Technical maintenance to establish a rabbit sperm bank for human disease models

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500386
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Saga University
• Principal Investigator: KITAJIMA Shuji 佐賀大学, 総合分析実験センター, 准教授 (70284643)
• Co-Investigator(Kenkyū-buntansha): NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874)
• Co-Investigator(Renkei-kenkyūsha): NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874) ; FAN Jianglin 山梨大学, 医学工学総合研究部, 教授 (60272192)
• Project Period (FY): 2010 – 2012
• Keywords: 疾患モデル / 遺伝子改変ウサギ

Research Abstract

We developed and improved protocols of rabbit semen cryopreservation and artificial insemination, these protocols are important to establish a rabbit sperm bank of human disease models. We completed cryopreservation of six genetically modified rabbits by frozen semen and made public information of these rabbits on the web site.

Research Products

• [Journal Article] Delaying embryo development by storing at 4°C for synchronization to recipients in microinjection technique in rabbits (2013)
  • Author(s): Nishijima, K., Liu, E., Yamaguchi, S., Tanaka, M., Morimoto, M., Watanabe, T., Fan, J. and Kitajima, S.
  • Journal Title: Lab. Anim Volume: 47 Pages: 53-57
  • DOI: DOI:10.1258/la.2012.012097
  • Peer Reviewed
• [Journal Article] Human apolipoprotein AII protects against diet-induced atherosclerosis in transgenic rabbits (2013)
  • Author(s): Wang, Y., Niimi, M., Nishijima, K., Yu, Y., Koike, T., Kitajima, S., Inoue, T., Waqar, A.B., Liu, E., Kohashi, M., Keyamura, Y., Yoshigawa, T., Zhang, J., Ma, L., Zha, X., Watanabe, T., Asada, A., Y. Chen, E. and Fan, J.
  • Journal Title: Arterioscler. Thromb. Vasc. Biol Volume: 33 Pages: 224-231
  • DOI: DOI:10.1161/ATVBAHA.112.300445
  • Peer Reviewed
• [Journal Article] Genetic characterization of four wild species of Chinese marmots using microsatellite markers (2012)
  • Author(s): Chen, Y., Wang, Z., Zhang, G., Fan, W., Tao, Y., He, W., Zhao, S., Hung, B., Fan, J., Kitajima, S., Liu, E.
  • Journal Title: Biologia Volume: 67(5) Pages: 1013-1017
  • DOI: DOI:10.2478/s11756-012-0088-8
  • Peer Reviewed
• [Journal Article] Life span of Common Marmoset (Callithrix jacchus) at CLEA Japan breeding colony (2012)
  • Author(s): Nishijima, K., Saitoh, R., Tanaka, S., Ohsato-Suzuki, M., Ohno, T., Kitajima, S.
  • Journal Title: Biogerontol. Volume: 13(4) Pages: 439-443
  • DOI: DOI:10.1007/s10522-012-9388-1
  • Peer Reviewed
• [Journal Article] Effect of the primary cooling rate on the motility and fertility of the frozen-thawed rabbit spermatozoa (2012)
  • Author(s): Maeda, T., Liu, E., Nishijima, K., Tanaka, M., Yamaguchi, S., Morimoto, M., Watanabe, T., Fan, J. and Kitajima, S.
  • Journal Title: World Rabbit Sci. Volume: 20 Pages: 65-70
  • DOI: DOI:10.4995/wrs.2012.1080
  • Peer Reviewed
• [Journal Article] Endothelial lipase mediates HDL levels in normal and hyperlipidemic rabbits (2012)
  • Author(s): Zhang, J., Ying Yu, Y., Nakamura, K., Tomonari Koike, T., Waqar, A. B., Zhang, X., Liu, E., Nishijima, K., Kitajima, S., Shiomi, M., Qi, Z., Yu, J., Graham, M. J., Crooke, R. M., Ishida, T., Hirata, K., Hurt-Camejo, E., Chen, Y. E., Fan, J.
  • Journal Title: J. Atheros. Thromb Volume: 19(3) Pages: 213-226
  • DOI: DOI:10.5551/jat.11148
  • Peer Reviewed
• [Journal Article] Age is an important factor influence on the number of recovered eggs in superovulated rabbits: Analysis of our past 5 years data of 509 rabbits superovulated by FSH or PMSG. Scand (2011)
