Functional analysis of the activatingfactor for endogenous neural stem cell migraion to develop the regeneration therapy for the injured brain.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500340
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Toho University
• Principal Investigator: HAMANOUE Makoto 東邦大学, 医学部, 講師 (00312025)
• Project Period (FY): 2010 – 2012
• Keywords: 膜透過型蛋白質 / りん酸化酵素 / 細胞遊走活性 / 損傷脳

Research Abstract

Cell-permeable p38 MAP kinase protein (Tat-p38) significantly enhances neural stem cell (NSC) migration,and also accumulates NSC into the brain legion. In addition, both of cell-permeable domain and kinase domain of Tat-p38 are required for this activity. These results suggest the potential of cell-permeable Tat-p38 protein for the regeneration therapy.

Research Products

• [Journal Article] Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons (2012)
  • Author(s): Kobayashi M, Hamanoue M, Masaki T, Furuta Y, Takamatsu K
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 429 Pages: 3-4,142-147
  • URL: http://dx.doi.org/10.1016/j.bbrc.2012.10.125
  • Peer Reviewed
• [Journal Article] Cell surface N-glycans mediated isolation of mouse neural stem cells (2011)
  • Author(s): Hamanoue M, Okano H
  • Journal Title: J Cell Physiology Volume: 226,6 Pages: 1433-1438
  • DOI: DOI:10.1002/jcp.22436
  • Peer Reviewed
• [Journal Article] Schwann Cell Plasticity After Spinal Cord Injury Shown by Neural Crest Lineage Tracing (2011)
  • Author(s): Nagoshi N, Shibata S, Hamanoue M, Mabuchi Y, Matsuzaki Y, Toyama Y, Nakamura M, Okano H
  • Journal Title: Glia Volume: 59 Pages: 771-784
  • DOI: DOI:10.1002/glia.211502012.11
  • Peer Reviewed
• [Presentation] 神経幹細胞に特異的に発現する糖転移酵素 (2013)
  • Author(s): 浜之上 誠, 池田 義孝, 高松 研
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-27
• [Presentation] 慢性脊髄損傷に対する内在性ミクログリア活性化療法の基礎的研究 (2012)
  • Author(s): 浜之上 誠, 森岡 和仁, 緒方 徹, 中嶋 一行, 高松 研
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Erk2 regulates proinflammatory gene expressions in demyelinating disorders.Neuroscience 2012 (2012)
  • Author(s): Okazaki R, Hayakawa K, Morioka K, Imamura O, Takishima K, Hamanoue M, Endo S, Tanaka S, Ogata T
  • Organizer: the 42th Annual SfN Meeting Neuroscience
  • Place of Presentation: New Orleans
  • Year and Date: 2012-10-17
• [Presentation] Secretion of growth factors from spinal cord microglia are enhanced by p38 MAP kinase (2012)
  • Author(s): Hamanoue M, Morioka K, Ogata T, Nakajima K, Takamatsu K
  • Organizer: Secretion of growth factors from spinal cord microglia are enhanced by p38 MAP kinase
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-15
• [Presentation] Binding profile and intracellular colocalization between hippocalcin and creatine kinase B subunit. The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry (2012)
  • Author(s): Kobayashi M, Hamanoue M, Takamatsu K
  • Organizer: The 55th Annual Meeting of the Japanese Society for Neurochemistry, The34th Annual Meeting of Japanese Society of Biological Phychiatry
  • Place of Presentation: Kobe International Conference Center
  • Year and Date: 2012-10-01
