2012 Fiscal Year Final Research Report
The role of Collapsin Response Mediator Protein 4(CRMMP4) in sexual differentiation of the brain
| Project/Area Number |
22500315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Toyo University |
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Principal Investigator |
KANEKO Ritsuko (OHTANI Ritsuko) 東洋大学, 生命科学部, 教授 (00161183)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Naoya 横浜市立大学, 医学部, 助教 (40508793)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 脳の性分化 / 前腹側脳室周囲核 / CRMP4 / プロテオミクス解析 / ノックアウトマウス / ドーパミン作動性ニューロン |
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| Research Abstract |
Proteomics analysis of protein expression in the sexually dimorphic AVPV on postnatal day 1 (PD1; the early phase of sex differentiation) identified collapsin response mediator protein 4 (CRMP4) as a protein exhibiting sexually dimorphic expression. This sexually differential expression of CRMP4 protein and mRNA in the AVPV was not detected on PD6. Next, we used CRMP4-knockout (CRMP4-KO) mice to determine the in vivo function of CRMP4 in the AVPV. The number of tyrosine hydroxylase (TH)-ir neurons increased in the AVPV of adult female CRMP4-KO mice as compared with the adult female wild-type mice. No significant difference in the number of TH-ir neurons was detected between sexes or genotypes on embryonic day 15, but a female-specific increase in TH-ir neurons was observed in CRMP4-KO mice on PD1. These results indicate that CRMP4 regulates the number of TH-ir cell number in the female AVPV.
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[Journal Article] Collapsin response mediator protein 4 affects the number of tyrosine hydroxylase-immunoreactive neurons in the sexually dimorphic nucleus in female mice.2013
Author(s)
Iwakura T, Sakoh M, Tsutiya A, Yamashita N, Ohtani A, Tsuda M, Ogawa S, Tsukahara S, Nishihara M, Shiga T, Goshima Y, Kato T, Ohtani-Kaneko R.
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