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2012 Fiscal Year Final Research Report

Biological relevance of Coenzyme Q10 binding protein.-Study with prosaposin knockout animal and cell lines-

Research Project

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Project/Area Number 22500681
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied health science
Research InstitutionTokyo University of Technology

Principal Investigator

YAMAMOTO Yorihiro  東京工科大学, 応用生物学部, 教授 (60134475)

Co-Investigator(Kenkyū-buntansha) KASHIBA Misato  東京工科大学, 医療保健学部, 講師 (80338186)
Co-Investigator(Renkei-kenkyūsha) YOSHIMURA Shinichi  東海大学, 医学部, 講師 (30230808)
Project Period (FY) 2010 – 2012
Keywordsコエンザイム Q10 / プロサポシン / サポシン / 脂質 / 脂質結合タンパク質 / ノッ クアウトマウス
Research Abstract

Coenzyme Q10 (CoQ10) is necessary for ATP biosynthesis. It also is an antioxidant. Saposin B (SapB) is a CoQ10 binding protein.Analysis using prosaposin (Psap, precursor of SapB) knockout mouse revealed that concentrations of exogenous CoQ10 in plasma and liver were significantly reduced in homo-type animal. Psap transfectant (Tf) and knockdown (KD) HepG2 cells are established. CoQ10 contents decreased as follows: Tf > parent > KD. These data imply that SapB and/orits precursor Psap is a modulator for CoQ10 contents.

  • Research Products

    (16 results)

All 2013 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (9 results) Book (2 results)

  • [Journal Article] Carbon tetrachloride induced hepatic injury through the formation of oxidized diacylglycerol and its activation of the PKC/NF-κB pathway2013

    • Author(s)
      Toriumi K, Horikoshi Y, Henzan H, R. Osamura Y, Yamamoto Y, Nakamura N, Takekoshi S
    • Journal Title

      Lab Invest

      Volume: 93 Pages: 218-229

    • DOI

      doi:10.1038/labinvest.2012.145

    • Peer Reviewed
  • [Journal Article] Increased Oxidative Stress and Coenzyme Q10 Deficiency in Juvenile Fibromyalgia: Amelioration of Hypercholesterolemia and Fatigue by Ubiquinol-102013

    • Author(s)
      Miyamae T, Seki M, Naga T, Uchino S, Asazuma H, Yoshida T, Iizuka Y, Kikuchi M, Imagawa T, Natsumeda Y, Yokota S, Yamamoto Y
    • Journal Title

      Supplementation Redox Report

      Volume: 18 Pages: 12-19

    • DOI

      doi:10.1179/1351000212Y.0000000036

    • Peer Reviewed
  • [Journal Article] Hemodilution as a result of aggressive fluid resuscitation aggravates coagulopathy, but not survival, in a rat model of uncontrolled hemorrhagic shock2013

    • Author(s)
      Nishi K, Takasu A, Shinozaki H, Yamamoto Y, Sakamoto T
    • Journal Title

      J Trauma Acute Care Surg, g

      Volume: 74 Pages: 808-12

    • DOI

      doi:10.1097/TA.0b013e31827e1899

    • Peer Reviewed
  • [Journal Article] Adcock IM Goto H Up-regulation of blood arachidonate (20:4) levels in patients with chronic obstructive pulmonary disease2012

    • Author(s)
      Wada H, Hagiwara S, Saitoh E, Ieki E, Yamamoto Y
    • Journal Title

      Biomarkers

      Volume: 17 Pages: 520-523

    • DOI

      doi:10.3109/1354750X.2012.692393

    • Peer Reviewed
  • [Journal Article] Hypothermia reduces resuscitation fluid volumes required to maintain blood pressure in a rat hemorrhagic shock model2012

    • Author(s)
      Nishi K, Takasu A, Shibata M, Uchino S, Yamamoto Y, Sakamoto T
    • Journal Title

