Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

2012 Fiscal Year Final Research Report

• Project/Area Number: 22406011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Oita University
• Principal Investigator: KAWAMOTO Fumihiko 大分大学, 全学研究推進機構, 教授 (40115556)
• Co-Investigator(Kenkyū-buntansha): KANBE Toshio 名古屋大学, 大学院・医学系研究科, 講師 (50093018)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Masatsugu 大阪市立大学, 大学院・医学研究科, 准教授 (60195378) ; OTSUKA Yasushi 大分大学, 医学部, 助教 (00244161)
• Project Period (FY): 2010 – 2012
• Keywords: 国際研究者交流 / インドネシア:ベトナム:カンボジア / 熱帯熱 マラリア原虫 / 東南アジア / 分布領域 / 分子疫学調査 / 遺伝子解析 / 人工 培養

Research Abstract

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.

Research Products

• [Journal Article] Preservation of wild isolates of human malaria parasites in wet ice and adaptation efficacy to in vitro culture (2012)
  • Author(s): I.S. Tantular, S. Pusarawati, L. Khin, T. Kanbe, M. Kimura, Y. Kido, F. Kawamoto
  • Journal Title: Trop. Med. Health Volume: 40 Pages: 37-45
  • DOI: DOI:10.2149/tmh.2012-07o
  • Peer Reviewed
• [Journal Article] Genotypes of Candida albicans involved in development of candidiasis and their distribution in oral cavity of non-candidiasis individuals (2011)
  • Author(s): Y. Takagi, H. Hattori, H. Adachi, S. Takakura, T. Horii, A. Chindamporn, H. Kitai, R. Tanaka, T. Yaguchi, H. Fukano, F. Kawamoto, K. Shimozato, T. Kanbe
  • Journal Title: Med. Mycol. J. Volume: 52 Pages: 315-324
  • URL: http://www.jstage.jst.go.jp/brawse/jjmm/-chan/ja/
  • Peer Reviewed
• [Journal Article] Micro- satellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis (2011)
  • Author(s): K. Shimizu, H. Hattori, H. Adachi, R. Ohshima, T. Horii, R. Tanaka, T. Yaguchi, M. Akiyama, F. Kawamoto, T. Kanbe
  • Journal Title: Med Mycol J Volume: 52 Pages: 129-138
  • URL: http://www.jstage.jst.go.jp/brawse/jjmm/-chan/ja/
  • Peer Reviewed
• [Journal Article] 溶血発作で発症したグルコース6リン酸脱水素酵素異常症の早産児の 1 例 (2011)
  • Author(s): 赤澤洋平、馬場 淳、伊藤有香子、斉田 謙、平林香奈枝、三澤 由佳、石田岳史、服部幸夫、藤井寿一、川本文彦、松岡裕之
  • Journal Title: 日本未熟児新生児学会雑誌 Volume: 23 Pages: 108-112
  • URL: http://plaza.umin.ac.jp/jspn/
  • Peer Reviewed
• [Journal Article] Single nucleotide poly- morphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax (2010)
  • Author(s): P. Orjuela-Sanchez, N.D.Karunaweera, M.Da Silva-Nunes, N.S. da Silva, K.K. Scopel, R.M. Goncalves, C. Amaratunga, J.M. Sa, D. Sochet, R.M. Fairhust, Thava-. kodirasah, G.L. Galapathy, F. Kawamoto, M.U. Ferreira
  • Journal Title: BMC Genetics Volume: 11:65
  • DOI: DOI:10.1186/1471-2156-11-65
  • Peer Reviewed
• [Journal Article] Incidence and mutation analysis of glucose-6-phosphate dehydrogenase deficiencyin eastern Indonesian populations (2010)
  • Author(s): I.S. Tantular, H. Matsuoka, Y. Kasa hara, S Pusarawati, T. Kanbe, J.S. Tuda,Y. Kido, Y.P. Dachlan, F. Kawamoto
  • Journal Title: Acta Medica Okayama Volume: 64 Pages: 367-373
  • URL: http://escholarship.lib.okayama-u.ac.jp/amo/
  • Peer Reviewed
• [Presentation] Molecular epidemiology on malaria and G6PD deficiency in SEA (2012)
  • Author(s): I.S. Tantular & F. Kawamoto
  • Organizer: ICOPA XII
  • Place of Presentation: Rio de Janeiro, Brazil
  • Year and Date: 20120900
• [Presentation] 「東南アジアにおけるマラリア の分子疫学的研究」に至った長い道のり (2012)
  • Author(s): 川本文彦
  • Organizer: 日本熱帯医学会・学会賞受賞講演
  • Place of Presentation: 仙台
  • Year and Date: 2012-12-03
• [Presentation] Molecular epidemiology of malaria and G6PD deficiency in SEA (2011)
  • Author(s): F. Kawamoto
  • Organizer: International Symposium on emerging and re-emerging infectious diseases
  • Place of Presentation: Manado, Indonesia
  • Year and Date: 20111100
• [Presentation] Population structure of Plasmodium vivax (2010)
  • Author(s): F. Kawamoto
  • Organizer: The second Int. Meeting on Plasmodium vivax
  • Place of Presentation: Shanghai, China
  • Year and Date: 20100400
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/rpp/