• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Immunosensor with Fluid Control Mechanism for Salivary Cortisol Analysis

Research Project

  • PDF
Project/Area Number 22500400
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionIwate University

Principal Investigator

YAMAGUCHI Masaki  岩手大学, 工学部, 教授 (50272638)

Project Period (FY) 2010 – 2012
Keywords計測工学 / 生物・生体工学 / 分子認識 / 流体工学 / ストレス
Research Abstract

The clinical utility of salivary cortisol measurements has been restricted by the lack of appropriate technology platforms that allow near real-time" detection and quantification of salivary cortisol. Integrating early identification and risk stratification in patients with stress-related diseases will require that care providers have timely access to salivary stress biomarker data. The purpose of this research is to demonstrate a new design for a cortisol immunosensor for the noninvasive and quantitative analysis of salivary cortisol. We propose a cortisol immunosensor with a fluid control mechanism which has both a vertical flow and a lateral flow. The detected current resulting from a competitive reaction between the sample cortisol and a glucose oxidase-labeled cortisol conjugate was found to be inversely related to the concentration of cortisol in the sample solution. The measurement could be accomplished within 35 minutes and the cortisol immunosensor could be reused. Additionally, a novel saliva collection kit that can fix the saliva volume with only a four-step action was proposed. Used for evaluation of human salivary cortisol levels, the cortisol immunosensor measurement corresponded closely with commercially available ELISA method R^2 = 0.92) for a range of the concentrations of native salivary cortisol (0.1 . 10 ng/ml). Our results indicate the promise of the new cortisol immunosensor for noninvasive, point-of care measurement of human salivary cortisol levels.

  • Research Products

    (39 results)

All 2013 2012 2011 2010 Other

All Journal Article (18 results) (of which Peer Reviewed: 13 results) Presentation (13 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] 震災復興に貢献する唾液コルチゾール測定器の開発2013

    • Author(s)
      山口 昌樹,丹羽 大介
    • Journal Title

      機能材料

      Volume: Vol.33, No.1 Pages: 57-61

  • [Journal Article] 唾液ストレスマ-カ-と非侵襲的診断2012

    • Author(s)
      山口 昌樹
    • Journal Title

      Bio Industry

      Volume: Vol.29, No.10 Pages: 25-30

  • [Journal Article] Immunosensor with Fluid Control Mechanism for Salivary Cortisol Analysis2012

    • Author(s)
      Masaki Yamaguchi, Yohei Matsuda, Shohei Sasaki, Makoto Sasaki, Yoshihiro Kadoma, Yoshikatsu Imai, Daisuke Niwa, Vivek Shetty
    • Journal Title

      Biosensors & Bioelectronics

      Volume: Vol.41 Pages: 186-191

    • Peer Reviewed
  • [Journal Article] Utility of a Salivary Biosensor for Objective Assessment of Surgery-Related Stress2012

    • Author(s)
      Theodore F. Robles, Rassilee Sharma, Kwan-Soo Park, Lauren Harrell, Masaki Yamaguchi, and Vivek Shetty
    • Journal Title

      Journal of Oral and Maxillofacial Surgery

      Volume: Vol.70, No.10 Pages: 2256-2263

    • Peer Reviewed
  • [Journal Article] Noninvasive Evaluation of the Chronic Influence of Local Air Velocity from an Air Conditioner using Salivary Cortisol and Skin Caspase-14 as Biomarkers of Psychosomatic and Environmental Stress2012

    • Author(s)
      M Yamaguchi and H Nishimiya
    • Journal Title

      The Journal of International Medical Research

      Volume: Vol.40, No.4 Pages: 1429-1437

    • Peer Reviewed
  • [Journal Article] 唾液アミラーゼ活性を用いた月経周期に伴う女性のストレス評価2012

    • Author(s)
      中川 朝美,佐々木 誠,山口 昌樹
    • Journal Title

      ライフサポート

      Volume: Vol.24, No.3 Pages: 123-127

    • Peer Reviewed
  • [Journal Article] Patterns of Salivary Cortisol Levels Can Manifest Work Stress in Emergency Care Providers2012

