2012 Fiscal Year Final Research Report
Pancreas-specific antibody production and development of pancreatic cancer therapy using virus vector
| Project/Area Number |
22501054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical oncology
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Syouta 福岡大学, 医学部, 准教授 (90549338)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 癌 / 遺伝子治療 / 抗体療法 / 分子標的 |
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| Research Abstract |
We have recently developed the fiber modified adenovirus vector (Adv-FZ33). This vector can binds immunoglobulin-G (IgG) with high affinity. We demonstrated that monoclonal antibody (mAb) screened by Adv-FZ33, which is capable of binding to suitable target antigens on pancreatic cancer cell lines. Through this screening, we established twenty different mAbs. Among these mAbs, F2-27 is highly selective for cancer cell. The target antigens were identified as ** by MALDI-TOF mass spectrometry. These antigens were expressed in several cancer cell lines, including gastric, lung, and prostate cancer. We generated Ax3CMTK-FZ33 (tk-FZ33), which is an Adv-FZ33 derivative vector expressing a therapeutic gene, HSV-tk. tk-FZ33 with F2-27 enhanced the cytotoxicity of GCV (ganciclovir) compared with control mAb. These data suggest that F2-27-targeted FZ33-mutant adenovirus-mediated gene delivery offers a strong and selective therapeutic modality against cancers.
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[Journal Article] IKKβ regulates essential functions of the vascular endothelium through kinase-dependent and -independent pathways2011
Author(s)
Ashida N, Senbanerjee S, Kodama S, Foo SY, Coggins M, Spencer JA, Zamiri P, Shen D, Li L, Sciuto T, Dvorak A, Gerszten RE, Lin CP, Karin M, Rosenzweig A.
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Journal Title
Nat Commun
Volume: 2
Pages: 318
DOI
Peer Reviewed
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[Presentation] Inhibitory Effect of Thrombomodulin on HMGB1-Stimulated IFN-γ Production of Hepatic NKT and Gr-1+ Cells2012
Author(s)
Kojima D., Nishinakamura H., Ogata T., Nagaishi M., Mera T., Itoh T., Matsuoka N., Kodama S., Yasunami Y.
Organizer
Facilitating to Prevent Early Loss of Transplanted Islets in the Liver of Mice, 24th International Congress of the Transplantation society
Place of Presentation
Berlin, Germany
Year and Date
20120715-19
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