Analyses of reproductive isolation of Closterium peracerosum-strigosum-littorale complex for the understanding of the speciation.

2013 Fiscal Year Final Research Report

• Project/Area Number: 22405014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Women's University
• Principal Investigator: SEKIMOTO Hiroyuki 日本女子大学, 理学部, 教授 (20281652)
• Co-Investigator(Kenkyū-buntansha): NOZAKI Hisayoshi 東京大学, 大学院理学系研究科, 准教授 (40250104)
• Co-Investigator(Renkei-kenkyūsha): TSUCHIKANE Yuki 日本女子大学, 理学部, 助教 (20434152) ; ABE Jun 日本女子大学, 理学部, 学術研究員 (10424764)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Keywords: ミカヅキモ / 生殖隔離 / 性フェロモン / 種分化

Research Abstract

The Closterium peracerosum-strigosum-littorale complex (C. p-s-l. complex) is a unicellular alga, and is known to be composed of several reproductively isolated mating groups of heterothallic strains. We carried out field studies in foreign countries and in Japan, to isolate reproductively isolated C. p-s-l. complex. Finally, we established several unialgal clonal cultures from these fields.
We studied phylogenetic relationship using DNA sequences of 1506 group I introns of these clones. Based on the alignment, significant phylogenetic relationships were observed between newly established mating group G and mating group I-E. Because genome project of cells belonging to the group I-E is ongoing, newly established group G would be suitable for the understanding of the mechanism of reproductive isolation, in the molecular biological aspect.