  • Author(s): Kitajima, S., Maeda, T., Liu, E., Nishijima, K., Morimoto, M., Watanabe, T. and Fan, J.
  • Journal Title: J. Lab. Anim. Sci. Volume: 38(3) Pages: 169-174
  • URL: http://biomedicum.ut.ee/sjlas/38_3_169-174.pdf
  • Peer Reviewed
• [Journal Article] Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits (2011)
  • Author(s): Matsuda, S., Yamashita, A., Sato, Y., Kitajima, S., Koike, T., Sugita, C., Moriguchi-Goto, S., Hatakeyama, K., Takahashi, M., Koshimoto, C., Matsuura, Y., Iwakiri, T., Chen, Y.E., Fan, J. and Asada. Y.
  • Journal Title: Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits Volume: 9 Pages: 201-208
  • DOI: DOI:10.1111/j.1538-7836.2010.
  • Peer Reviewed
• [Journal Article] ウサギ精子・胚の凍結保存とバイオリソース (2010)
  • Author(s): 北嶋修司,西島和俊
  • Journal Title: アニテックス Volume: 22(3) Pages: 32-37
• [Journal Article] 医学研究における遺伝子改変ウサギの応用とその展望 (2010)
  • Author(s): 範 江林,小池智也,西島和俊,北嶋修司
  • Journal Title: アニテックス Volume: 22(3) Pages: 11-15
• [Journal Article] 遺伝子改変ウサギの系統維持のための精子凍結保存の有用性に関する検討:約5.6年間凍結保存された遺伝子組換えウサギ由来精子を用いた人工授精成績 (2010)
  • Author(s): 西島和俊,山口慎二,森本正敏,渡辺照男,北嶋修司
  • Journal Title: 九州実験動物雑誌 Volume: 19 Pages: 35-39
  • URL: http://www.miyazaki-med.ac.jp/AnimalCenter/11/26.html
  • Peer Reviewed
• [Journal Article] Overexpression of peroxiredoxin 4 protects against high-dose streptozotocin-induced diabetes by suppressing oxidative stress and cytokines in transgenic mice (2010)
  • Author(s): Ding, Y., Yamada, S., Wang, K.Y., Shimajiri, S., Guo, X., Tanimoto, A., Murata, Y., Kitajima, S., Watanabe, T., Izumi, H., Kohno, K., Sasaguri, Y.
  • Journal Title: Antioxid. Redox Signal Volume: 13(10) Pages: 1477-1490
  • DOI: DOI:10.1089/ars.2010.3137
  • Peer Reviewed
• [Presentation] ウサギの精子および胚凍結における不凍タンパク質の効果 (2013)
  • Author(s): 西島和俊、田中麻衣、酒井悠輔、越本知大、森本正敏、渡辺照男、範 江林、北嶋修司
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 筑波
  • Year and Date: 20130515-17
• [Presentation] ヒトアポリポ蛋白A-IIは動脈硬化を抑制する:新しいトランスジェニックウサギモデルの開発及び応用 (2013)
  • Author(s): 王 瑶、新見 学、西島和俊、Ahmed Bilal Waqar、于 瑩、小池智也、北嶋修司、範 江林
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 筑波
  • Year and Date: 20130515-17
• [Presentation] 遺伝子改変ウサギの開発と維持-医学研究への利用 (2013)
  • Author(s): 北嶋修司、西島和俊
  • Organizer: JALAMシンポジウム
  • Place of Presentation: 筑波
  • Year and Date: 2013-05-14
• [Presentation] Cholesterol-loaded cyclodextrinを添加したウサギ精子の凍結融解後の運動性 (2012)
  • Author(s): 田中麻衣、西島和俊、酒井悠輔、森本正敏、越本知大、渡辺照男、北嶋修司
  • Organizer: 第30回九州実験動物研究会総会
  • Place of Presentation: 長崎
  • Year and Date: 20121110-11
• [Presentation] ウサギバイオリソース整備に向けての試み (2012)
  • Author(s): 北嶋修司
  • Organizer: 公開シンポジウム「先進医療研究に貢献するウサギ疾患モデルの開発と動脈硬化の病態解明への応用」
  • Place of Presentation: 山梨
  • Year and Date: 2012-08-11
• [Presentation] ヒトC-反応性蛋白遺伝子導入ウサギの骨代謝 (2012)
  • Author(s): 西島和俊、増山律子、田中麻衣、森本正敏、渡辺照男、範 江林、北嶋修司
  • Organizer: 第1回ウサギバイオサイエンス研究会
  • Place of Presentation: 宮崎
  • Year and Date: 2012-08-04
• [Presentation] Apolipoprotein A-II plays an important role in lipid metabolism (2012)
  • Author(s): Koike, T., Kitajima, S., Nishijima, K., Liu, E., Teruo Watanabe, T., Fan, J.
  • Organizer: 第1回 日中合同ウサギバイオサイエンスフォーラム
  • Place of Presentation: 宮崎
  • Year and Date: 2012-08-04
• [Presentation] Assessment of energy expenditure in rabbit with doubly-labeled water method (2011)
  • Author(s): Nishijima, K., Yamada, Y., Tanaka, H., Kitajima, S., Yamaguchi, S., Morimoto, M., Tanaka, K., Nishida, Y.
