2013 Fiscal Year Final Research Report
Induction of Differentiation for Bone and Soft Tissue Sarcoma
| Project/Area Number |
22501043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical oncology
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| Research Institution | University of Toyama |
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Principal Investigator |
KANAMORI MASAHIKO 富山大学, 大学院医学薬学研究部(医学), 教授 (20204547)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Taketoshi 富山大学, 附属病院, 講師 (20377302)
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| Co-Investigator(Renkei-kenkyūsha) |
HORI Takeshi 飯山赤十字病院, 医師
SUZUKI Kayo 富山大学附属病院, 医員 (20456388)
MIYAHARA Tatsuro 富山大学, 医学部人間科学1講座, 協力研究員
KAWAGUCHI Makoto 上越労災病院, 病理学部門, 医師
NOGAMI Shigeharu 高岡市民病院, 整形外科, 医師
SANO Akimi 富山大学, 医学部整形外科, 技官
A. Julia Bridge University of Nebraska Medical Center, Nebraska, USA・Professor
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | がん / 分化誘導 / アポトーシス誘導 / 骨肉腫 / 骨軟部腫瘍 |
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| Research Abstract |
Genome-wide screening of array-based comparative genomic hybridization (array CGH) was used to investigate bone and soft tissue tumors. Our results may provide several entry points for the identification of candidate genes associated with aggressive change. Especially, the locus 17p11-13 including HIC1 close to p53 was common high amplification in this series. When human MOS cells are treated with 3mM dbcAMP for 24 hours, apoptotic changes were showed. From the finding of array CGH, amplification of SHGC17327 and LLGL1 and loss of OCRL1 are detected in the treated MOS cells.
We found the apoptotic changes of osteosarcoma cells in response to flavonol and flavanol in the screening examination of 11 flavonoids. In particular, ECG and EGCG induced sub-G1 increase, positivity of Anexin V, activation of caspase-3. ECG and EGCG with gallate-substrate provided the anti-proliferative effects that were stronger than any other catechins.
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