Elucidation of autoummune condition in periodontitis-accelerated atherosclerosis and control by oral tolerance

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390398
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Nihon University
• Principal Investigator: OCHIAI Tomoko 日本大学, 松戸歯学部, 准教授 (20130594)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Masafumi 日本大学, 松戸歯学部, 教授 (80210558) ; OGUCHI Sumito 日本大学, 松戸歯学部, 准教授 (30366611) ; OGATA Yorimasa 日本大学, 松戸歯学部, 教授 (90204065) ; HASHIZUME Tomomi 日本大学, 松戸歯学部, 助手 (50419785)
• Project Period (FY): 2010 – 2012
• Keywords: 感染 / 歯周病 / 歯周病原細菌 / 動脈硬化

Research Abstract

Not only live bacteria but also the cellular components, such as LPS have been participated in acceleration of the atherosclerosis by periodontopathic bacteria,and affected expression of TLR,MLR and oxidant stress,or HSP. The increase in autoantigens by periodontal infection was TLR dependent and correlated with the increase in autoantibody in clinical samples.Furthermore, it was suggested that oxidative modefication of LDL by periodontopathic bacteria induced autoimmune disorder.In contrast, immunization with P.gingivalis GroEL suppressed atherosclerosis with the reduction of inflammatory markers and autoimmune markers. These results suggest that oral tolerance induction is effective in reducing arteriosclerosis.