• [Presentation] p38 MAP kinase enhances secretion of GDNF and VEGF from rat spinal cord microglia. The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry, The 55th Annual Meeting of the Japanese Society for Neurochemistry (2012)
  • Author(s): Hamanoue M, Morioka K, Ogata T, Nakajima K, Takamatsu K
  • Organizer: The34th Annual Meeting of Japanese Society of Biological Phychiatry
  • Place of Presentation: Kobe International Conference Center
  • Year and Date: 2012-09-30
• [Presentation] 細胞外キナーゼによるミクログリア活性化機構の解析 (2012)
  • Author(s): 浜之上 誠, 中嶋 一行, 高松 研
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-30
• [Presentation] 内在性神経幹細胞遊走能活性化分子の機能解析 (2012)
  • Author(s): 浜之上 誠, 原科 純一
  • Organizer: 第139回東邦医学会例会
  • Place of Presentation: 東京
  • Year and Date: 2012-02-10
• [Presentation] The roles of n-acetylglucosaminyltransferase in adult mouse neural stem cells.Neuroscience 2011 (2011)
  • Author(s): Hamanoue M, Ikeda Y, Takamatsu K
  • Organizer: the 41th Annual SfN Meeting Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-11-15
• [Presentation] 神経幹細胞における糖転移酵素の発現と機能解析. Neuroscience2011 (2011)
  • Author(s): 浜之上 誠, 池田 義孝, 高松 研
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] 神経幹細胞内在性遊走能活性化分子の機能解析 (2011)
  • Author(s): 浜之上 誠, 水野 隆明, 岡野 ジェイムス洋尚, 芝田 晋介, 岡野 栄之, 高松 研
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-30
• [Presentation] ヒポカルシンと脳型クレアチンキナーゼ(CKB)の結合特性 (2011)
  • Author(s): 小林 正明, 浜之上 誠, 高松 研
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-30
• [Presentation] HIV-Tat-p38MAPキナーゼ蛋白質による神経幹細胞増殖誘導機構の解析 (2011)
  • Author(s): 浜之上 誠, 佐藤 健一郎
  • Organizer: 第137回東邦医学会例会
  • Place of Presentation: 東京
  • Year and Date: 2011-02-18
• [Presentation] ACE/ACE2-Angiotensin (1-7)-MAS Receptor Expression in Diabetic Nephropathy (2010)
  • Author(s): Mizuiri1 S, Hamanoue M, Hemmi H, Arita M, Aoki T, Ohashi Y, Sakai K, Shinozaki M, Shibuya K, Aikawa A
  • Organizer: ASN's 43rd Annual Meeting and Scientific Exposition
  • Place of Presentation: Denver, USA
  • Year and Date: 2010-11-20
• [Presentation] Protein transduction of Tat-p38 MAP kinase enhances adult neural progenitor cell migration. Neuroscience 2010 (2010)
  • Author(s): Hamanoue M, Okano HJ, Mizuno T, Shibata S, Okano H, Takamatsu K
  • Organizer: the 40th Annual SfN Meeting Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-14
• [Presentation] p38MAPキナーゼ直接導入による神経幹細胞遊走能活性化の解析 (2010)
  • Author(s): 浜之上 誠, 水野 隆明, 岡野 ジェイムス洋尚、岡野 栄之, 高松 研
  • Organizer: Neuro2010,第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] 脳型クレアチンキナーゼのカルシウム/ヒポカルシン依存性トランスロケーション (2010)
  • Author(s): 小林 正明, 浜之上 誠, 高松 研
  • Organizer: Neuro2010,第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] 糖尿病性腎症における腎ACE/ACE2、Ang1-7、Mas receptor (2010)
  • Author(s): 水入 苑生, 浜之上 誠, 篠崎 稔, 酒井 謙, 大橋 靖, 青木 敏行, 河村 毅, 渋谷 和俊, 相川 厚
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-17
• [Presentation] TAT-p38 MAPKタンパク質導入による神経幹細胞遊走能亢進作用 (2010)
  • Author(s): 浜之上 誠, 水野 隆明, 高松 研
  • Organizer: 第87回日本生理学大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-21
• [Presentation] ヒポカルシンを介した脳型クレアチンキナーゼのカルシウム依存性トランスロケーション (2010)
  • Author(s): 小林 正明, 浜之上 誠, 高松 研
  • Organizer: 第87回日本生理学大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-21
• [Remarks] 教育・研究業績データベース
  • URL: http://www.toho-u.ac.jp/kenkyu_db/index.html