    • Author(s)
      Yasushi Nakajima, Takayuki Takahashi, Vivek Shetty, Masaki Yamaguchi
    • Journal Title

      The Journal of Physiological Sciences

      Volume: Vol.62, No.3 Pages: 191-197

    • Peer Reviewed
  • [Journal Article] 唾液バイオマーカーによる精油の嗜好と生理反応の関連性の検証2012

    • Author(s)
      中野 敦行,長山 優,佐々木 誠,山口 昌樹
    • Journal Title

      アロマテラピー学雑誌

      Volume: Vol. 12, No.1 Pages: 34-40

    • Peer Reviewed
  • [Journal Article] An Analytical Technique for Measuring Protein Carbonyl in the Stratum Corneum Using Surface Plasmon Resonance2012

    • Author(s)
      Akira Date, Tomoki Shimakura, Makoto Sasaki, and Masaki Yamaguchi
    • Journal Title

      International Journal of Cosmetic Science

      Volume: Vol.34 Pages: 81-85

    • Peer Reviewed
  • [Journal Article] バイオマスプラスチックの医療用具への応用に向けて2011

    • Author(s)
      山口 昌樹
    • Journal Title

      プラスチックス

      Volume: Vol.62, No.6 Pages: 1-4

  • [Journal Article] Salivary Sensors for Quantification of Stress Response Biomarker2011

    • Author(s)
      Masaki YAMAGUCHI and Vivek SHETTY
    • Journal Title

      Electrochemistry

      Volume: Vol.79, No.6 Pages: 442-446

  • [Journal Article] 唾液アミラーゼによるストレスの評価2011

    • Author(s)
      中野 敦行,山口 昌樹
    • Journal Title

      バイオフィードバック研究

      Volume: Vol.38, No.1 Pages: 3-9

  • [Journal Article] 救急医療従事者の夜間勤務が唾液アミラーゼ活性の日内変動へもたらす影響2011

    • Author(s)
      中島 康,高橋 貴之,山口 昌樹
    • Journal Title

      日本救急医学会雑誌

      Volume: Vol. 22, No.3 Pages: 117-124

    • Peer Reviewed
  • [Journal Article] Developmental validation of a point-of-care, salivary α-amylase biosensor2011

    • Author(s)
      Vivek Shetty, Corwin Zigler, Theodore F. Robles, David Elashoff, Masaki Yamaguchi
    • Journal Title

      Psychoneuroendocrinology

      Volume: Vol.36, No.2 Pages: 193-199

    • Peer Reviewed
  • [Journal Article] Effect of Facial Expression of Mother on 15-21-Month-Old Infants Using Salivary Biomarker2011

    • Author(s)
      Akiko Tange, Megumi Mino, Kiyoshi Miyazawa, Kazuo Hiraki, Syohji Itakura and Masaki Yamaguchi
    • Journal Title

      Sensors and Materials

      Volume: Vol. 23, No.1 Pages: 87-94

    • Peer Reviewed
  • [Journal Article] The feasibility of ambulatory biosensor measurement of salivary alpha amylase: Relationships with self-reported and naturalistic psychological stress2011

    • Author(s)
      Theodore F. Robles, Vivek Shetty, Corwin M. Zigler, Dorie A. Glover, David Elashoff, Debra Murphy, Masaki Yamaguchi
    • Journal Title

      Biological Psychology

      Volume: Vol. 86, No.1 Pages: 50-56

    • Peer Reviewed
  • [Journal Article] Immunochromatographic cortisol biosensor using enzyme-labeled conjugate2010

    • Author(s)
      Yusuke Tahara, Suguru Yoshikawa, Vivek Shetty, and Masaki Yamaguchi
    • Journal Title

      Sensors and Materials

      Volume: Vol. 22, No.8 Pages: 427-437

    • Peer Reviewed
  • [Journal Article] Influence of local air velocity from air conditioner evaluated by salivary and skin biomarkers2010