      J Trauma Acute Care Surg

      Volume: 72 Pages: 130-135

    • DOI

      doi:10.1097/TA.0b013e31821e61d8

    • Peer Reviewed
  • [Presentation] コエンザイムQ10結合タンパク質プロサポシンはビタミンEも結合する2012

    • Author(s)
      柿澤祐樹,山崎好裕,勇内山広樹,長谷川誠,森内寛,山本順寛
    • Organizer
      日本コエンザイムQ協会研究会発表場所
    • Place of Presentation
      東京都
    • Year and Date
      2012-01-24
  • [Presentation] コエンザイムQ結合蛋白質プロサポシン発現量改変株の酸化ストレスに対する感受性2012

    • Author(s)
      ジョン・チュンユン,長嶋康平,大泉美希子,鈴木優,森内寛,加柴美里,吉村眞一,山本順寛
    • Organizer
      日本コエンザイムQ協会研究会
    • Place of Presentation
      東京都
    • Year and Date
      2012-01-24
  • [Presentation] ヒト・プロサポシン過剰発現マウスにおけるコエンザイムQとビタミンEの体内動態2012

    • Author(s)
      関学,浅利真司,長尾美好,山本順寛,吉村眞一
    • Organizer
      日本コエンザイムQ協会研究会
    • Place of Presentation
      東京都
    • Year and Date
      2012-01-24
  • [Presentation] 母乳中のコエンザイムQ10とその結合タンパク質プロサポシン2012

    • Author(s)
      宮内優樹,関学,宮前多佳子,藤田秀次郎,石田史彦,森内寛,加柴美里,横田俊平,山本順寛
    • Organizer
      日本コエンザイムQ協会研究会
    • Place of Presentation
      東京都
    • Year and Date
      2012-01-24
  • [Presentation] プロサポシン遺伝子の改変で細胞内CoQ10の量とその酸化還元状態が変わる2011

    • Author(s)
      加柴美里,大泉美希子,鈴木優,澤村佳美,寺嶋政之,長嶋康平,及川慎吾,ジョン・チュンユン,森内寛,吉村眞一,山本順寛
    • Organizer
      日本酸化ストレス学会関東支部会
    • Place of Presentation
      東京都
    • Year and Date
      2011-12-10
  • [Presentation] Yamamoto YReduction of oxidative stress in successfully treated patients with community acquired pneumonia (CAP), as measured by redox status of coenzyme Q10 (%CoQ-10)2011

    • Author(s)
      Honda K, Wada H, Tamura M, Takata S, Nakamura M, Yasutake T, Kurai D, Ishii H, Goto H, Fujii K
    • Organizer
      European Respiratory SocietyMeeting
    • Place of Presentation
      Amsterdam, Nederland
    • Year and Date
      2011-09-30
  • [Presentation] ヒト・プロサポシン過剰発現マウスにおけるCoQ含量とサポシンBの組織内分布2011

    • Author(s)
      吉村眞一,長尾美好,浅利真司,川合巧真,関学,内野晋也,加柴美里,山本順寛,大塚正人,猪子英俊
    • Organizer
      日本コエンザイムQ協会研究会
    • Place of Presentation
      東京都
    • Year and Date
      2011-01-28
  • [Presentation] コエンザイムQ10結合タンパク質プロサポシンファミリーの生理的意義に関する考察2011

    • Author(s)
      山本順寛,加柴美里,吉村眞一
    • Organizer
      日本コエンザイムQ協会研究会
    • Place of Presentation
      東京都
    • Year and Date
      2011-01-28
  • [Presentation] Saposin B and its precursor protein prosaposin play key roles in absorption and transfer of CoQ102010

    • Author(s)
      Yorihiro Yamamoto, Misato Kashiba, and Shinichi Yoshimura
    • Organizer
      The 6th Conference of the InternationalCoenzyme 10 Association
    • Place of Presentation
      Bedford Hotel, Brussels, Belgium
    • Year and Date
      20100527-30
  • [Book] 機能性化粧品と薬剤デリバリー2013

    • Author(s)
      山本順寛
    • Publisher
      シーエムシー(印刷中)
  • [Book] Free Radical Biology in Digestive Diseases. Front Gastrointest Res2011

    • Author(s)
      Yorihiro Yamamoto
    • Total Pages
      64-70
    • Publisher
      Karger

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Published: 2014-08-29  

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