Structure and detection of toxins from dinoflagellate

2014 Fiscal Year Final Research Report

• Project/Area Number: 22404006
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: SATAKE Masayuki 東京大学, 理学(系)研究科(研究院), 准教授 (90261495)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 構造決定 / 定量法開発

Outline of Final Research Achievements

A red tide occurred in New Zealand. This caused mass mortalities of fish within the harbour. Over 500 cases of human respiratory distress were also reported, with symptoms including a dry cough, severe sore throat, and headache. A cell extract of the causataive dinoflagellate showed strong mouse lethality and cytotoxicity.
From the neutral lipophilic fraction five novel toxins were identified. Isolation was guided by cytotoxicity against mouse leukemia P388 cells. The sodiated cations of toxins in MALDI mass spectra were observed at over m/z 2000. The most abundant toxin was determined to be a large polycyclic ether compound with 24 ether rings, including dihydrofuran, 13 hydroxy groups, and a 2-methylbut-2-enal side chain. From the hydrophilic acidic fraction another novel set of toxins were identified. The toxins consist of nine contiguous ether rings and contain a 9-membered ether ring within the central portion of the molecule. Toxins showed cytotoxicity against neuro2A cells.

Free Research Field

天然物化学