Research Products

• [Journal Article] Periodontal pathogen accelerates lipid peroxidation and atherosclerosis (2013)
  • Author(s): Jia,R.,Kurita-Ochiai,T.,Oguchi,S.,Yamamoto,M.
  • Journal Title: J. Dent.Res. Volume: 93(3) Pages: 247-252
  • Peer Reviewed
• [Journal Article] Green tea epigallocathecin-3-gallate attenuates Porphyromonas gingivalis-induced atherosclerosis. (2013)
  • Author(s): Cai,Y., Kurita-Ochiai, T.,Hashizume-Takizawa,T.,Yamamoto,M.
  • Journal Title: Pathogens and Disease. Volume: 67 Pages: 76-83
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis accelerates atherosclerosis in C57BL/6 mice fed a high -fat diet. (2012)
  • Author(s): Fukasawa,A,.kurita-Ochiai,T.,Hashizume,T.,Kobayashi,R.,Akimoto,Y.,Yamamoto,M.
  • Journal Title: Immunopharmacol.Immunotoxicol Volume: 34(3) Pages: 481-487
  • Peer Reviewed
• [Journal Article] Sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin A of Porphyromonas gingivalis and Escherichacoli malyose-binding protein elicits protective immunity in the oral cavity. (2012)
  • Author(s): Yuzawa,S.,kurita-Ochiai,T.,Hashizume,T.,Kobayashi,R.,Abiko,Y.,Yamamoto,M.
  • Journal Title: FEMS Immunol.Med.Microbio. Volume: 64 Pages: 265-272
  • Peer Reviewed
• [Journal Article] Intranasal immunization with Porphyromonas gingivalis outer membrane protein inhibits P.gingivalis-induced atherosis in C57BL/6 mice fed a high-fat diet. (2012)
  • Author(s): Fukasawa,A,.kurita-Ochiai,T.,Hashizume,T.,Kobayashi,R.,Akimoto,Y.,Yamamoto,M.
  • Journal Title: Int.J.Oral-Med.Sci. Volume: 10(4) Pages: 362-371
  • Peer Reviewed
• [Journal Article] Nasal immunization with a fusion protein consisting of the hemagglutinin a antigenic region and the maltose-binding protein elicits CD11C+ CD8+ dendriticcells for induced long-term protective immunity. (2011)
  • Author(s): Du,Y.,Hashizume,T.,kurita-Ochiai,T.,Yuzawa,S.,Abiko,Y.,and Yamamoto,M.
  • Journal Title: Infect. Immun. Volume: 79(2) Pages: 895-904
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis stimulates monocyte adhesion to human umbilical vein endothelial cells. (2011)
  • Author(s): Hashizume,T.,kurita-Ochiai,T.,Yamamoto,M.
  • Journal Title: FEMS Immunol.Med.Microbio. Volume: 62(1) Pages: 57-65
  • Peer Reviewed
• [Journal Article] A fusion protein of the hemagglutinin Aantigenic region of Porphyromonas gingivalis and maltose-binding proteinduces antibody responses in the oralcavity (2011)
  • Author(s): Du,Y.,Hashizume,T.,kurita-Ochiai,T.,Yuzawa,S.,Abiko,Y.,and Yamamoto,M
  • Journal Title: Int.J.Oral-Med.Sci. Volume: 9(3) Pages: 182-189
  • Peer Reviewed
• [Journal Article] Aggregatibacter Actinomycetemcomitans accelerates atherosclerosis with an increase in atherogenic factors in spontaneously hyperlipidemic mice. (2010)
  • Author(s): Zhang,T.,kurita-Ochiai,T.,Hashizume.,T.,Du,Y.Oguchi, S.,Yamamoto,M
  • Journal Title: FEMS Microbiol.Immunol Volume: 59 Pages: 143-151
  • Peer Reviewed
• [Journal Article] Oral immunization with outer membrane protein of Porphyromonas gingivalis and CpGoligodeoxynucleotides elicits both Thelper 1 and 2 cytokines for enhanced protective immunity. (2010)
  • Author(s): Liu, C. L.,Hashizume.,T kurita-Ochiai,T.Fujihashi,K.,Yamamoto,M.
  • Journal Title: Mol.Oral.Microbiol. Volume: 25(3) Pages: 178-189
  • Peer Reviewed
• [Journal Article] Aggregatibacter Actinomycetemcomitans leads to endothelial apoptosis and atherosclerosis development in spontaneously hyperlipidemic mice. (2010)
  • Author(s): Zhang,T.,kurita-Ochiai,T.,Hashizume.,T.,Oguchi, S.,Abiko,Y.,Yamamoto,M.
  • Journal Title: Int.J.Oral-Med.Sci. Volume: 8(3) Pages: 132-141
  • Peer Reviewed
• [Journal Article] Mucosal Immunity in CD4-Deficientmicd:Direct evidence for mucosal IgAantibody responses. (2010)
  • Author(s): Hashizume.,T.,Tanaka,S.,Kaminogawa,S.,Hosono, A., Kataoka, K.,Shinozaki-Kuwahara,N.,Kweon,M.N.,kurita-Ochiai,T.,Yamamoto,M.
  • Journal Title: Int.J.Oral-Med.Sci Volume: 8(3) Pages: 151-156
  • Peer Reviewed
• [Journal Article] Effect of Porphyromonas gingivalis on human umbilical veinendo thelial cells. (2010)
  • Author(s): Hashizume.,T.,kurita-Ochiai,T.,Mikuni,D.,kawanabe,K.,Kanamaru,S.,Yamamoto,M.
  • Journal Title: Int.J.Oral-Med.Sci. Volume: 8(3) Pages: 157-161
  • Peer Reviewed
• [Journal Article] Butyricacid induces apoptosis via oxidative stress in Jurkat T-cells. (2010)
  • Author(s): kurita-Ochiai,T.,Ochiai K.
  • Journal Title: J.Dent.Res. Volume: 89(7) Pages: 689-694
  • Peer Reviewed
• [Journal Article] antibodies against Porphyromonas gingivalis GroEL are insufficient to induce P.gingivalis-accelerated atherosclerosis.
  • Author(s): Hagiwara,M., Kurita-Ochiai, T.Hashizume-Takizawa, T.,Yamamoto,M.Serum
  • Journal Title: Int.J.Oral-Med.Sci Volume: (in press)
  • Peer Reviewed
• [Journal Article] Nasal administration of glucosyltransferase-I of streptococcus sobrinus without adjuvant induces protective immunity.
  • Author(s): Watanabe k.,Hashizume.,T.,kurita-Ochiai,T.,Akimoto Y.and Yamamoto M.
  • Journal Title: Journal of Vaccines & Vaccination
  • DOI: doi:10.4172/2157-7560.1000109,2010
  • Peer Reviewed
• [Presentation] Nasal vaccine against outer membrane protein of periodontal bacteria-induced atherosclerosis (2012)
  • Author(s): Oguchi,S.,Sakamaki,T.,Kurita-Ochiai,T.,Inoue,F.,Nagao,K.
  • Organizer: 日本循環器学会(シンポジウム)
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-03-16
• [Presentation] Porphyromonas gingivalis による低比重リポタンパクの酸化変性. (2011)
  • Author(s): kurita-Ochiai,T.Hashizume.T.,Yamamoto M.
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 幕張メッセ(秋葉)
  • Year and Date: 2011-11-29
• [Book] 口腔微生物学ー感染と免疫 (2013)
  • Author(s): 落合智子他
  • Total Pages: 224-278
  • Publisher: 学健書院東京