• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Analysis of Wound Healing Process at the Single Cellular Level

Research Project

  • PDF
Project/Area Number 22510122
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionTokyo Institute of Technology

Principal Investigator

AFRIN Rehana  東京工業大学, 大学院・・理工学研究科, 東工 大特別研究員 (70447556)

Co-Investigator(Renkei-kenkyūsha) 猪飼 篤  東京工業大学, イノベーション研究推進体, 特任教授 (50011713)
Project Period (FY) 2010 – 2012
Keywords分子マニピュレーション / 細胞創傷治癒
Research Abstract

From the point of wound healing view, it is important to analyze the repair process of artificially inflicted damages to a live cell. We employed a small glass bead glued at the end of a thin tip of atomic force microscope and coated with enzyme with cell membrane hydrolyzing activity. When the glass bead touched the cell surface, it created a small hole on the cell membrane. First we studied the correlation between the time of repair of the hole and the hole diameter. We noticed that a certain time is required before repair process effectively started. We also noticed that bubble like structures were formed on the cell surface (blebs) and studied the growth and retraction process of them. We also studied the repair process of the damages given to intracellular stress fibers.

  • Research Products

    (24 results)

All 2013 2012 2011 2010

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Requirement of LIM domains for the transient accumulation of paxillin at damaged stress fibres2013

    • Author(s)
      Takahiro Watanabe- Nakayama, Masakazu Saito,Shin'ichi Machida, Kikuo Kishimoto, Rehana Afrin and Atsushi Ikai
    • Journal Title

      Biology Open

      Volume: (印刷中) Pages: (印刷中)

    • DOI

      doi:10.1242/bio.20134531

    • Peer Reviewed
  • [Journal Article] Interaction of Serum Proteins with Surface of Hemodialysis Fiber Membranes2012

    • Author(s)
      Rehana Afrin, Yuji Shirako, Kikuo Kishimoto and Atsushi Ikai
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: 51 Pages: 08KB10

    • DOI

      DOI10.1143/AP.51.08KB10

    • Peer Reviewed
  • [Journal Article] Force Extension of Delipidated Red Blood Cell Cytoskeleton with Little Indication of Spectrin Unfolding. Rehana Afrin2012

    • Author(s)
      Masato Nakaji, Hiroshi Sekiguchi,David Lee, Kikuo Kishimoto and Atsushi Ikai
    • Journal Title

      Cytoskeleton

      Volume: 69 Pages: 101-112

    • DOI

      DOI:10.1002/cm.21001

    • Peer Reviewed
  • [Journal Article] Fabricated Cantilever for AFM Measurements and manipulations:Pre-stress Analysis of Stress Fibers2012

    • Author(s)
      Shinichi Machida, Takahiro Watanabe- Nakayama, Masakazu Saito, Rehana Afrin and Atsushi Ikai
    • Journal Title

      Micron

      Volume: 43 Pages: 1380-1389.

    • DOI

      doi.org/10.1016/j.micron.2012.03.025

    • Peer Reviewed
  • [Journal Article] Mechanics of Intracellular Stress Fibers: A Short Review.2011

    • Author(s)
      Atsushi Ikai ,Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu Saito, and RehanaAfrin.
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: 50 Pages: 08LA04.

    • DOI

      DOI:10.1143/JJAP.50.08LA04

    • Peer Reviewed
  • [Journal Article] Direct Detection of Tension Recovery After Local Stretching of in Single Cell Level Surface.2011

    • Author(s)
      Takahiro W. Nakayama, Shin-ichi Machida, Ichiro Harada, Hiroshi Sekiguchi, Rehana Afrin, and Atsushi Ikai
    • Journal Title

      Biophys. J.

      Volume: 100 Pages: 564-572.

    • DOI

      doi:10.1016/j.bpj.2010.12.3693

    • Peer Reviewed
  • [Journal Article] Nonlinear Displacement of VentralStress Fibers Under an Externally AppliedLateral Force by an Atomic ForceMicroscope.2011

    • Author(s)
      Tomoro Hakari, HiroshSekiguchi, Toshiya Osada, KikuKishimoto, Rehana Afrin, and Atsus Ikai.
    • Journal Title

      Cytoskeleton

      Volume: 68 Pages: 628-638

    • DOI

      DOI:10.1002/cm.20537

    • Peer Reviewed
  • [Journal Article] Micro-scoop for Manipulation of Micro Objects: Use of Fabricated Cantilever with Atomic Force Microscope2010

    • Author(s)
      Takahiro Watanabe-Nakayama, Shin-ichi Machida, Rehana Afrin, and Atsushi Ikai
    • Journal Title

      Small

      Volume: 6 Pages: 2853-2857.

    • DOI

      DOI:10.1002/smll.201001632

    • Peer Reviewed
  • [Journal Article] Direct Manipulation of Intracellular Stress Fibers Using a Hook-shaped AFM probe.2010

    • Author(s)
      Shinichi Machida, Takahiro Watanabe-Nakayama, IchiroHarada, Rehana Afrin , Tomonobu Nakayamaand Atsushi Ikai
    • Journal Title

      Nanotechnology

      Volume: 21 Pages: 385102.

