2012 Fiscal Year Final Research Report

Identification of genes responsible for resistance/susceptibility toSendai virus infection in the strain difference in mice.

Research Project

Project/Area Number	22500381
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Laboratory animal science
Research Institution	Hokkaido University
Principal Investigator	AGUI Takashi 北海道大学, 大学院・獣医学研究科, 教授 (00212457)
Project Period (FY)	2010 – 2012
Keywords	センダイウイルス / マウス / 感染抵抗性因子 / コンジェニックマウス / QTL
Research Abstract	It is known that there is strain difference in the laboratory mice in the resistance/susceptibility to Sendai virus infection. It is, further, known that three QTLs are identified to be responsible for the resistance/susceptibility to Sendai virus infection, based on the QTL analysis using C57BL/6 (B6) and DBA/2 (D2) mice as a representative of the resistant and susceptible strains, respectively. In this research, we produced 6 congenic strains, in which 3 QTLS were introgressed into B6 and D2 mice, respectively. Further, we produced 2 congenic strains, in which 2 QTLs showing epistatic interaction were simultaneously introgressed into B6 and D2 mice, respectively. Using these 8 congenic strains, we conducted infection experiments with Sendai virus. We obtained results that these three introgressed QTLs invested resistance to D2 mice, thus confirming that genes responsible for resistance to Sendai virus infection should exist in these three QTLs.