2012 Fiscal Year Final Research Report
The determination of optimal effective amount of the stretching in the cultured myoblast and elucidation of the mechanism of effects
| Project/Area Number |
22500491
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kibi International University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRAGAMI Fukumi 吉備国際大学, 保健医療福祉学部, 教授 (60278976)
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| Project Period (FY) |
2010 – 2012
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| Keywords | リハビリテーション / ストレッチ |
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| Research Abstract |
We performed 15-minute relatively short extension stimulation in cultured myoblast (H9c2 cell strain, C2C12 cell strain) mechanically and checked the influence of proliferation and differentiation. In addition, we assayed the expression of the RNAs of MyoD family (MyoD, Myogenin, Myf5, MRF4) with the RT-PCR and Myogenin protein and Myosin protein expression with the Western Blotting method. As a result, stretch stimulation did not adversely affect the proliferation in the skeletal muscle reproduction process, and exerting a positive effect on differentiation was revealed. It was suggested that MyoD RNA , Myogenin RNA and Myf5 RNA and Myogenin protein and Myosin protein played an important role for this.
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