    • DOI

      doi:10.1088/0957-4484/21/38/385102

    • Peer Reviewed
  • [Journal Article] Molecular Shape and Binding Force of Mycoplasma Mobile's Leg Protein Gli349 Revealed by an AFM Study.2010

    • Author(s)
      Charles Lesoil,Takahiro Nonaka, Hiroshi Sekiguchi, Toshiya Osada, Makoto Miyata,RehanaAfrin and Atsushi Ikai.
    • Journal Title

      Biochem.Biophys. Res. Commun.

      Volume: 391 Pages: 1312-1317.

    • DOI

      doi:10.1016/j.bbrc.2009.12.023.

    • Peer Reviewed
  • [Presentation] Rehana Afrin , Exploring Atomic Force Microscopy for Single Cell Manipulations.2012

    • Organizer
      Microscopy & Microanalysis (M&M) 2012 Meeting
    • Place of Presentation
      Phoenix, AZ, USA.
    • Year and Date
      20120729-0802
  • [Presentation] Rehana Afrin,Exploring Atomic Force Microscopyfor Nano-medical Applications.2012

    • Organizer
      ACMM22/ICONN2012/APMC10 Combined Conference Perth
    • Place of Presentation
      WA, Australia.
    • Year and Date
      20120205-09
  • [Presentation] Rehana Afrin, Interaction of Serum Proteins with the Surface of Hemodialysis Tubing.2011

    • Organizer
      International Colloquium of Scanning Probe Microscopy (ICSPM19)
    • Place of Presentation
      Toyako, Hokkaido, Japan.
    • Year and Date
      20111219-21
  • [Presentation] Rehana Afrin, Exploring Atomic Force Microscopy for Single Cell Manipulations.2011

    • Organizer
      Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      Denver, CO, USA.
    • Year and Date
      20111203-07
  • [Presentation] Rehana Afrin, Interaction Between Serum Proteins And Polymer Surface Studied By Atomic Force Microscopy.2011

    • Organizer
      AFM-BioMed Conference
    • Place of Presentation
      Paris, France.
    • Year and Date
      20110823-27
  • [Presentation] Rehana Afrin,Atomic Force Microscopy for Nano-medical Applications.2011

    • Organizer
      2nd International Nano Medicine Conference.
    • Place of Presentation
      Sydney, NSW, Australia.
    • Year and Date
      20110714-16
  • [Presentation] Rehana Afrin,Mechanical Interactions of Red Blood Cell Cytoskeleton and Associated Proteins.2011

    • Organizer
      13th International Scanning Probe Microscopy Conference(ISPM)
    • Place of Presentation
      Munich, Germany.
    • Year and Date
      20110619-22
  • [Presentation] Rehana Afrin, Surface Morphology Study of Human Skin Cells with AFM and SEM2011

    • Organizer
      55th Annual Meeting of the American Biophysical Society, Baltimore
    • Place of Presentation
      MD, USA.
    • Year and Date
      20110303-09
  • [Presentation] Rehana Afrin, Direct Manipulation Of Cytoskeletal Structures By Atomic Force Microscopy".2010

    • Organizer
      50th Annual Meeting of American Society of Cell Biology (ASCB)
    • Place of Presentation
      Philadelphia, PA. USA
    • Year and Date
      20101210-15
  • [Presentation] Rehana Afrin, Exploring Atomic Force Microscopy for Single Cell Manipulations as a Novel Nanodevice for Nanomedical Application.2010

    • Organizer
      1st World Congress of Nanomedicine.
    • Place of Presentation
      Beijing, CHINA.
    • Year and Date
      20101022-26
  • [Presentation] Rehana Afrin, Direct Manipulation of Live Cells and Cytoskeletal Structure with Atomic Force Microscope.2010

    • Organizer
      IMC 17, Rio De Janeiro
    • Place of Presentation
      BRAZIL.
    • Year and Date
      20100915-19
  • [Presentation] Rehana Afrin, Tensile Property of Delipidated Red Blood Cell Cytoskeleton".2010

    • Organizer
      12th International Scanning Probe Microscopy Conference (ISPM)
    • Place of Presentation
      Sapporo, JAPAN.
    • Year and Date
      20100510-12
  • [Book] Chapter 15.Nano-surgical Manipulation of Living Cells with the AFM" in "Atomic Force Microscopy in liquid"2012

    • Author(s)
      Atsushi Ikai, Rehana Afrin, Takahiro Nakayama and Shin-ichi Machida
    • Total Pages
      362.
  • [Book] Chapter 12 Mapping Membrane Proteins on Living Cells Using The Atomic Force Microscope in "Life at the Nanoscale: Atomic Force Microscopy of Live Cells"2011

    • Author(s)
      Atsushi Ikai_ and _Rehana Afrin
    • Total Pages
      444..

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi