Molecular mechanisms underlying axonal local protein synthesis in neurons

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500329
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Niigata University
• Principal Investigator: TAKEI Nobuyuki 新潟大学, 脳研究所, 准教授 (70221372)
• Co-Investigator(Renkei-kenkyūsha): NAMBA Hisaaki 新潟大学, 脳研究所, 助教 (90332650)
• Project Period (FY): 2010 – 2012
• Keywords: 細胞内情報伝達

Research Abstract

Translation machinery in growth cone. Existence of ribosomes in growth cone was investigated by immunocytochemistry with anti-ribosomalprotein antibodies. Although it is widely believed that there is no ribosomes in axon, immunoreactivityagainst ribosomal proteins was recognized in growth cone of cultured dorsal root ganglia neurons and ofhippocampal neurons. In addition, several translation factors were found in growth cone of theseneurons. Precise morphological analysis of growth cone was performed by using atomic forcemicroscopy.
Triggers of local translation in growth cone.Brain-derived neurotrophic factor (BDNF) and Wnt3a were identified as a trigger of local translation ingrowth cone. In addition to the [35S]methionin incorporation, puromycin-anti-puromycin immunocytochemistry method revealed that these factors activate novel protein synthesis in growth cones.
These results indicate the existence of translational machinery in growth cone and strongly suggest thatlocal protein synthesis occurs in growth cones in response to certain growth factors.

Research Products

• [Journal Article] Interactions between subcorticalheterotopia and overlying hippocampus mediated by NMDA receptors in humanbrain slices (2012)
  • Author(s): Kitaura H, Oishi M, Takei N, Fu YJ, Hiraishi T, Fukuda M, Takahashi H,Shibuki K, Fujii Y, and Kakita A
  • Journal Title: Epilepsia Jul Volume: 53(7) Pages: e127-131
• [Journal Article] Cell-surface expression of themajor Aβdegrading enzyme, neprilysin,depends on phosphorylation by MEK anddephosphorylation by protein phosphatase1a (2012)
  • Author(s): Kakiya N, Saito T, Nilsson P, Matsuba Y,Tsubuki S, Takei N, Nawa H, Saido TC
  • Journal Title: J Biol Chem Volume: 287(35) Pages: 29362-72
• [Journal Article] Dopamine D1-like Receptor-dependent Ectodomain Shedding and Release of Epidermal Growth Factor in Developing Striatal Neurons: (Part2) Target-Derived Neurotrophic Signaling (2011)
  • Author(s): Iwakura Y., Wang, R., Abe, Y., Piao, Y., Shishido,Y., Higashiyama, S., Takei, N.and Nawa, H
  • Journal Title: J Neurochem Volume: 118 Pages: 57-68
• [Journal Article] Qualitative and Quantitative Re-evaluation of Epidermal Growth Factor-ErbB1 Action on Developing Midbrain Dopamine Neurons in vivo nd in vitro; (Part 1) Target-Derived Neurotrophic Signaling (2011)
  • Author(s): wakura, Y., Zheng, Y., Abe, Y., Piao, Y., Yokomaku, D., Wang, R., Takei, N. and Nawa, H
  • Journal Title: J Neurochem Volume: 118 Pages: 45-46
• [Journal Article] Protein Production of an Active Neurotrophic Factor, Neuregulin-1; Qualitative Comparison of In Vitro Translation Systems (2011)
  • Author(s): Wang R, Iwakura Y, Araki K, Sotoyama H, Takei N and Nawa H
  • Journal Title: Neurosci Lett Volume: 497 Pages: 90-93
• [Journal Article] ctivation of mammalian target of rapamycin signaling in spatial learning (2010)
  • Author(s): Qi, S., Mizuno, M., Yonezawa, K., Nawa, H. and Takei, N
  • Journal Title: Neurosci. Res Volume: 68 Pages: 88-93
• [Journal Article] Downregulation of NF-kB signaling by N-terminal mutant huntingtin proteins induces oxdative stress and cell death (2010)
  • Author(s): Reijonen, S., Kukkonen, J.P., Kairisalo, M., Takei, N., Lindholm, D. and Korhonen, L
  • Journal Title: Cell Mol. Life Sci Volume: 67 Pages: 1929-194
• [Presentation] 神経細胞における翻訳調節:活動依存性と空間的特性 (2011)
  • Author(s): 武井延之、那波宏之
  • Organizer: 84回生化学会シンポジウム「翻訳制御の多様性」
  • Place of Presentation: 京都(オーガナイザー)
  • Year and Date: 2011-09-22
• [Book] 神経細胞特異的翻訳調節機構 (2012)
  • Author(s): 武井延之監修
  • Total Pages: 31