• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Functional study of Munc18 mutations associated with epilepsy

Research Project

  • PDF
Project/Area Number 22500353
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionSaitama University

Principal Investigator

ANDO Keiko  埼玉大学, 脳科学融合研究センター, 特任准教授 (40221741)

Co-Investigator(Kenkyū-buntansha) NAKAI Junichi  埼玉大学, 脳科学融合研究センター, 教授 (80237198)
OHKURA Masamichi  埼玉大学, 脳科学融合研究センター, 准教授 (70369172)
Project Period (FY) 2010 – 2012
Keywordsニューロン / シナプス / 神経回路 / 線虫
Research Abstract

To elucidate the molecular mechanism of intractable epilepsy, we developed C. elegans models having Munc18-1 mutations associated with Ohtahara syndrome (OS) known as early infantile epileptic encephalopathy (EIEE). We found that the Munc18-1 mutations resulted in behavioral defects such as paralysis and epileptic-like convulsions, and abnormalities in synaptic transmission. We also developed the optical measurement system of neural function using in vivo imaging and optogenetics. These disease models and optical technique can contribute to the understanding of the molecular pathogenesis and drug discovery for epilepsy.

  • Research Products

    (24 results)

All 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Genetically encoded green fluorescent Ca^<2+> indicators withimproved detectability for neuronal Ca^<2+> signals2012

    • Author(s)
      Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C,Ikegaya Y, Nakai J
    • Journal Title

      PLoS One

      Volume: 7(12) Pages: e51286

    • DOI

      doi:10.1371/journal.pone.0051286.

    • Peer Reviewed
  • [Journal Article] A Caenorhabditis elegansinsulin-like peptide, INS-17: its physiological function and expression pattern2012

    • Author(s)
      Matsunaga Y, Nakajima.K,Gengyo-Ando K, Mitani S, Iwasaki T, Kawano T
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 76(11) Pages: 2168-72

    • Peer Reviewed
  • [Journal Article] Neuronally expressed Ras-family GTPase Di-Ras modulates synaptic activity in Caenorhabditis elegans2012

    • Author(s)
      Tada M, Gengyo-Ando K, Kobayashi T, Fukuyama M, Mitani S, Kontani K, Katada T
    • Journal Title

      Genes Cells

      Volume: 17(9) Pages: 778-89

    • DOI

      doi:10.1111/j.1365-2443.2012.01627.x.

    • Peer Reviewed
  • [Journal Article] Depletion of mboa-7, anenzyme that incorporates polyunsaturated fatty acids into phosphatidylinositol (PI),impairs PI3-phosphate signaling in Caenorhabditis elegans.2012

    • Author(s)
      Lee HC, Kubo T, Kono N, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Inoue T, Arai H
    • Journal Title

      Genes Cells

      Volume: 17(9) Pages: 748-57

    • DOI

      doi:10.1111/j.1365-2443.2012.01624.x.

    • Peer Reviewed
  • [Journal Article] Physiological function, expression pattern, and transcriptional regulation of a Caenorhabditis elegans insulin-like peptide2012

    • Author(s)
      Matsunaga Y, Gengyo-Ando K, Mitani S, Iwasaki T, Kawano T
    • Journal Title

      INS-18. Biochem Biophys Res Commun

      Volume: 423(3) Pages: 478-83

    • DOI

      doi:10.1016/j.bbrc.

    • Peer Reviewed
  • [Journal Article] Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi2012

    • Author(s)
      Yoshina S, Sakaki K, Yonezumi-Hayashi A, Gengyo-Ando K, Inoue H, Iino Y, Mitani S
    • Journal Title

      Mol Biol Cell

      Volume: 23(9) Pages: 1728-41

    • DOI

      doi:10.1091/mbc.E11-10-0857.

    • Peer Reviewed
  • [Journal Article] GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans2012

    • Author(s)
      Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S,Gengyo-Ando K, Kage-Nakadai E, Mitani S, Nomura K
    • Journal Title

      Mol Biol Cell

      Volume: 23(6) Pages: 982-95

    • DOI

      doi:10.1091/mbc.E10-10-0855.

    • Peer Reviewed
  • [Journal Article] Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method2012

    • Author(s)
      Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, Yoshina S, Hori S, Mitani S
    • Journal Title

      BMC Biotechnol

      Volume: 12 Pages: 1

    • DOI

      doi:10.1186/1472-6750-12-1.

    • Peer Reviewed
  • [Journal Article] 香りの評価法の検討:バイオアッセイを用いた香り評価の試み2012

    • Author(s)
      中井淳一,永村ゆう子,大倉正道,清宮はるな,藤田孝,松永祐,長谷川登志夫,安藤恵子
    • Journal Title

      アロマリサーチ

      Volume: 13 Pages: 126-131

    • Peer Reviewed
  • [Journal Article] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division2011

    • Author(s)
      Nomura KH, Murata D, Hayashi Y, DejimaK, Mizuguchi S, Kage-Nakadai E,Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K
    • Journal Title

      Glycobiology

      Volume: 21(6) Pages: 834-48

    • DOI

      doi:10.1093/glycob/cwr019.