Research Products

• [Journal Article] Characterization of sexual reproductive processes in Chara braunii (Charales, Charophyceae) (2014)
  • Author(s): Sato, M., Sakayama, H., Sato, M., Ito, M., Sekimoto, H
  • Journal Title: Phycol. Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] The evolution of Male-Female sexual dimorphism predates the gender-based divergence of mating locus gene Mat3/RB (2013)
  • Author(s): Hiraide, R., Kawai-Toyooka, H., Hamaji, T., Matsuzaki, R., Kawafune, K., Abe, J., Sekimoto, H., Umen, J., Nozaki, H
  • Journal Title: Mol. Biol. Evol Volume: 30(5) Pages: 1038-1040
  • DOI: 10.1093/molbev/mst018
  • Peer Reviewed
• [Journal Article] New insights into the regulation of sexual reproduction in Closterium (2012)
  • Author(s): Sekimoto, H., Abe, J., Tsuchikane, Y
  • Journal Title: International Review of Cell & Molecular Biology Volume: 297 Pages: 309-338
  • DOI: 10.1016/B978-0-12-394308-8.00014-5
• [Journal Article] Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum-strigosum-littorale complex (Zygnematophyceae, Charophyta) (2012)
  • Author(s): Hori, S., Sekimoto, H., Abe, J
  • Journal Title: Phycol. Res Volume: 60 Pages: 254-260
  • DOI: 10.1111/j.1440-1835.2012.00656.x
  • Peer Reviewed
• [Journal Article] ミカヅキモの生殖について (2012)
  • Author(s): 土金勇樹、関本弘之
  • Journal Title: 海洋と生物 Volume: 34巻、5号 Pages: 426-434
• [Journal Article] Zygospore formation between homothallic and heterothallic strains of Closterium (2012)
  • Author(s): Tsuchikane, Y., Tsuchiya, M., Hindak, F., Nozaki, H., Sekimoto, H
  • Journal Title: Sex Plant Reprod Volume: 25 Pages: 1-9
  • DOI: 10.1007/s00497-011-0174-z
  • Peer Reviewed
• [Journal Article] 単細胞シャジクモ藻類ミカヅキモにおける生殖隔離、種分化機構の研究 (2012)
  • Author(s): 関本弘之
  • Journal Title: IFO Research communications Volume: 26巻 Pages: 71-85
  • Peer Reviewed
• [Journal Article] An extended phylogenetic analysis reveals ancient origin of"non-green"phosphoribulokinase genes from two lineages of"green"secondary photosynthetic eukaryotes : Euglenophyta and Chlorarachniophyta (2011)
  • Author(s): Yang, Y., Maruyama, S., Sekimoto, H., Sakayama, H., Nozaki, H
  • Journal Title: BMC Research Note
  • DOI: 10.1186/1756-0500-4-330
  • Peer Reviewed
• [Journal Article] Stable nuclear transformation of Closterium peracerosum-strigosum-littorale complex (2011)
  • Author(s): Abe, J., Hori, S., Tsuchikane, Y., Kitao, N., Kato, M., Sekimoto, H
  • Journal Title: Plant Cell Physiol Volume: 52(9) Pages: 1676-1685
  • DOI: 10.1093/pcp/pcr103
  • Peer Reviewed
• [Journal Article] Conjugation processes of Penium margaritaceum (Zygnematophyceae, Zygnematophyta) (2011)
  • Author(s): Tsuchikane, Y., Tsuchiya, M., Kokubun, Y., Abe, J., Sekimoto, H
  • Journal Title: Phycol. Res Volume: 59 Pages: 74-82
  • DOI: 10.1111/j.1440-1835.2010.00602.x
  • Peer Reviewed
• [Journal Article] Characterization and molecular cloning of conjugation- regulating sex pheromones in homothallic Closterium (2010)
  • Author(s): Tsuchikane, Y., Kokubun, Y., Sekimoto, H
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 1515-1523
  • DOI: 10.1093/pcp/pcq110
• [Journal Article] Intercellular localization of an endogenous cellulose synthase of Micrasterias denticulate (Desmidiales, Chlorophyta) by means of transient genetic transformation (2010)
  • Author(s): Vannerum, K., Abe, J., Sekimoto, H., Inze, D., Vyverman, W
  • Journal Title: J. Phycol Volume: 46 Pages: 839-845
  • DOI: 10.1111/j.1529-8817.2010.00867.x
  • Peer Reviewed
• [Journal Article] Sexual processes and phylogenetic relationships of a homothallic strain in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae) (2010)
  • Author(s): Tsuchikane, Y., Sato., M., Ootaki, T., Kokubun, Y., Nozaki, H., Ito, M., Sekimoto, H
  • Journal Title: J. Phycol Volume: 46 Pages: 278-284
  • DOI: 10.1111/j.1529-8817.2010.00806.x
  • Peer Reviewed
• [Presentation] Characterization of a conjugation processes in homothallic Closterium peracerosum-strigosum-littorale complex (2013)
  • Author(s): Tsuchikane, Y., Sekimoto, H
  • Organizer: 10th International phycological congress
  • Place of Presentation: Orlando, Florida USA
  • Year and Date: 2013-08-09
• [Presentation] A receptor-like kinase responsible for the progress of sexual reproduction of a charophycean alga, Closterium peracerosum-strigosum-littorale complex (2013)
  • Author(s): Sekimoto, H., Hirano, N., Marukawa, Y., Abe, J., Tsuchikane, Y
  • Organizer: 10th International phycological congress, Orlando
  • Place of Presentation: Florida USA
  • Year and Date: 2013-08-06
• [Presentation] 生殖隔離に関わる性フェロモンのゲノムレベルでの特徴 (2013)
  • Author(s): 土金勇樹、西山智明、関本弘之
  • Organizer: 日本藻類学会第37回大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2013-03-28
• [Presentation] Intercellular communication in sexual reproduction of a unicellular charaphycean alga, Closterium peracerosum-strigosum-littorale complex (2012)
  • Author(s): Sekimoto, H., Hirano, N., Marukawa, Y., Tsuchikane, Y., Abe, J
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Nagoya
  • Year and Date: 2012-11-16
• [Presentation] Sexual recognition between homothallic and heterothallic strains of Closterium (2012)
  • Author(s): Tsuchikane, Y., Sekimoto, H
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Nagoya
  • Year and Date: 2012-11-15
• [Presentation] ヒメミカヅキモの性フェロモンPR-IP Inducer結合分子の特性解析 (2012)
  • Author(s): 関本弘之、滝口若菜、原真由美、高橋紗也香、土金勇樹、阿部淳
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Presentation] 接合藻ヒメミカヅキモにおける生殖隔離に関わる性フェロモンの進化 (2012)
  • Author(s): 土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Presentation] Identification of binding molecules for sex pheromone, PR-IP Inducer, in Closterium peracerosum-strigosum-littorale complex (2011)
  • Author(s): Sekimoto, H
  • Organizer: The 6th Asian Pacific Phycological Forum
  • Place of Presentation: Yeosu, Korea
  • Year and Date: 2011-10-13
• [Presentation] 単細胞シャジクモ藻類ヒメミカヅキモのゲノミクス (2011)
  • Author(s): 阿部淳、西山智明、関本弘之
  • Organizer: 日本植物学会 シンポジウム「ケノム解析によって解き明かされる植物の多様性」
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-18
• [Presentation] 単細胞接合藻類ヒメミカヅキモの性フェロモンPR-IP Inducer結合分子の探索 (2011)
  • Author(s): 関本弘之、原真由美、高橋紗也香、山田紗也佳、宮城絢乃、土金勇樹、阿部淳
  • Organizer: 日本植物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-09-17
• [Presentation] 接合藻ヒメミカヅキモにおけるホモタリック株の接合は性フェロモン(PR-IP Inducer)により促進される (2010)
  • Author(s): 土金勇樹、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-09
• [Presentation] 単細胞接合藻ヒメミカヅキモのホモタリック株は、「性分化」する (2010)
  • Author(s): 土屋美紀、土金勇樹、関本弘之
  • Organizer: 日本植物学会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-09
• [Book] 第4章藻類の有性生殖(澤田均編著「動植物の受精学」 (2014)
  • Author(s): 野崎久義、関本弘之
  • Publisher: 化学同人(京都)
• [Book] Mechanism of sexual reproduction in fresh water microalgae. In"Reproductive Biology of Plants" (2014)
  • Author(s): Sekimoto, H., Abe, J., Tsuchikane, Y
  • Publisher: Ramawat, K.G. ed. CRC press
• [Book] Sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strogosum-littorale complex. In"Sexual Reproduction in Animals and Plants" (2014)
  • Author(s): Sekimoto, H., Tsuchikane, Y., Abe, J
  • Publisher: Sawada, H. et al. eds. Springer
• [Book] 性フェロモン(渡辺信他編著「藻類ガイドブック」 (2012)
  • Author(s): 関本弘之
  • Publisher: エヌ・ティー・エス