2012 Fiscal Year Final Research Report
Development of the Novel Nano Trench-Patterned Culture Surface for Initiating Differentiation of Mesenchymal Stem Cell
| Project/Area Number |
22500430
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Waseda University |
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Principal Investigator |
TAKEDA Naoya 早稲田大学, 理工学術院, 准教授 (60338978)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 電子線リソグラフィー / 細胞パターニング / 間葉系幹細胞 / 分化誘導 / 人工ニッチ |
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| Research Abstract |
The novel cell patterning system was developed by utilizing the electron beam (EB) lithography and the EB-reactive polymer resists. And as a new method, we achieved inducing differentiation of mesechymal stem cell (MSC) just by culturing at the nano-topographic pattern of a single linear groove in 500 nm width, without transfecting any DNA and/or applying any biosignaling factor like concentional biological methods. MSCs were extraordinarily streched along the groove, presumably accompanying mechanical stress, and markedly increased gene expression of the neural markers and the osteogenic markers.
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[Remarks] (1)ICBS2013 Biomaterials Science Poster Prize (sponsored by RSC Publishing) : Aya Tsubokura, Yoshifumi Kawagishi, Satomi Aoki, Naoya Takeda, 2nd International Conference of Biomaterials Science in Tsukuba (ICBS2013), 2013. 学会受賞(学生発表)
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[Remarks] (2)優秀ポスター発表賞 : 坪倉彩, 川岸祥史, 武田直也, 第2 回CSJ 化学フェスタ, 2012 学会受賞(学生発表)
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[Remarks] (3)優秀ポスター発表賞 : 川岸祥史,吉野修弘, 武田直也, 第1 回CSJ 化学フェスタ―2011 世界化学年記念大会―, 2011 学会受賞(学生発表)
