Kobayashi J, Sasaki T, FujinoJ, Asanuma H, Tamura Y, Nakamori K, Hasegawa T, Hiratsuka H, Sato N.
  • Journal Title: Pathol Int. Volume: 62(10) Pages: 684-9
  • DOI: DOI:10.1111/j.1440-1827.2012.02851.x.
  • Peer Reviewed
• [Journal Article] Granulocyte colony-stimulating factor-producing squamous cellcarcinoma of the lower gingiva: a case report. (2012)
  • Author(s): Kobayashi J, Miyazaki A, Yamamot T, Nakamori K, Suzuki R, Kaneko T, Suzuki N, Hiratsuka H.
  • Journal Title: Head Neck Oncol. Volume: 4 Pages: 35
  • DOI: DOI:10.1186/1758-3284-4-35.
  • Peer Reviewed
• [Journal Article] Working Group 1 on the 'Guidelines for Clinical and Pathological Studies of Oral Cancer', Scientific Committee, Japan Society for Oral Tumors.General rules for clinical and pathological studies on oral cancer: a synopsis. (2012)
  • Author(s): Izumo T, Kirita T, Ariji E, Ozeki S, Okada N, Okabe S, Okazaki Y, Omura K, Kusama M, Sato T, Shinohara M, Shimozato K, Shintani S, Tanaka Y, Nakayama E, Hayashi T, Miyazaki A, Yagishita H, Yamane M
  • Journal Title: Jpn J Clin Oncol. Volume: 42 Pages: 1099-109
  • Peer Reviewed
• [Journal Article] 口腔悪性腫瘍. (2012)
  • Author(s): 宮崎晃亘, 平塚博義.
  • Journal Title: 医学と薬学 Volume: 67 Pages: 799-805
• [Journal Article] Phase I clinical trial of survivin-derived peptide vaccine therapy for patients with advanced or recurrent oral cancer. (2011)
  • Author(s): Miyazaki A, Kobayashi J, Torigoe T, Hirohashi Y, Yamamoto T, Yamaguchi A,Asanuma H, Takahashi A, Michifuri Y, Nakamori K, Nagai I, Sato N, Hiratsuka H.
  • Journal Title: Cancer Sci. Volume: 102(2) Pages: 324-9
  • DOI: DOI:10.1111/j.1349-7006.2010.01789.x.
  • Peer Reviewed
• [Presentation] The stressed cells are potential targets for CD8+T cell recognition in vivo (2012)
  • Author(s): Takanori Sasaki, Takayuki Kanaseki, Sachiyo Kawami, Yasuaki Tamura, Toshihiko Torigoe, Hiroyoshi Hirastuka, Noriyuki Sato.
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 20121200
• [Presentation] Silencing of GLUT1 inhibits sensitizes oral cancer cells to CDDP during hypoxia (2012)
  • Author(s): Kazuhiro Ogi, Makoto Shimanishi, Takeshi Kaneko, Hironari Dehari, Akihiro Miyazaki, Hiroyoshi Hiratsuka.
  • Organizer: 日本癌学会総会
  • Place of Presentation: 札幌
  • Year and Date: 20120900
• [Presentation] Survivin-derived peptide vaccine therapy for patients with advanced or recurrent oral cancer - Phase I clinical trial (2012)
  • Author(s): Akihiro Miyazaki, Junichi Kobayashi, Toshihiko Torigoe, Yoshihiko Hirohashi, Yoshitaka Michifuri, Takanori Sasaki, Junki Fujino, Hiroko Asanuma, Akari Takahashi, Noriyuki Sato, Hiroyoshi Hiratsuka.
