A general survey for teaching-aid kit design of biological teaching-materials and their apparatus by sterile condition

2012 Fiscal Year Final Research Report

• Project/Area Number: 22500805
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science education
• Research Institution: University of Fukui
• Principal Investigator: MAEDA Masuo 福井大学, 生命科学複合研究教育センター, シニアフェロー (10020140)
• Project Period (FY): 2010 – 2012
• Keywords: 科学教育 / 植物生殖現象 / 教材キット / シダ・裸子植物 / ミズワラビ・カニクサ / イチョウ / 顕微鏡 / 無菌技術

Research Abstract

A general survey for design of biological teaching-materials and their apparatus were especially experimented on reproduction process in plant. Subsequently, an in vitro kit was assembled and designed for biological teaching. Two species of fern (Ceratopteris richardii,and Lygodoium japonicum Sw.)were used for in vitro culture. The spores sterilized with routine techniques and subsequently desiccated spores were settled with pre-sterilized 1/2 Murashige and Skoog medium’s salts into the polystyrene vessel installed with ED-sterilized syringe filter on top portion of the vessel. The developing gametophyte (archegonium, antheridium, and sperm) can easily observe attaching the bottom of culture vessel with a magnifier or binocular. Sperm of Ginkgo bilobawere skillfully observed with hanging-drop culture method of pollen tube (spermatocyte) derived from young female seeds.