    • Author(s)
      Masaki Yamaguchi, Takayuki Takahashi, Yuichiro Yoshino, Makoto Sasaki, Hajime Nishimiya
    • Journal Title

      Building & Environment

      Volume: Vol.45, No.11 Pages: 2539-2544

    • Peer Reviewed
  • [Presentation] ホルモン分析の自動化を目指した唾液採取器具の提案2012

    • Author(s)
      手塚 勇希,佐々木 翔平,松田 陽平,今井 義勝,丹羽 大介,佐々木 誠,山口 昌樹
    • Organizer
      第46回日本生体医工学会東北支部大会講演論文集,ME-B1-1
    • Place of Presentation
      東北大学 (仙台市)
    • Year and Date
      2012-11-17
  • [Presentation] 内分泌系の尺度に用いる唾液コルチゾール測定器の開発2012

    • Author(s)
      丹羽 大介,今井 義勝,浜地 健次,佐々木 誠,山口 昌樹
    • Organizer
      生活生命支援医療福祉工学系学会連合大会2012講演論文集 ,OS2-7-2
    • Place of Presentation
      名古屋大学 (名古屋市)
    • Year and Date
      2012-11-03
  • [Presentation] Fabrication of Semi-invasive Micro-needle Array Using Gradation Exposure2012

    • Author(s)
      M. Yamaguchi, Y. Sasaki, Y. Kimura, M. Sasaki
    • Organizer
      Pacific Rim Meeting on Electrochemical and Solid-state Science (PRiME2012)
    • Place of Presentation
      Hawaii Convention Center, Honolulu, HI (USA)(ECS Transactions: Chemical Sensors 8, No. 2029, The Electrochemical Society)
    • Year and Date
      2012-10-09
  • [Presentation] Detected Current Characteristics of Cortisol Sensor with Fluid Controlling Mechanism2012

    • Author(s)
      Shohei Sasaki, Yohei Matuda, Imai Yoshikatu, Daisuke Niwa, Masaki Yamaguchi
    • Organizer
      日本生体医工学会大会プログラム・論文集 ,Vol.50,Suppl.1,O2-05-2
    • Place of Presentation
      福岡国際会議場 (福岡市)
    • Year and Date
      2012-05-10
  • [Presentation] 唾液マーカーを用いたストレス可視化技術2012

    • Author(s)
      山口 昌樹
    • Organizer
      日本繊維製品消費科学会 第43回快適性を考えるシンポジウム
    • Place of Presentation
      京都市産業技術研究所 (京都市)
    • Year and Date
      2012-04-27
  • [Presentation] 非侵襲バイオマーカーを用いた空調機器の快適性評価2011

    • Author(s)
      山口 昌樹,吉野 雄一朗,佐々木 誠,西宮 肇
    • Organizer
      生活生命支援医療福祉工学系学会連合大会2011講演論文集 ,O1-1 (2011)
    • Place of Presentation
      芝浦工業大学 (港区)
    • Year and Date
      2011-11-03
  • [Presentation] 物質輸送と抗原抗体反応の連成解析によるイムノセンサの基礎設計2011

    • Author(s)
      木村 勇太,佐々木 誠,山口 昌樹
    • Organizer
      第45回日本生体医工学会東北支部大会講演論文集,ME-o3-1 (2011)
    • Place of Presentation
      岩手大学 (盛岡市)
    • Year and Date
      2011-10-29
  • [Presentation] 流体制御機構を備えたコルチゾールセンサの検出電流の評価2011

    • Author(s)
      佐々木 翔平,松田 陽平,今井 義勝,丹羽 大介,佐々木 誠,山口 昌樹
    • Organizer
      第45回日本生体医工学会東北支部大会講演論文集,ME-o1-3
    • Place of Presentation
      岩手大学 (盛岡市)
    • Year and Date
      2011-10-29
  • [Presentation] A Novel Analytical Technique of Skin Photo-stress Biomarker Using Surface Plasmon Resonance2010