  • Organizer: he 4th International Meeting on Rabbit Biotechnology
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 20110730-0601
• [Presentation] Apolipoprotein-AII plays an important role in lipid metabolism and atherosclerosis (2011)
  • Author(s): Koike, T., Kitajima, S., Nishijima, K., Liu, E., Watanabe, T., Fan, J.
  • Organizer: The 4th International Meeting on Rabbit Biotechnology
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 20110730-0601
• [Presentation] Cholesterol loaded cyclodextrin の添加はウサギの凍結融解後精子の運動率を高める (2011)
  • Author(s): 西島和俊、山口慎二、森本正敏、渡辺照男、北嶋修司
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110525-27
• [Presentation] ウサギ精子凍結融解後の運動率に及ぼすCholesterol-loaded cyclodextrin添加の影響 (2011)
  • Author(s): 山口慎二、西島和俊、森本正敏、渡辺照男、北嶋修司
  • Organizer: 第29回九州実験動物研究会総会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-11-12
• [Presentation] Human C-reactive Protein Enhances Thrombus Formation In Transgenic Rabbits,(Core 7. Vascular Disease: Biology and Clinical Science, Session Title: Sol Sherry Distinguished Lecture in Thrombosis: New Mechanisms and New Targets in Thrombosis, Abstract 12227) (2010)
  • Author(s): Matsuda, S., Yamashita, A., Kitajima, S., Fan, J., Asada, Y.
  • Organizer: AHA Scientific Session
  • Place of Presentation: Chicago, USA
  • Year and Date: 20101113-17
• [Presentation] Human C-reactive protein enhances thrombus formation in transgenic rabbits (2010)
  • Author(s): Matsuda, S., Kitajima, S., Koike, T., Fan, J. and Asada, J.
  • Organizer: The 42nd Annual Scientific Meeting of the Japanese Atherosclerosis Society
  • Place of Presentation: Gifu
  • Year and Date: 20100715-16
• [Presentation] Expression of Human ApoAII in Transgenic Rabbits Leads to Dyslipidemia -A New Model for Combined Hyperlipidemia (2010)
  • Author(s): Koike, T., Kitajima, S., Yu, Y., Nishijima, K., Waqar, AB., Inoue, T., Wang, Y., Zhang, B., Kobayashi, J., Masatoshi, M., Saku, K., Watanabe, T. and Fan, J.
  • Organizer: The 42nd Annual Scientific Meeting of the Japanese Atherosclerosis Society
  • Place of Presentation: Gifu
  • Year and Date: 20100715-16
• [Presentation] ウサギの大腿骨特性について (2010)
  • Author(s): 田中 愼,西島和俊,大野民生、北嶋修司
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都
  • Year and Date: 20100514-16
• [Presentation] 佐賀大学におけるヒト疾患モデルとしての遺伝子改変ウサギの開発と保存状況について (2010)
  • Author(s): 北嶋修司,西島和俊,劉 恩岐、小池智也、森本正敏,渡辺照男,範 江林
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都
  • Year and Date: 20100514-16
• [Presentation] 西島和俊、小池智也、山口慎二、森本正敏、渡辺照男、範 江林,北嶋修司 (2010)
  • Author(s): 西島和俊、小池智也、山口慎二、森本正敏、渡辺照男、範 江林,北嶋修司
  • Organizer: 第28回九州実験動物研究会総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-23
• [Presentation] ウサギ凍結精子を用いた人工授精成績の向上に関する検討 (2010)
  • Author(s): 山口慎二、前田達弘、西島和俊、森本正敏、北嶋修司
  • Organizer: 第28回九州実験動物研究会総会
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-23
• [Presentation] 受精卵移植による秋田大型ウサギのSPF化 (2010)
  • Author(s): 松田幸久、稲垣秀晃、柴田淑子、北嶋修司、西島和俊
  • Organizer: 第4回ウサギフォーラム
  • Place of Presentation: 秋田
  • Year and Date: 2010-07-24
• [Presentation] 長期保存された遺伝子改変ウサギ凍結精子の受精能の検討 (2010)
  • Author(s): 西島和俊、山口慎二、森本正敏、渡辺照男、北嶋修司
  • Organizer: 第4回ウサギフォーラム
  • Place of Presentation: 秋田
  • Year and Date: 2010-07-24
• [Presentation] ヒト C 反応性蛋白は心血管疾患における血栓形成に影響を与えるか? 遺伝子 改変ウサギを用いた動脈硬化性血栓形成の検討 (2010)
  • Author(s): 松田俊太郎、山下 篤、北嶋修司,小池智也、範 江林、浅田祐士郎
  • Organizer: 第4回ウサギフォーラム
  • Place of Presentation: 秋田
  • Year and Date: 2010-07-24
• [Remarks]
  • URL: http://www.animal.med.saga-u.ac.jp/index.php?id=2