2012 Fiscal Year Final Research Report
Freeze preservation for reproductive tissue on Three Dimensional Measurement of cryprotectant and ice crystal
| Project/Area Number |
22500393
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nihon University |
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Principal Investigator |
都 甲洙 日本大学, 生物資源科学部, 准教授 (40385993)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kahei 日本大学, 生物資源科学部, 教授 (20059778)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 凍結保存 |
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| Research Abstract |
The cryoprotectant is commonly used to protect reproductive tissues during freezing and thawing. As an effort to investigate the osmotic behavior of cryoprotectant, near-infrared (NIR) techniques were applied to monitor the concentration of ethylene glycol (EG) within reproductive tissues. The proposed method provided a novel tool to investigate the optimal freezing method of reproductive tissues on cryoprotectant, ice crystals and cell destruction.
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