2012 Fiscal Year Final Research Report
Observation of atrophied skeletal muscle using scanning electron microscopy
| Project/Area Number |
22500476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kitasato University |
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Principal Investigator |
SAKAMOTO Miki 北里大学, 医療衛生学部, 講師 (40365177)
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| Project Period (FY) |
2010 – 2012
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| Keywords | リハビリテーション医学 / 廃用性筋萎縮 / 走査型電子顕微鏡 |
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| Research Abstract |
This study analyzed the histological and ultrastructural changes of atrophied muscle fibers using scanning electron microscopy (SEM) and immunohistochemistry. We used an animal model of disused muscle atrophy. In muscle fibers of the immobilization group, wave-like structure was observed: it appeared as a groove, and was thought to be endomysium inserted into the cytoplasm of the muscle fiber. The wave-like structure was positive for anti-collagen IV antibody. Based on these results, the structure which inserted into the cytoplasm and formed a groove was confirmed to be endomysium. Moreover, myofibrillar disruption and MMP expression were not observed at region of the wave-like structure.
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