    • Peer Reviewed
  • [Journal Article] Multivesicular body formation requires OSBP-related proteins and cholesterol2010

    • Author(s)
      Kobuna H, Inoue T, Shibata M, Gengyo-Ando K, Yamamoto A, Mitani S, Arai H
    • Journal Title

      PLoS Genet

      Volume: 6(8)

    • DOI

      doi:pii:e1001055.10.1371/journal.pgen.1001055.

    • Peer Reviewed
  • [Journal Article] An Arf-like small G protein, ARL-8, promotes the axonal transport of presynapticcargoes by suppressing vesicle aggregation2010

    • Author(s)
      Klassen MP, Wu YE, Maeder CI, Nakae I, Cueva JG, Lehrman EK, Tada M,Gengyo-Ando K, Wang GJ, Goodman M, Mitani S, Kontani K, Katada T, Shen K
    • Journal Title

      Neuron

      Volume: 66(5) Pages: 710-23

    • DOI

      doi:10.1016/j.neuron.2010.04.033.

    • Peer Reviewed
  • [Journal Article] Two Golgi-resident3'-Phosphoadenosine5'-phosphosulfate transporters play distinct roles in heparansulfate modifications and embryonic and larval development in Caenorhabditis elegans2010

    • Author(s)
      Dejima K, Murata D, Mizuguchi S, Nomura KH, Izumikawa T, Kitagawa H, Gengyo-Ando K, Yoshina S, Ichimiya T, Nishihara S, Mitani S, Nomura K
    • Journal Title

      J Biol Chem

      Volume: 285 (32) Pages: 24717-28

    • DOI

      doi:10.1074/jbc.M109.088229.

    • Peer Reviewed
  • [Journal Article] The arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans2010

    • Author(s)
      Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T
    • Journal Title

      Mol Biol Cell

      Volume: 21(14) Pages: 2434-42

    • DOI

      doi:10.1091/mbc.E09-12-1010.

    • Peer Reviewed
  • [Journal Article] Protein phosphatase 2A cooperates with the autophagy-related kinase UNC-51 to regulate axon guidance in Caenorhabditis elegans2010

    • Author(s)
      Ogura K, Okada T, Mitani S,Gengyo-Ando K, Baillie DL, Kohara Y, Goshima Y
    • Journal Title

      Development

      Volume: 137(10) Pages: 1657-67

    • DOI

      doi:10.1242/dev.050708.

    • Peer Reviewed
  • [Journal Article] Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans2010

    • Author(s)
      Kage-Nakadai E, Kobuna H, Kimura M, Gengyo-Ando K, Inoue T, Arai H, Mitani S
    • Journal Title

      PLoS One

      Volume: 5(1) Pages: e8857

    • DOI

      doi:10.1371/journal.pone.0008857.

    • Peer Reviewed
  • [Journal Article] モデル生物線虫から見た低分子量G蛋白質Rab2010

    • Author(s)
      安藤恵子
    • Journal Title

      細胞

      Volume: 42(3) Pages: 104-107

  • [Presentation] Nakai J Functional analysis of de novoMunc18-1 mutations in epilepsy using Caenorhabditis elegans as a model organism2012

    • Author(s)
      Osawa A, Gengyo-Ando K
    • Organizer
      RIKEN BSI RETREAT 2012
    • Place of Presentation
      軽井沢
    • Year and Date
      20121112-13
  • [Presentation] Dynamic neuromuscular regulation in freely crawling C. elegans: multicellular Ca^<2+> imaging using GCaMPs2012

    • Author(s)
      Gengyo-Ando K, Usami A,Kagawa-Nagamura Y, Ohkura M, Ikegaya Y, Matsuki N, Nakai J
    • Organizer
      第35回神経 科学学会年会(招待講演)
    • Place of Presentation
      名古屋
    • Year and Date
      20120918-21
  • [Presentation] モデル動物線虫を利用したゲノム創薬2012

    • Author(s)
      安藤恵子
    • Organizer
      埼玉バイオIII研究交流会(招待講演)
    • Place of Presentation
      埼玉
    • Year and Date
      2012-03-21
  • [Presentation] Functional analysis of neuromuscular circuit using high-resolution in vivo Ca^<2+> imaging in C.elegans2011

    • Author(s)
      Gengyo-Ando K, Usami A,Kagawa-Nagamura Y, Yoshida Y, Matsuki N, Ikegaya Y,Nakai J
    • Organizer
      第34回日本分子生物学会年会(招待講演)
    • Place of Presentation
      横浜
    • Year and Date
      20111213-16
  • [Presentation] High-resolution in vivo Ca^<2+> imaging of neuromuscular system in Caenorhabditis elegans2011

    • Author(s)
      Gengyo-Ando K., Usami A.,Kagawa-Nagamura Y., Yoshida Y., Matsuki N., Ikegaya Y., and Nakai J
    • Organizer
      18th International C.elegans Meeting
    • Place of Presentation
      ロサンゼルス(アメリカ)
    • Year and Date
      20110622-26
  • [Presentation] Dynamic neuromuscularregulation in freely crawling C. elegans2011

    • Author(s)
      Usami A., Gengyo-Ando K., Nagamura Y., Yoshida Y., Matsuki N., Ikegaya Y. and Nakai J
    • Organizer
      high-resolution and large-scale in vivo Ca^<2+> imaging,Neuro 2011
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-16
  • [Remarks] 埼玉大学脳科学融合研究センター

    • URL

      http://subsi.saitama-u.ac.jp/

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi