2012 Fiscal Year Final Research Report
Regulation of neurotrophin secretion by exocytosis
| Project/Area Number |
22500344
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
MATSUDA Naoto 東京大学, 医科学研究所, 特任准教授 (40313100)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 神経科学 / 神経栄養因子 / 開口放出 |
|---|
| Research Abstract |
We aimed to establish experimental systems to observe BDNF secretion in living animals to elucidate the changes in brain functions associated with BDNF secretion. (1) We tried to express BDNF-pHluorin based on the zebrafish homolog in the visual system of living zebrafish larvae. However, we failed to observe significant fluorescence of BDNF-pHluorin. (2) We successfully expressed BDNF-pHluorin in the mouse hippocampus by lentivirus or electroporation, which enables us to investigate BDNF secretion in relation to memory formation.
|