  • Organizer: 日本癌学会総会
  • Place of Presentation: 札幌
  • Year and Date: 20120900
• [Presentation] Analysis of cDNA plate array between CDDP sensitive and CDDP resistant. Oral squamous cancer cell line ; HIF-1 can regulate NF-κB under hypoxia (2011)
  • Author(s): Naoki Anbo, Makoto Shimanishi, Kazuhiro Ogi, Hiroyoshi Hiratsuka
  • Organizer: 日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] Identification of epigenetic change of genes that mediates hypoxic adaptation using HIF regulated cDNA plate array. (2011)
  • Author(s): Kazuhiro Ogi, Makoto Shimanishi, Naoki Anbo, Tsuyoshi Kaneko, Yohei sogabe, Hironari Dahari, Hiroyoshi Hiratsuka
  • Organizer: 日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] A phase I study of survivin-2B peptide vaccine therapy in combination with interferon-α for patients with oral cancer. (2011)
  • Author(s): Akihiro Miyazaki, Junichi Kobayashi, Toshihiko Torigoe, Yoshihiko Hirohashi, Yoshitaka Michifuri, Takanori Sasaki, Hiroko Asanuma, Noriyuki Sato, Hiroyoshi Hiratsuka
  • Organizer: 日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] The cells under the ER stress present novel pMHCI and elict CD8+T cell responses. (2011)
  • Author(s): Takanori Sasaki, Takayuki Kanaseki, Hiroyoshi Hiratsuka, Noriyuki Sato
  • Organizer: 日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] Small proline-rich protein-1B (SPRP1B) is overexpressed in ALDEFLUOR bright human cancer stem-like oral squamous cell carcinoma and related to its maintenance. (2011)
  • Author(s): Yoshitaka Michifuri, Yoshihiko Hirohashi, Toshihiko Torigoe, Akihiro Miyazaki, Junichi Kobayashi, Takashi Yamamoto, Yasuaki Tamura, Akari Takahashi, Kenji Nakamori, Akira Yamaguchi, Hiroyoshi Hiratsuka, Noriyuki Sato
  • Organizer: 日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] The cells under the ER stress present novel pMHCI and elicit CD8+ T cell responses (2011)
  • Author(s): Takanori Sasaki, Takayuki Kaneseki, Emiri Nakazawa, Eri Saka, Yasuaki Tamura, Toshihiko Torigoe, Hiroyoshi Hiratsuka,Noriyuki Sato
  • Organizer: The 2011 CSSI Congress
  • Place of Presentation: カナダ、ケベック
  • Year and Date: 20110800
• [Presentation] 小胞体ストレスによりMHCクラスI抗原プロセシングの分子機構と病態 (2011)
  • Author(s): 佐々木敬則、金関貴幸、山本絵利、植田恵実理、田村保明、鳥越俊彦、平塚博義、佐藤昇志
  • Organizer: 日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20110400
• [Presentation] 口腔がんにおけるがん幹細胞特異的分子の同定 (2011)
  • Author(s): 道振義貴、廣橋良彦、鳥越俊彦、宮崎晃亘、小林淳一、田村保明、平塚博義、佐藤昇志
  • Organizer: 日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20110400
• [Presentation] 口腔癌に対するサバイビン2Bペプチドワクチン療法の第I臨床試験 (2010)
  • Author(s): 宮崎晃亘、小林淳一、鳥越俊彦、廣橋良彦、山本 崇、道振義貴、浅沼広子、荻 和弘、山口 晃、佐藤昇志、平塚博義
  • Organizer: 日本癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20100900
• [Presentation] 口腔癌における癌低酸素領域での血管新生因子に関する臨床的意義 (2010)
  • Author(s): 荻 和弘、仲盛健治、宮崎晃亘、島西真琴、安保直樹、平塚博義
  • Organizer: 日本癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20100900
• [Presentation] hMHL1, hMSH2の口腔扁平上皮癌の抗癌剤耐性獲得への関与に関する研究 (2010)
  • Author(s): 安保直樹、島西真琴、荻 和弘、平塚博義
  • Organizer: 日本癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20100900
• [Presentation] Clinical Significance of Cortactin and Cyclin D1 Expression in Squamous Cell Carcinomas of the Tongue (2010)
  • Author(s): Kenji Nakamori, Hironari Dehari, Takashi Imai, Akihiro Miyazaki, Kazuhiro Ogi, Naoki Anbo, Makoto Shimanishi, Hiroyoshi Hiratsuka
  • Organizer: 20th European Association for Cranio-Maxillo-facial Surgery
  • Place of Presentation: ベルギー
  • Year and Date: 20100900
• [Book] Anatomy, Kinematics and Diseases (2012)
  • Author(s): Nakamori K, Dehari H, Miyazaki A, Ogi K, Anbo N, Shimanishi M, Kaneko T, Hiratsuka H
  • Total Pages: 155-164
  • Publisher: Nova Science Publishers
• [Remarks]
  • URL: http://web.sapmed.ac.jp/oral/