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2013 Fiscal Year Final Research Report

In vitro induction and in vivo transplantation of the pancreatic organ using the undifferentiated cells of Xenopus embryo.

Research Project

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Project/Area Number 22500385
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionTamagawa University (2011-2013)
The University of Tokyo (2010)

Principal Investigator

ARIIZUMI Takashi  玉川大学, 農学部, 教授 (30286166)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Shuji  広島大学, 理学研究科, 特任准教授 (90447318)
Project Period (FY) 2010-04-01 – 2014-03-31
Keywords発生工学 / 器官創製 / 膵臓 / 未分化細胞 / 生体移植
Research Abstract

We attempted to develop a reliable experimental system for in vitro pancreas induction based on the dissociation/reaggregation protocol previously reported. Additional treatment of the reaggregates with retinoic acid after the activin induction dramatically changed their gene expression and histo-differentiation patterns from heart to pancreas. Next, we tried to prove that the in vitro-induced pancreatic primordium can develop a structural and functional pancreas by the in vivo ectopic transplantation. The ectopic pancreas showed the same morphological features as to the normal functioning pancreas composed of the exocrine and endocrine portions. Moreover, the fact that the ectopic pancreas actually regulates blood glucose level was proved by the extirpation experiment and the glucose tolerance test.

  • Research Products

    (13 results)

All 2013 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (5 results)

  • [Journal Article] アクチビンと臓器形成2013

    • Author(s)
      有泉高史
    • Journal Title

      東京都生物教育研究会

      Volume: 1 Pages: 112-116

  • [Journal Article] Hippo signaling components, Mst1 and Mst2, act as a switch between self-renewal and differentiation in Xenopus hematopoietic and endothelial progenitors2013

    • Author(s)
      Nejigane S, Takahashi S, Haramoto Y, Michiue T, Asashima M
    • Journal Title

      Int J Dev Biol

      Volume: 57 Pages: 407-414

    • DOI

      10.1387/ijdb.130010st

    • Peer Reviewed
  • [Journal Article] Xnr3 affects brain patterning via cell migration in the neural-epidermal tissue boundary during early Xenopus embryogenesis2013

    • Author(s)
      Morita M, Yamashita S, Matsukawa S, Haramoto Y, Takahashi S, Asashima M, Michiue T
    • Journal Title

      Int J Dev Biol

      Volume: 57 Pages: 779-786

    • DOI

      10.1387/ijdb.130161tm

    • Peer Reviewed
  • [Journal Article] Development of Ca²(+) signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation2011

    • Author(s)
      Takano K, Obata S, Komazaki S, Masumoto M, Oinuma T, Ito Y, Ariizumi T, Nakamura H, Asashima M
    • Journal Title

      Dev Growth Differ

      Volume: 53 Pages: 37-47

    • DOI

      10.1111/j.1440-169X.2010.01220.x

    • Peer Reviewed
  • [Journal Article] The transcriptional coactivators Yap and TAZ are expressed during early Xenopus development2011

    • Author(s)
      Nejigane S, Takahashi S, Haramoto Y, Michiue T, Asashima M
    • Journal Title

      Int J Dev Biol

      Volume: 55 Pages: 121-126

    • DOI

      10.1387/ijdb.103130sn.2011

    • Peer Reviewed
  • [Journal Article] Creating frog heart as an organ : in vitro-induced heart functions as a circulatory organ in vivo2010

    • Author(s)
      Kinoshita M, Ariizumi T, Yuasa S, Miyoshi S, Komazaki S, Fukuda K, Asashima M
    • Journal Title

      Int J Dev Biol

      Volume: 54 Pages: 851-856

    • DOI

      10.1387/ijdb.093036mk.2010

    • Peer Reviewed
  • [Journal Article] Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation2010

    • Author(s)
      Yamagishi M, Ito Y, Ariizumi T, Komazaki S, Danno H, Michiue T, Asashima M
    • Journal Title

      Dev Growth Differ

      Volume: 52 Pages: 665-75

    • DOI

      10.1111/j.1440-169X.2010.01204.x.2010

    • Peer Reviewed
  • [Journal Article] Reduction of N-glycolylneuraminic acid in human induced pluripotent stem cells generated or cultured under feeder- and serum-free defined conditions2010

    • Author(s)
      Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M
    • Journal Title

      PLoS One

      Volume: 5 Pages: e14099

    • DOI

      10.1371/journal.pone.0014099.2010

    • Peer Reviewed
  • [Presentation] 変態促進させたアホロートルの四肢再生2013

    • Author(s)
      三浦颯人, 田川日奈子, 有泉高史
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-26
  • [Presentation] アクチビンと臓器形成2013

    • Author(s)
      有泉高史
    • Organizer
      日本生物教育会第68回大会
    • Place of Presentation
      東京
    • Year and Date
      2013-08-08
  • [Presentation] Different cellular contribution of somite-derived tissues to limb regeneration in larval and adult Xenopus2013

    • Author(s)
      永山誓史, 有泉高史, 田村宏治, 横山仁
    • Organizer
      日本発生生物学会第46回大会(APDBN共催)
    • Place of Presentation
      島根
    • Year and Date
      2013-05-31
  • [Presentation] アフリカツメガエルの四肢再生における体節由来組織の寄与について2012

    • Author(s)
      永山誓史, 有泉高史, 横山仁, 田村宏治
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-15
  • [Presentation] ツメガエル胚のアニマルキャップから分化誘導した膵臓の血糖調節能2011

    • Author(s)
      有泉高史, セン徳川, 高橋秀治, 浅島誠
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21

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Published: 2015-07-16  

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