2012 Fiscal Year Final Research Report
Analysis of a novel life-duration related gene which was discovered by high-through-put generation of deletion mutants
Project/Area Number |
22510202
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genome biology
|
Research Institution | University of Tsukuba |
Principal Investigator |
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Project Period (FY) |
2010 – 2012
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Keywords | 微生物ゲノム / 遺伝子の機能解析 |
Research Abstract |
In this study, we aimed to identify the regulatory pathway a novel life-duration related gene, rabgap. We clarified that the product interacts with Gα2 and Gα9 in a GTP-dependent manner by pull-down assay. Biochemical analysis revealed that the RabGAP gene product regulates cAMP synthesis which is a driving force for Dictyostelium discoideum development. Furtheremore, it was found that arachidonic acid synthesis pathway also contributes the cAMP synthesis. We also suggested that RabGAP participates in Lysosomal pathway and regulates cell proliferation probably via Gα9.
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