2012 Fiscal Year Final Research Report
Analysis of regulatory mechanisms of DNA repair by ubiquitination
| Project/Area Number |
22510059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
MOTEGI Akira 京都大学, 大学院・医学研究科, 助教 (80452332)
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| Project Period (FY) |
2010 – 2012
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| Keywords | DNA修復 / 複製後修復 / 相同組換え / ユビキチン |
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| Research Abstract |
I have studied functions of the ubiquitin ligases SHPRH and RNF20 by genetically manipulatingthose genes in the chicken DT40 cell line. SHPRH/RAD18 double knockout cells showed enhancedsensitivity towards the DNA-crosslinking agent cisplatin, suggesting a cooperative role of SHPRH andRAD18 in repairing DNA crosslinks. Repression of the RNF20 transgene in RNF20-disrupted cellsresulted in reduced foci formation of the recombination factor RAD51 after ionizing radiation. Thus,RNF20 may facilitate repair of DNA double strand breaks by homologous recombination.
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[Journal Article] Simultaneous disruption of two DNApolymerases, Polη and Polζ, in avianDT40 cells unmasks the role of Polη incellular response to various DNAlesions2010
Author(s)
Hirota K, Sonoda E, Kawamoto T, MotegiA, Masutani C, Hanaoka F, Szuts D, IwaiS, Sale JE, Lehmann A, Takeda S
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Journal Title
PLoS Genet
Volume: 6
Pages: e1001151
Peer Reviewed
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