2012 Fiscal Year Final Research Report
Study on cortical circuit using viral vectors:Species/area-comparison of layer 6 neurons
| Project/Area Number |
22500300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
WATAKABE Akiya 基礎生物学研究所, 脳生物学研究部門, 准教授 (40290910)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 大脳皮質回路 / ウイルスベクター / 蛍光タンパク質 / レンチウイルス / 逆行性感染 / TET誘導 / 6層ニューロン / マーモセット |
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| Research Abstract |
The specific aim of this study was to classify and characterize the excitatory pyramidal neurons that constitute the cortical circuit. I obtained the following two new findings. (1) By combining the retrograde tracers with the in situ hybridization method, I found that CCK and PCP4 genes are expressed in the cortical and thalamic projection neurons in layer 6. Furthermore, the area differences of their distribution were revealed. (2) I developed a novel lentiviral-based retrograde vector system that carries TET-Off components for high-level transgene expression. This vector was used to visualize the callossal neurons of the marmoset (New World monkey) cortex.
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