2012 Fiscal Year Final Research Report
Synapse elimination and its molecular mechanisms of in vitro slice culture
| Project/Area Number |
22500359
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Teikyo University |
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Principal Investigator |
OHNO Takae 帝京大学, 医学部, 助教 (60508109)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 皮質脊髄路 / シナプス除去 / GluN2B / GluN2A / CaMKII / 臨界期 |
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| Research Abstract |
We previously showed in in vitro slice co-cultures of mice cerebral cortex and spinal cord that corticospinal (CS) synapses, once formed throughout the spinal cord, were eliminated from the ventral side during development in an NMDAR-dependent manner. This synapse elimination was regulated selectively by GluN2B (2B) and had critical period. In this study we first investigated whether this differential effect of 2B and 2A is due to difference in the amount of Ca2+ entry between these two types of subunits or to signaling molecules downstream to Ca2+ entry thereafter showed the involvement of CaMKII on the CS synapse elimination by using genetically modified mice. We further tried to identify the mechanism that closes the critical period and found that 2B decline is essential for closing the period.
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