2012 Fiscal Year Final Research Report
Effects of DNA lesions on the transcription reaction of mitochondrial RNA polymerase
| Project/Area Number |
22510060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
KURAOKA Isao 大阪大学, 大学院・基礎工学研究科, 准教授 (60335396)
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| Co-Investigator(Renkei-kenkyūsha) |
IWAI Shigenori 大阪大学大学院, 基礎工学研究科, 教授 (10168544)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ミトコンドリア / RNAポリメラーゼ / 酸化DNA損傷 |
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| Research Abstract |
Mitochondrial RNA polymerase (mtRNAP) catalyzes the synthesis of essential transcripts in mitochondria where reactive oxidative (ROS) species are generated as byproducts. The occurrence of RNA synthesis by mtRNAP at oxidative DNA lesions remains unknown. Purified mtRNAP and a complex of RNA primer/DNA template containing a single DNA lesion were used for in vitro RNA synthesis assays. We show that mtRNAP bypassed the oxidative DNA lesion.
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[Journal Article] Miscoding properties of 8-ch loro-2′-deoxyguanosine, a hypochlorous aci d-induced DNA adduct, catalysed by huma n DNA polymerases.2013
Author(s)
Sassa A, Kamoshita N, Matsuda T, Ishi i Y, Kuraoka I, Nohmi T, Ohta T, Honma M, Yasui M
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Journal Title
Mutagenesis
Volume: 28
Pages: 81-88
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[Journal Article] Identification of small molecule prolife rating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box prot eins and inhibits DNA replication2012
Author(s)
Punchihewa C, Inoue A, Hishiki A, Fuji kawa Y, Connelly M, Evison B, Shao Y, Heath R, Kuraoka I, Rodrigues P, Hashimo to H, Kawanishi M, Sato M, Yagi T, Fujii N
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Journal Title
J Biol Chem
Volume: 287
Pages: 14289-14300
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[Journal Article] Characterization of a Y-family DNA polym erase eta from the eukaryotic thermophile Alvinella pompejana2010
Author(s)
Kashiwagi S, Kuraoka I, Fujiwara Y, Hit omi K, Cheng QJ, Fuss, JO, Shin, DS, Masutani C, Tainer, JA, Hanaoka F, Iwai S
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Journal Title
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[Journal Article] MMXD, a TFIIH-indepen dent XPD-MMS19 protein complex involved in chromosome segregation.2010
Author(s)
Ito S, Tan LJ, Andoh D, Narita T, Seki M, Hirano Y, Narita K, Kuraoka I, Hirao ka Y, Tanaka K
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Journal Title
Mol Cell
Volume: 39
Pages: 632-40
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