2012 Fiscal Year Final Research Report
Effect of light and dark dosing time on biological response to environmental chemicals in mice
| Project/Area Number |
22510070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | University of Shizuoka |
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Principal Investigator |
SHIMOI Kayoko 静岡県立大学, 環境科学研究所, 教授 (10162728)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKIBARA Hiroyuki 宮崎大学, 宮崎大学, 准教授 (20403701)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 生体リズム / 遺伝子発現 / 脂質代謝 / 遺伝毒性 / ベンツ[a]ピレン / ENU |
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| Research Abstract |
Any differences in the frequencies of micronucleus induction in peripheral blood reticulocytes(MNRETs) of mice exposed to BaP between light and dark dosing times were not observed. However, the expression of lipid metabolism related genes such as Fasn in liver was dramatically increased in treatment group at ZT15(darkphase). On the other hand, MNRETs of mice treated with ENU at ZT15 were significantly higher than those in mice treated at ZT3 (light phase). The expression of p53-responsible genes such as Cyclin G1 in the bone marrow cells was higher in treatment group at ZT3 than that at ZT15. DNA repair might be promoted at light phase. These results suggest that toxic sensitivity to chemicals is different between the light and dark phase.
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