2012 Fiscal Year Final Research Report
Study on regulation mechanisms of SR Ca2+-induced Ca2+ release by functional sub-domain of ryanodine receptor
| Project/Area Number |
22500366
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Fukuoka University |
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Principal Investigator |
UEHARA Akira 福岡大学, 医学部, 准教授 (60140745)
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| Co-Investigator(Kenkyū-buntansha) |
UEHARA Kiyoko 福岡大学, 医学部, 准教授 (00084244)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 筋生理学 |
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| Research Abstract |
The ryanodine receptors （RyR）release Ca2+ from SR in cardiac myocytes to trigger the muscle contraction. Our study aims at clarifying the structure-function relation of the cytoplasmic domain of the RyR molecule with ligand binding sites. We established experimental methods in which wild-type and mutant recombinant RyRs were expressed in HEK293 cells and single channel currents were recorded from the expressed RyR molecules. Identifying works regarding the amino acid regions of binding sites for ligands such as Ca2+, polyamine, calmodulin arenow in progress.
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