2012 Fiscal Year Final Research Report
Stem cell culture system with immobilized signal proteins.
| Project/Area Number |
22500431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KITAJIMA Takashi 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 協力研究員 (40399556)
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| Research Collaborator |
JODDAR Binata 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 海外特別研究員
SIVAKUMAR Malliapan Ponnurengam 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 海外特別研究員
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| Project Period (FY) |
2010 – 2012
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| Keywords | 幹細胞 / 培養基材 / 増殖因子 / 増殖因子 / 増殖因子 |
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| Research Abstract |
Various signal proteins such as cytokines and growth factors were photo-immobilized on culture vessels to investigate the effect of them on stem cells. Immobilized LIF and MCP1 chemokine grow ES cells in undifferentiated state. Activin-A immobilized at high dose enhanced ES cells to differentiate into endodermal cells, and at lower dose to cells with mesodermal phenotype. Immobilized VEGF grow CD31 positive cells directly from mouse iPS cells without prior sorting of differentiating cells. These results suggest the possibility of culture devices for stem cells that can regulate differentiation.
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[Journal Article] Enhanced in vivo osteogenesis by nanocarrier- fused BMP42013
Author(s)
Shiozaki,Y., Kitajima,T., Mazaki, T., Yoshida Y., Tanaka M., Umezawa A., Nakamura M., Yoshida Y., Ito Y., Ozaki T., Matsukawa A.
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Journal Title
Int J Nanomed
Volume: 8
Pages: 1349-1360
Peer Reviewed
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[Journal Article] Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment2012
Author(s)
Kang, J., Sakuragi, M., Shibata, A., Abe, H., Kitajima, T., Tada, S., Mizutani, M., Ohmori, H., Ayame, H., Son, T.I., et al
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Journal Title
Materials Science and Engineering
Volume: C 32
Pages: 2552-2561
Peer Reviewed
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[Journal Article] Spatial immobilization of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold for enhanced osteoinductivity2012
Author(s)
Lu, H., Kawazoe, N., Kitajima, T., Myoken, Y., Tomita, M., Umezawa, A., Chen, G., and Ito, Y
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Journal Title
Biomaterials
Volume: 33
Pages: 6140-6146
Peer Reviewed
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[Journal Article] Addition of the collagen binding domain of fibronectin potentiates the biochemical availability of hepatocyte growth factor for cutaneous wound healing2011
Author(s)
Okiyama, N., Kitajima, T., Ito, Y., Yokozeki, H., Miyasaka, N., and Kohsaka, H
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Journal Title
J Dermatological Science
Volume: 61
Pages: 215-217
Peer Reviewed
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