2012 Fiscal Year Final Research Report

Evaluation of nitrous oxide emission by aerobic methanotrophs as possible microbial members contributing to global warming.

Research Project

Project/Area Number	22510022
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Environmental dynamic analysis
Research Institution	Japan Agency for Marine-Earth Science and Technology
Principal Investigator	HIRAYAMA Hisako 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (90359167)
Co-Investigator(Kenkyū-buntansha)	TAKAI Ken 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクター (80359166)
Project Period (FY)	2010 – 2012
Keywords	メタン酸化菌 / 地球温暖化 / 亜酸化窒素
Research Abstract	The ability of nitrous oxide (N_2O) production by aerobic methanotrophic bacteria was examined by using pure cultures obtained mainly from marine environments. Methylobacter sp. strain, which was isolated in our laboratory from the deep-sea sediment, was revealed to produce significantly more N_2O than other strains tested. The addition of nitrite to the growth medium promoted production of N_2O. The presence of genes encoding hydroxylamine oxidoreductase was examined by DNA extracted from the strains, and the partial nucleotide sequences of the genes were determined.