    • Author(s)
      Masaki Yamaguchi, Tomoki Shimakura, Akira Date, Makoto Sasaki
    • Organizer
      5th Cairo International Biomedical Engineering Conference (CIBEC2010), 3050 (December, 2010) 240-243
    • Place of Presentation
      Sheraton Cairo Hotel, Cairo (Egypt)
    • Year and Date
      2010-12-18
  • [Presentation] 唾液による非侵襲的な生体情報計測2010

    • Author(s)
      山口 昌樹
    • Organizer
      次世代センサ協議会 次世代センサ総合シンポジウム
    • Place of Presentation
      東京ビッグサイト (江東区)
    • Year and Date
      2010-11-18
  • [Presentation] 唾液ストレス検査の展望2010

    • Author(s)
      山口 昌樹
    • Organizer
      第38回日本バイオフィードバック学会学術総会教育講演
    • Place of Presentation
      岩手大学 (盛岡市)
    • Year and Date
      2010-07-18
  • [Presentation] Digital Simulation of Immunosensor for Hormone Measurement Using Finite Element Method2010

    • Author(s)
      M. Yamaguchi, T. Takahashi, Y. Tahara, M. Sasaki, V. Shetty
    • Organizer
      The 13th International Meeting on Chemical Sensors (IMCS13)
    • Place of Presentation
      The University of Western Australia, Perth, Australia
    • Year and Date
      2010-07-12
  • [Presentation] 唾液ストレスマーカーの種類と使い方2010

    • Author(s)
      山口 昌樹
    • Organizer
      社団法人自動車技術会音質評価技術部門委員会
    • Place of Presentation
      中央大学 (文京区)
    • Year and Date
      2010-04-26
  • [Book] Human Olfactory Displays and Interfaces: Odor Sensing and Presentation, - Evaluating the Psychobiologic Effects of Fragrances through Salivary Biomarkers -2012

    • Author(s)
      Takamichi Nakamoto Eds., Masaki Yamaguchi and Vivek Shetty
    • Total Pages
      359-369 (555)
    • Publisher
      IGI Global, Hershey
  • [Book] 災害ストレスの対処法2011

    • Author(s)
      山口 昌樹,中島 康,中山 友紀
    • Total Pages
      69-114 (136)
    • Publisher
      講談社サイエンティフィク,東京
  • [Book] 体外診断用医薬品の開発と承認申請,第10章12節 唾液バイオマーカーによる非侵襲検査のニーズとシーズ2010

    • Author(s)
      菅原 隆編,山口 昌樹(分担執筆)
    • Total Pages
      325-334 (361)
    • Publisher
      (株)報協会,東京
  • [Book] Oral and Maxillofacial Surgery Clinics of North America, Collaborative Care of the Facial Injury Patient, - Salivary Biosensors for Screening Trauma related Psychopathology-2010

    • Author(s)
      Vivek Shetty and Grant N. Marshall Eds., Vivek Shetty and Masaki Yamaguchi
    • Total Pages
      269-278 (282)
    • Publisher
      Elsevier, Inc., New York
  • [Remarks]

    • URL

      http://www.mech.iwate-u.ac.jp/~yamaguchi/

  • [Patent(Industrial Property Rights)] 共振型質量センサ2013

    • Inventor(s)
      山口 昌樹
    • Industrial Property Rights Holder
      岩手大学
    • Industrial Property Number
      特願2013-28481
    • Filing Date
      2013-02-16
  • [Patent(Industrial Property Rights)] 検体採取器具2012

    • Inventor(s)
      今井 義勝,丹羽 大介,山口 昌樹
    • Industrial Property Rights Holder
      ローム(株),岩手大学
    • Industrial Property Number
      特願2012-085188
    • Filing Date
      2012-04-04
  • [Patent(Industrial Property Rights)] 金型,その製造方法,金型を用いた樹脂成形体の製造方法並びにその製造方法によって製造された樹脂成形体2010

    • Inventor(s)
      山口 昌樹,佐々木 誠,廣瀬 宏一
    • Industrial Property Rights Holder
      岩手大学
    • Industrial Property Number
      特許 、特願2010-211444
    • Filing Date
      2010-09-21

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi