Analysis of deep microcirculation in cerebral cortex by Doppler optical coherence tomography

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fusional brain recording science
• Research Institution: Kansai University (2012); National Cardiovascular Center Research Institute (2010-2011)
• Principal Investigator: SEKI Junji 関西大学, 先端科学技術推進機構, 非常勤研究員 (20163082)
• Co-Investigator(Renkei-kenkyūsha): KOMAI Yutaka 大阪大学, 免疫学フロンティア研究センター, 准教授 (40443486)
• Project Period (FY): 2010 – 2012
• Keywords: ドップラOCT / 神経循環カップリング / 穿通枝血管 / 機能的カラム / 層構造 / 局所循環調節 / 1次体性感覚野 / 周波数領域OCT

Research Abstract

In order to visualize microvessels deeply embedded in the cortical tissue of brain, the Doppler frequency shift caused by blood flow is used as a marker to find out blood vessels in OCT signals. The 3-dimensional distribution of blood flow velocity obtained by Doppler OCT technique delineated penetrating microvessels as well as microvessels running deep in the cerebral cortex up to 1.5 mm depth. Penetrating arterioles were discriminated from venules based on the flow direction and pulsatility of blood flow. Electrical stimulus of hindpaw induced significant velocity increase in the penetrating arterioles (about 13% of the control value) and pial arterioles (10%) in the hindpaw area of primary somatosensory (S1) cortex, while the flow velocity did not change significantly in microvessels in the other area of S1 cortex. The information of structure and blood flow of microvessels in the direction of depth can help us to understand blood flow regulation among layers as well as functional columns of cerebral cortex.

Research Products

• [Journal Article] Infrared thermal imaging of rat somatosensory cortex with whisker stimulation (2012)
  • Author(s): Suzuki T., Ooi Y., Seki J
  • Journal Title: J. Appl. Physiol. Volume: 112 (7) Pages: 1215-22
  • DOI: DOI: 10.1152/japplphysiol.00867. 2011.
  • Peer Reviewed
• [Journal Article] Effects of autonomic nervous system on functional neuroimaging: analyses based on the vector autoregressive model (2012)
  • Author(s): Seiyama A., Sasaki Y., Takatsuki A., Seki J.
  • Journal Title: Adv. Exp. Med. Biol. Volume: 737 Pages: 77-82
  • DOI: DOI: 10.1007/978-1-4614- 1566-4_12.
  • Peer Reviewed
• [Journal Article] Temperature imaging by infrared measurement in rat somatosensory cortex with whisker stimulation (2011)
  • Author(s): Suzuki T., Seki J., Ooi Y.
  • Journal Title: Proc. 9th World Congress for Microcirculation, Medimond Pages: 75-79
• [Journal Article] Visualization of microvessels deeply embedded in cerebral cortex of rats by Doppler optical coherence tomography (2011)
  • Author(s): Seki J., Suzuki T., Ooi Y.
  • Journal Title: Proc. 9th World Congress for Microcirculation, Medimond Pages: 59-63
• [Presentation] マイクロチャネル内流れ中の血小板模擬粒子の運動 (2013)
  • Author(s): 田中慎之介、関淳二、板野智昭、関眞佐子
  • Organizer: 第36回日本バイオレオロジー学会
  • Place of Presentation: 福岡
  • Year and Date: 2013-06-08
• [Presentation] Brain temperature and brain energy changes during tasks and light anesthesia: estimation with MRS (2013)
  • Author(s): Yoshioka Y., Oikawa H., Kanbara Y., Matsumura Y., Inoue T., Shinohe Y., Joh S., Matsuda T., Nabetani A., Seki J.
  • Organizer: 国際磁気共鳴医学会ISMRM
  • Place of Presentation: Salt Lake City, USA
  • Year and Date: 2013-04-25
• [Presentation] Fundamental researches on measurement of the blood glucose concentration using the polarimetric technique (2012)
  • Author(s): Seiyama A., Suekane A., Miura T., Seki J.
  • Organizer: International Society of Oxygen Transport to Tissue
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2012-08-21
• [Presentation] ドップラOCT法によるラット脳微小循環の可視化 (2011)
  • Author(s): 関淳二、鈴木崇士、大井康浩
  • Organizer: 第34回日本バイオレオロジー学会
  • Place of Presentation: 吹田
  • Year and Date: 2011-06-04
• [Presentation] Doppler optical coherence tomography revealed the structure and function of penetrating microvessels of rat cerebral cortex in vivo (2011)
  • Author(s): 関淳二、鈴木崇士、大井康浩
  • Organizer: 第36回日本微小循環学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-02-12
• [Presentation] Temperature imaging of neural activity in rat brain evoked by whisker stimulation (2011)
  • Author(s): 鈴木崇士、大井康浩、関淳二
  • Organizer: 第36回日本微小循環学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-02-12
• [Presentation] Visualization of microvessels deeply embedded in cerebral cortex of rats by Doppler optical coherence tomography (2010)
  • Author(s): Seki J., Suzuki T., Ooi Y.
  • Organizer: 9th World Congress for Microcirculation
  • Place of Presentation: Paris, France
  • Year and Date: 2010-09-28
• [Presentation] Temperature imaging by infrared measurement in rat somatosensory cortex with whisker stimulation (2010)
  • Author(s): Suzuki T., Seki J., Ooi Y.
  • Organizer: 9th World Congress for Microcirculation
  • Place of Presentation: Paris, France
  • Year and Date: 2010-09-26
• [Presentation] Visualization of microvessels deeply embedded in the rat cerebral cortex by Doppler OCT technique (2010)
  • Author(s): Seki J., Suzuki T., Ooi Y.
  • Organizer: 第49回日本生体医工学会
  • Place of Presentation: 大阪国際交流センター、大阪
  • Year and Date: 2010-06-27
• [Presentation] Visualization of deep microvessels in cerebral cortex by optical coherence tomography (2010)
  • Author(s): Seki J., Suzuki T., Ooi Y.
  • Organizer: 2010 KSBB Spring Meeting and International Symposium, Kyungwon University
  • Place of Presentation: Seongnam, Korea
  • Year and Date: 2010-04-16